Involvement of nitric oxide pathways in short term modulation of tyrosine hydroxylase activity by endothelins 1 and 3 in the rat anterior hypothalamus

Morgazo, Carolina; Perfume, Guadalupe; Legaz, Guillermina; Nunzio, Andrea di; Hope, Sandra; Bianciotti, Liliana G.; Vatta, Marcelo S.

doi:10.1016/j.bbrc.2005.06.168

Cited by 11 publications

(13 citation statements)

References 39 publications

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“…Our results support that ET-1 and ET-3 regulate catecholaminergic transmission in the hypothalamus by controlling the synthesis (11,12), uptake (unpublished observations), and release of NE (33,41). These findings suggest that ETs modulate NE turnover.…”

Section: Ajp-regul Integr Comp Physiolsupporting

confidence: 86%

“…Various reports support that the NO pathway is coupled to ET B receptor activation (9,40). In this sense, we reported that in the AHR, both ETs decrease NE release and participate in the short-term regulation of TH activity through the activation of ET B receptors coupled to the NO pathway (11,33). Present findings show that in the AHR, ET B receptors also mediated the long-term effect of ETs on TH activity since the reduction was abolished by a selective antagonist (BQ-788) and mimicked by an agonist (IRL-1620).…”

Section: Discussionmentioning

confidence: 56%

“…1). We previously reported that at 30 min ETs diminish TH activity in both hypothalamic regions (33,41). However, the time course study on TH activity in the AHR showed that ET-1 and ET-3 (10 nM) diminished the activity of the enzyme at 60, 120, 240, and 360 min.…”

Section: Resultsmentioning

confidence: 85%

“…We previously reported that the NO pathway mediates ET-1 and ET-3 short-term regulation of TH in both hypothalamic regions (33,41). The participation of the NO/cGMP/PKG pathway in ET response was evaluated by pretreatment with 10 M L-NAME, 10 M 7-NI, 10 M ODQ, and 2 M KT 5823, (inhibitors of NOS, NOS neuronal isoform, NO sensitive guanylyl cyclase, and PKG, respectively).…”

Section: Resultsmentioning

confidence: 99%

“…It was shown that ET-1 increases dopamine release in the rat striatum, whereas ET-3 augments catecholamine output in cortical, as well as striatal brain slices (27,52). We previously reported that both ETs modulate NE release, as well as the short-term modulation of TH activity and expression of the phosphorylated forms of the enzyme in the AHR and PHR (11,12,33,41).…”

mentioning

confidence: 97%

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Long-term modulation of tyrosine hydroxylase activity and expression by endothelin-1 and -3 in the rat anterior and posterior hypothalamus

Perfume

Nabhen²,

Barrera³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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Perfume G, Nabhen SL, Riquelme Barrera K, Otero MG, Bianciotti LG, Vatta MS. Long-term modulation of tyrosine hydroxylase activity and expression by endothelin 1 and 3 in the rat anterior and posterior hypothalamus. Am J Physiol Regul Integr Comp Physiol 294: R905-R914, 2008. First published December 19, 2007 doi:10.1152/ajpregu.00555.2007.-Brain catecholamines are involved in the regulation of biological functions, including cardiovascular activity. The hypothalamus presents areas with high density of catecholaminergic neurons and the endothelin system. Two hypothalamic regions intimately related with the cardiovascular control are distinguished: the anterior (AHR) and posterior (PHR) hypothalamus, considered to be sympathoinhibitory and sympathoexcitatory regions, respectively. We previously reported that endothelins (ETs) are involved in the short-term tyrosine hydroxylase (TH) regulation in both the AHR and PHR. TH is crucial for catecholaminergic transmission and is tightly regulated by well-characterized mechanisms. In the present study, we sought to establish the effects and underlying mechanisms of ET-1 and ET-3 on TH long-term modulation. Results showed that in the AHR, ETs decreased TH activity through ETB receptor activation coupled to the nitric oxide, phosphoinositide, and CaMK-II pathways. They also reduced total TH level and TH phosphorylated forms (Ser 19 and 40). Conversely, in the PHR, ETs increased TH activity through a G protein-coupled receptor, likely an atypical ET receptor or the ETC receptor, which stimulated the phosphoinositide and adenylyl cyclase pathways, as well as CaMK-II. ETs also increased total TH level and the Ser 19, 31, and 40 phosphorylated sites of the enzyme. These findings support that ETs are involved in the long-term regulation of TH activity, leading to reduced sympathoinhibition in the AHR and increased sympathoexcitation in the PHR. Present and previous studies may partially explain the cardiovascular effects produced by ETs when applied to the brain. catecholamines; endothelin receptors; central nervous system ENDOTHELINS (ETS) ARE VASOACTIVE peptides originally isolated from cultured porcine endothelial cells (56). They constitute a family of three 21-amino acid isopeptides termed ET-1, ET-2, and ET-3 (10, 23). ETs exert their biological effects through the activation of ET A and ET B receptors that are G proteincoupled receptors (GPCRs) (10,34,38). Despite these two well pharmacologically characterized GPCRs, several pharmacological and functional studies support the existence of additional ET receptors. The finding of atypical responses in the presence of selective ET A and ET B antagonists and/or agonists observed in different studies built up the term atypical receptors (ET AX or ET BX ) to refer to those receptors that mediate ET responses but fail to be identified by using selective antagonists or agonists of the conventional ET A and ET B receptors (12,20,36,38,41). In addition, a third receptor subtype named ET C that displays high affinity for ET-3 was ...

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Section: Ajp-regul Integr Comp Physiolsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 97%

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Long-term modulation of tyrosine hydroxylase activity and expression by endothelin-1 and -3 in the rat anterior and posterior hypothalamus

Perfume

Nabhen²,

Barrera³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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Short-term Effects of Endothelins on Tyrosine Hydroxylase Activity and Expression in the Olfactory Bulb of Normotensive Rats

et al. 2008

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The olfactory system in rats is part of the limbic region with extensive afferent connections with brain areas involved in the regulation of behaviour and autonomic responses. The existence of the endothelin system and catecholaminergic neurons in the olfactory bulb suggests that endothelins may modulate noradrenergic transmission and diverse olfactory mediated processes. In the present work we studied the effect of endothelin-1 and -3 on neuronal norepinephrine release and the short-term regulation of tyrosine hydroxylase in the olfactory bulb. Results showed that both endothelins increased tyrosine hydroxylase activity through the activation of a non-conventional endothelin G-protein coupled receptor, coupled to the stimulation of protein kinase A and C, as well as Ca(2+)/calmodulin-dependent protein kinase II. On the other hand, neither endothelin-1 nor endothelin-3 modified tyrosine hydroxylase total protein levels, but both peptides increased the phosphorylation of serine residues of the enzyme at sites 19 and 40. Furthermore, endothelins enhanced norepinephrine release in olfactory neurons suggesting that this event may contribute to increased tyrosine hydroxylase activity by reducing the feedback inhibition. Taken together present findings show a clear interaction between the endothelin system, and the catecholaminergic transmission in the olfactory bulb. Additional studies are required to evaluate the physiological functions regulated by endothelins at this brain level.

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Regulation of the Norepinephrine Transporter by Endothelins

Vatta¹,

Bianciotti²,

Guil³

et al. 2015

Vitamins &Amp; Hormones

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Involvement of nitric oxide pathways in short term modulation of tyrosine hydroxylase activity by endothelins 1 and 3 in the rat anterior hypothalamus

Cited by 11 publications

References 39 publications

Long-term modulation of tyrosine hydroxylase activity and expression by endothelin-1 and -3 in the rat anterior and posterior hypothalamus

Long-term modulation of tyrosine hydroxylase activity and expression by endothelin-1 and -3 in the rat anterior and posterior hypothalamus

Short-term Effects of Endothelins on Tyrosine Hydroxylase Activity and Expression in the Olfactory Bulb of Normotensive Rats

Regulation of the Norepinephrine Transporter by Endothelins

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