Involvement of P2Y₁₂ receptors in a nitroglycerin‐induced model of migraine in male mice

Abstract

Supporting information: 5 Figures, 2 Tables and methods Authors contributions: FG carried out behaviour, IHC, FACS experiments, analysed data, performed statistical analyses, and drafted the manuscript. MB conducted HPLC analysis, AI and LH performed blood pressure and heart rate measurement, and analysed the data. LO carried out confocal microscopy and revised the manuscript. ZB designed experiments and revised the manuscript. BS designed and supervised the study and finalized the paper. All authors read and approved the final manuscript.

Single-cell RNA sequencing reveals distinct transcriptional features of the purinergic signaling in mouse trigeminal ganglion

“…Previous studies showed that activation of P2Y12R in SGCs of TG was in involved in orofacial neuropathic pain induced by unilateral lingual nerve injury ( Katagiri et al, 2012 ; Sugawara et al, 2017 ). In addition, microglia P2Y12R in STN was also verified to be involved in several types of orofacial pain, including migraine and tongue cancer pain ( Tamagawa et al, 2016 ; Gölöncsér et al, 2021 ). In our studies, P2ry12 was expressed not only in SGCs, but also in macrophages and MLCs, suggesting that the pro-nociceptive effect of P2Y12R may not only rely on the glia-neuron interactions but also on the neuroimmune communication.…”

Ion Channel Disturbances in Migraine Headache: Exploring the Potential Role of the Kynurenine System in the Context of the Trigeminovascular System

“…NTG activates and sensitizes the trigeminal system [107,108], central mechanism crucial in migraine pathophysiology, as described earlier. In a recent study, it was shown that inhibition of P2Y12 receptors with the selective antagonist PSB-0739 decreases c-Fos expression in the NTG model of migraine pain in mice [109], which underlines the possible role of P2Y receptors in migraine pathomechanism.…”

Antiplatelet and Antithrombotic Effects of Isaridin E Isolated from the Marine-Derived Fungus via Downregulating the PI3K/Akt Signaling Pathway

“…Mice were assigned randomly to 7 groups and intragastrically administrated with a single dose of vehicle, isaridin E (12.5, 25, 50, or 100 mg/kg), or clopidogrel (10 mg/kg) at 3 h before the cutting-tail experiment [42,43]. Tails were transected at a distal margin of 5 mm and were immediately immersed into 10 mL of saline at 37 • C. The bleeding time was recorded as when the observed bleeding ceased.…”