Involvement of Noncoding RNAs in the Differentiation of Osteoclasts

Zhao, Yi; Jia, Lingfei; Zheng, Yunfei; Li, Weiran

doi:10.1155/2020/4813140

Cited by 7 publications

(4 citation statements)

References 204 publications

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“…miRNAs were stated to have relevant effects in controlling osteoclast differentiation. Research reported that an increase of miR-125a-5p augmented osteoclast differentiation through blocking TNFRSF1B expression [ 74 ], while Zhou et al demonstrated that the increase of miR-100-5p avoided bone loss in ovariectomized animals through reducing the production of FGF-21 and osteoclast activeness [ 75 ].…”

Section: Introductionmentioning

confidence: 99%

The Osteoporosis/Microbiota Linkage: The Role of miRNA

Martinis

Ginaldi

Allegra

et al. 2020

IJMS

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Hundreds of trillions of bacteria are present in the human body in a mutually beneficial symbiotic relationship with the host. A stable dynamic equilibrium exists in healthy individuals between the microbiota, host organism, and environment. Imbalances of the intestinal microbiota contribute to the determinism of various diseases. Recent research suggests that the microbiota is also involved in the regulation of the bone metabolism, and its alteration may induce osteoporosis. Due to modern molecular biotechnology, various mechanisms regulating the relationship between bone and microbiota are emerging. Understanding the role of microbiota imbalances in the development of osteoporosis is essential for the development of potential osteoporosis prevention and treatment strategies through microbiota targeting. A relevant complementary mechanism could be also constituted by the permanent relationships occurring between microbiota and microRNAs (miRNAs). miRNAs are a set of small non-coding RNAs able to regulate gene expression. In this review, we recapitulate the physiological and pathological meanings of the microbiota on osteoporosis onset by governing miRNA production. An improved comprehension of the relations between microbiota and miRNAs could furnish novel markers for the identification and monitoring of osteoporosis, and this appears to be an encouraging method for antagomir-guided tactics as therapeutic agents.

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Section: Introductionmentioning

confidence: 99%

The Osteoporosis/Microbiota Linkage: The Role of miRNA

Martinis

Ginaldi

Allegra

et al. 2020

IJMS

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“…Non-coding RNAs (ncRNAs) are a crucial type of RNA that are involved in various genetic and epigenetic processes such as transcriptional and post-transcriptional regulation ( Geng et al, 2018 ; Panni et al, 2020 ). Interestingly, despite the fact that only 10% of the genome codes for proteins, most of it is transcribed into functional non-coding RNAs ( Zhao et al, 2020 ). NcRNAs can be classified into basic structure and regulatory types, and they play a significant role in both physiological and pathological processes ( Li M. et al, 2018 ), including the regulation of neuropathic pain and activation of Wnt/β-catenin signaling in the spinal cord ( Sun et al, 2018 ; Cui et al, 2019 ; Zhang S. B. et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profile analysis in spinal cord injury rats with transplantation of menstrual blood-derived stem cells

Qi,

Jiang,

et al. 2024

Front. Mol. Neurosci.

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IntroductionMenstrual blood-derived stem cells (MenSCs) are vital in treating many degenerative and traumatic disorders. However, the underlying molecular mechanisms remain obscure in MenSCs-treating spinal cord injury (SCI) rats.MethodsMenSCs were adopted into the injured sites of rat spinal cords at day 7 post surgery and the tissues were harvested for total RNA sequencing analysis at day 21 after surgery to investigate the expression patterns of RNAs. The differentially expressed genes (DEGs) were analyzed with volcano and heatmap plot. DEGs were sequentially analyzed by weighted gene co-expression network, functional enrichment, and competitive endogenous RNAs (ceRNA) network analysis. Next, expression of selected miRNAs, lncRNAs, circRNAs and mRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatics packages and extra databases were enrolled to scoop the genes functions and their interaction relationships.ResultsA total of 89 lncRNAs, 65 circRNAs, 120 miRNAs and 422 mRNAs were significantly upregulated and 65 lncRNAs, 72 circRNAs, 74 miRNAs, and 190 mRNAs were significantly downregulated in the MenSCs treated rats compared to SCI ones. Current investigation revealed that MenSCs treatment improve the recovery of the injured rats and the most significantly involved pathways in SCI regeneration were cell adhesion molecules, nature killer cell mediated cytotoxicity, primary immunodeficiency, chemokine signaling pathway, T cell receptor signaling pathway and B cell receptor signaling pathway. Moreover, the lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA ceRNA network of SCI was constructed. Finally, the protein-protein interaction (PPI) network was constructed using the top 100 DE mRNAs. The constructed PPI network included 47 nodes and 70 edges.DiscussionIn summary, the above results revealed the expression profile and potential functions of differentially expressed (DE) RNAs in the injured spinal cords of rats in the MenSCs-treated and SCI groups, and this study may provide new clues to understand the mechanisms of MenSCs in treating SCI.

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“…By attaching to its receptor, RANK, RANKL plays a critical role in regulating mature osteoclast development, function, and survival. Moreover, osteoclastogenesis relies on the transcriptional activity of NFATc1, cathepsin K (CTSK), and c‐Fos, all of which are triggered by the nuclear factor‐κB (NF‐κB) signaling pathways, which are mobilized in part thanks to RANK recruitment (Yu et al, 2015 ; Zhao et al, 2020 ). Therefore, it is imperative to suppress RANKL‐induced downstream signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Orientin suppresses osteoclastogenesis and ameliorates ovariectomy‐induced osteoporosis via suppressing ROS production

Zheng

Wang

Pan

et al. 2023

Food Science & Nutrition

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The aberrant differentiation of osteoclasts is a key feature of the pathogenesis of osteoporosis, which has a devastating impact on human health. While the effects of Orientin (Ori) on osteoporosis, particularly on RANKL‐stimulated osteoclast production and activation, remain still unclear, Ori has been found to display several biological activities, including antioxidant and anti‐inflammatory. In this work, we investigated the possible pathways through which Ori suppressed RANKL‐induced osteoclast development and showed for the first time that it does so. The macrophages from the bone marrow (BMMs) were cultivated and then treated with Ori after being stimulated with RANKL. Then, TRAP‐positive multinucleated cells were counted, and F‐actin ring analysis was used to assess Ori's impact on mature osteoclast development. In addition, dihydroethidium (DHE) staining was used to evaluate the impact of Ori on RANKL‐induced reactive oxygen species (ROS). In addition, we performed western blotting and quantitative RT‐PCR analysis to investigate probable causes of these downregulation effects. We discovered that Ori inhibits the creation of osteoclasts, the gene and protein expressions unique to osteoclasts, and the ROS production. By activating Nrf2 and other ROS‐scavenging enzymes, Ori reduces intracellular ROS levels. The expression of the main transcription factor of osteoclast development, c‐Fos, was downregulated together with NFATc1, CTSK, and NFATc2, thanks to Ori's inhibition of RANKL‐induced NF‐κB. Consistent with its in vitro antiosteoclastogenic action, Ori therapy in the ovariectomized (OVX) rat model was also able to restore bone mass and improve microarchitecture in the distal femurs. Together, our results demonstrate that Ori is a flavonoid molecule with therapeutic promise for bone illnesses associated with osteoclasts, such as osteoporosis.

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Involvement of Noncoding RNAs in the Differentiation of Osteoclasts

Cited by 7 publications

References 204 publications

The Osteoporosis/Microbiota Linkage: The Role of miRNA

The Osteoporosis/Microbiota Linkage: The Role of miRNA

Transcriptome profile analysis in spinal cord injury rats with transplantation of menstrual blood-derived stem cells

Orientin suppresses osteoclastogenesis and ameliorates ovariectomy‐induced osteoporosis via suppressing ROS production

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