Involvement of neutrophils in Chagas disease pathology

Andrade, Micássio Fernandes de; Almeida, Valéria Duarte de; Souza, Lara Michelly Soares de; Paiva, Dayane Carla Costa; Andrade, Cléber de Mesquita; Fernandes, Thales Allyrio Araújo de Medeiros

doi:10.1111/pim.12593

Cited by 12 publications

(9 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, another study showed that inflammatory monocytes facilitate the growth of the parasites in the spleen in visceral leishmaniasis (46). T. cruzi induces neutrophil recruitment to the site of infection, where parasites are phagocytized and eliminated by phagolysosome-mediated lysis through the production of myeloperoxidase and reactive oxygen species (47). We found a significant increase in splenic neutrophils (Ly6cmed Ly6G ϩ cells) in infected miR-155 Ϫ/Ϫ mice, which may be due to higher parasitic load in the spleens in these animals rather than the direct effect of miR-155 knockout on neutrophil phenotypes.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-155 Deficiency Exacerbates Trypanosoma cruzi Infection

et al. 2020

View full text Add to dashboard Cite

Chagas disease, caused by the intracellular protozoan parasite Trypanosoma cruzi, is a public health problem affecting 6 to 8 million people, mainly in Latin America. The role of microRNAs in the pathogenesis of Chagas disease has not been well described. Here, we investigate the role of microRNA-155 (miR-155), a proinflammatory host innate immune regulator responsible for T helper type 1 and type 17 (Th1 and Th17) development and macrophage responses during T. cruzi infection. For this, we compared the survival and parasite growth and distribution in miR-155−/− and wild-type (WT) C57BL/6 mice. The lack of miR-155 caused robust parasite infection and diminished survival of infected mice, while WT mice were resistant to infection. Immunological analysis of infected mice indicated that, in the absence of miR-155, there was decreased interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) production. In addition, we found that there was a significant reduction of CD8-positive (CD8+) T cells, natural killer (NK) cells, and NK-T cells and increased accumulation of neutrophils and inflammatory monocytes in miR-155−/− mice. Collectively, these data indicate that miR-155 is an important immune regulatory molecule critical for the control of T. cruzi infection.

show abstract

Section: Discussionmentioning

confidence: 99%

MicroRNA-155 Deficiency Exacerbates Trypanosoma cruzi Infection

et al. 2020

View full text Add to dashboard Cite

show abstract

“…By improving leukocyte function and the balance between Th1 and Th2 antagonistic phenotypes, exercise-induced metabolic adaptations reinforce parasitic control and attenuates tissue damage and mortality in T. cruzi -infected hosts (Malm, 2004; Gleeson, 2007; Novaes et al ., 2016 a ). While Th1 effectors such as IL-12, IFN- γ , TNF- α and MCP-1 exert protective effects, Th2 molecules such as IL-4, IL-10 and TGF- β attenuate NO biosynthesis and increase the host's susceptibility to T. cruzi infection (Teixeira et al ., 2002; De Andrade et al ., 2018). However, concurrent Th2 and T regulatory (Treg) activation are required to avoid Th1 hyperstimulation, which is harmful to the host as it induces exacerbated inflammatory processes, oxidative stress, cardiomyocytolysis, tissue necrosis and reactive fibrosis (Rassi et al ., 2010; Soares et al ., 2010; Teixeira et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

Could pre-infection exercise training improve the efficacy of specific antiparasitic chemotherapy for Chagas disease?

et al. 2019

View full text Add to dashboard Cite

Considering a potential exercise-drug interaction, we investigated whether exercise training could improve the efficacy of specific antiparasitic chemotherapy in a rodent model of Chagas disease. Wistar rats were randomized into five groups: sedentary and uninfected (CT); sedentary and infected (SI); sedentary, infected and treated (SIT); trained and infected (TI); trained, infected and treated (TIT). After 9-weeks running training, the animals were infected with T. cruzi and followed up for 4 weeks, receiving 100 mg kg−1 day−1 benznidazole. No evidence of myocarditis was observed in CT animals. TI animals exhibited reduced parasitemia, myocarditis, and reactive tissue damage compared to SI animals, in addition to increased IFN-γ, IL-4, IL-10, heart non-protein antioxidant (NPA) levels and glutathione-s transferase activity (P < 0.05). The CT, SIT and TIT groups presented similar reductions in parasitemia, cytokines (IFN-γ, TNF-α, IL-4, IL-10, IL-17 and MCP-1), inflammatory infiltrate, oxidative heart damage and antioxidant enzymes activity compared to SI and TI animals, as well as reduced heart microstructural remodeling (P < 0.05). By modulating heart inflammation and redox metabolism, exercise training exerts a protective effect against T. cruzi infection in rats. However, the antiparasitic and cardioprotective effects of benznidazole chemotherapy are more pronounced, determining similar endpoints in sedentary and trained T. cruzi-infected rats.

show abstract

“…Chagas’ disease is an anthropozoonosis caused by the protozoan Trypanosoma cruzi from the Latin America . Abe and coworkers tested trypanocidal activity of ten withanolides from P .…”

Section: Pharmacological Effectsmentioning

confidence: 99%

Withanolides from the genus Physalis: a review on their phytochemical and pharmacological aspects

Huang

et al. 2019

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

Objectives Withanolides are a group of modified C28 ergostane‐type steroids with a C‐22, C‐26 δ‐lactone side chain or a C‐23, C‐26 γ‐lactone side chain. They enjoy a limited distribution in the plant kingdom and predominantly occur in several genera of Solanaceae. Of which, the genus Physalis is an important resource for this type of natural molecules. The present review aims to comprehensively illustrate the structural characteristics and classification of withanolides, and particularly focus on the progression on phytochemical and pharmacological aspects of withanolides from Physalis ranging from January 2015 to June 2019. Key findings Approximately 351 natural withanolides with novel and unique structures have so far been identified from genus Physalis, mainly isolated from the species of P. angulata and P. peruviana. Withanolides demonstrated diverse biological activity, such as anticancer, anti‐inflammatory, antimicrobial, immunoregulatory, trypanocidal and leishmanicidal activity. Their observed pharmacological functions supported the uses of Physalis species in traditional or folk medicines. Summary Due to their unique structure skeleton and potent bioactivities, withanolides are regarded to be promising drug candidates, particularly for developing anticancer and anti‐inflammatory agents. Further investigations for discovering novel withanolides of genus Physalis, exploiting their pharmacological values and evaluating their potency as therapeutic agents are significant work.

show abstract

Involvement of neutrophils in Chagas disease pathology

Cited by 12 publications

References 77 publications

MicroRNA-155 Deficiency Exacerbates Trypanosoma cruzi Infection

MicroRNA-155 Deficiency Exacerbates Trypanosoma cruzi Infection

Could pre-infection exercise training improve the efficacy of specific antiparasitic chemotherapy for Chagas disease?

Withanolides from the genus Physalis: a review on their phytochemical and pharmacological aspects

Contact Info

Product

Resources

About