Involvement of NO in the inhibitory effect of Calotropis procera latex protein fractions on leukocyte rolling, adhesion and infiltration in rat peritonitis model

Ramos, Márcio V.; Oliveira, Jefferson Soares de; Figueiredo, J. G.; Figueiredo, Ingrid Samantha Tavares de; Kumar, Vijay; Bitencourt, Flavio Silveira; Cunha, Fernando; Oliveira, Raquel Sombra Basílio de; Bomfim, Liezelotte R.; Lima‐Filho, José Vitor; Alencar, Nylane Maria Nunes de

doi:10.1016/j.jep.2009.07.030

Cited by 28 publications

(27 citation statements)

References 40 publications

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“…The laticifer fluid of C. procera encompasses a large amount of proteins with anti-inflammatory properties and prospective pharmacological uses [11,18]. We have shown that the enhanced protection by LP or LP PI inoculums against Salmonella infection was not related to protease activity.…”

Section: Discussionmentioning

confidence: 91%

“…The fractions corresponding to these peaks were dialyzed against distilled water and lyophilized. In the present study, LP PI was used because previous data have shown that this fraction is highly antiinflammatory [18].…”

Section: Procera Proteinsmentioning

confidence: 99%

“…While these LP have been fractionated by ionexchange chromatography and biochemically characterized, the aim of the present study was to investigate the effect of one sub-fraction, named LP PI , on the protection rates and inflammatory response of Swiss mice infected by S. Typhimurium. This fraction was chosen because its administration was highly anti-inflammatory, decreasing the number of rolling and adherent leukocytes in the mesenteric microvasculature of animal models [18]. The influence of LP PI on NO release, cytokine mRNA expression and neutrophil recruitment are discussed in light of previous biological activities reported for C. procera proteins.…”

Section: Introductionmentioning

confidence: 99%

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Inflammation induced by phytomodulatory proteins from the latex of Calotropis procera (Asclepiadaceae) protects against Salmonella infection in a murine model of typhoid fever

et al. 2012

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Section: Discussionmentioning

confidence: 91%

Section: Procera Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inflammation induced by phytomodulatory proteins from the latex of Calotropis procera (Asclepiadaceae) protects against Salmonella infection in a murine model of typhoid fever

et al. 2012

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“…Inflammatory response associated with sepsis is well known to promote coagulation, and under these conditions, owing to the anti-inflammatory property of LP present in its subfraction LP PI , it produced a marked increase in the coagulation time as compared to septic control (Ramos et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Soluble proteins (latex proteins, LP) were recovered from the whole latex according to the method described by Lima-Filho et al (2010). LP was further fractionated by ion-exchange chromatography on a CM-Sepharose fast flow column to obtain three subfractions named LP PI , LP PII , and LP PIII according to Ramos et al (2009).…”

Section: Latex Protein Isolation and Ion-exchange Chromatographymentioning

confidence: 99%

Proteins derived from latex of C. procera maintain coagulation homeostasis in septic mice and exhibit thrombin- and plasmin-like activities

Ramos

Viana

Silva

et al. 2012

Naunyn-Schmiedeberg's Arch Pharmacol

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The proteins derived from the latex (LP) of Calotropis procera are well known for their anti-inflammatory property. In view of their protective effect reported in the sepsis model, they were evaluated for their efficacy in maintaining coagulation homeostasis in sepsis. Intraperitoneal injection of LP markedly reduced the procoagulation and thrombocytopenia observed in mice infected with Salmonella; while in normal mice, LP produced a procoagulant effect. In order to understand its mechanism of action, the LP was subjected to ion-exchange chromatography, and the three subfractions (LPPI, LPPII, and LPPIII) thus obtained were tested for their proteolytic effect and thrombin- and plasmin-like activities in vitro. Of the three subfractions tested, LPPII and LPPIII exhibited proteolytic effect on azocasein and exhibited procoagulant effect on human plasma in a concentration-dependent manner. Like trypsin and plasmin, these subfractions produced both fibrinogenolytic and fibrinolytic effects that were mediated through the hydrolysis of the Aα, Bβ, and γ chains of fibrinogen and α-polymer and γ-dimer of fibrin clot, respectively. This study shows that the cysteine proteases present in the latex of C. procera exhibit thrombin- and plasmin-like activities and suggests that these proteins have therapeutic potential in various conditions associated with coagulation abnormalities.

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Protective Effect of Proteins Derived from the Latex of Calotropis procera against Inflammatory Hyperalgesia in Monoarthritic Rats

Kumar

Chaudhary

Ramos

et al. 2011

Phytotherapy Research

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Calotropis procera (family: Apocynaceae) is a plant growing in the wild and has been used in the traditional medicinal system for the treatment of various diseases. The plant produces milky latex that possesses potent antiinflammatory and analgesic properties. In present study the non-dialysable protein fraction isolated from the latex (LP) of this plant was evaluated for its efficacy against inflammation in rats where paw edema was induced by sub-plantar injection of carrageenin or monoarthritis was induced by intra-articular injection of Freund's complete adjuvant (FCA). The effect of LP was evaluated on edema volume in the paw model and on joint diameter, stair climbing ability, motility, dorsal flexion pain, levels of oxidative stress markers and joint histology in arthritis model. The protection afforded by LP was compared with that of standard antiinflammatory drug, diclofenac (5 mg/kg). LP exhibited a dose-dependent antiinflammatory effect and produced 32% and 60% inhibition of paw edema at 10 and 25 mg/kg doses and 12% and 36% inhibition of joint inflammation at 50 and 150 mg/kg doses. The protective effect of LP was associated with normalization of joint functions, histology and levels of oxidative stress markers in joint tissue. The findings of this study suggest that the protein fraction of latex of Calotropis procera has the potential to relieve inflammation and pain associated with various arthritic conditions.

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Involvement of NO in the inhibitory effect of Calotropis procera latex protein fractions on leukocyte rolling, adhesion and infiltration in rat peritonitis model

Abstract: Our study shows that LP fraction of Calotropis procera comprises of three distinct sets of proteins exhibiting anti-inflammatory and anti-nociceptive properties of which LP(PI) was most potent in inhibiting neutrophil functions and its effects are mediated through NO production.

Cited by 28 publications

References 40 publications

Inflammation induced by phytomodulatory proteins from the latex of Calotropis procera (Asclepiadaceae) protects against Salmonella infection in a murine model of typhoid fever

Inflammation induced by phytomodulatory proteins from the latex of Calotropis procera (Asclepiadaceae) protects against Salmonella infection in a murine model of typhoid fever

Proteins derived from latex of C. procera maintain coagulation homeostasis in septic mice and exhibit thrombin- and plasmin-like activities

Protective Effect of Proteins Derived from the Latex of Calotropis procera against Inflammatory Hyperalgesia in Monoarthritic Rats

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