Involvement of monoaminergic system in the antidepressant‐like effect of riparin I from Aniba riparia (Nees) Mez (Lauraceae) in mice

Abstract

In past studies conducted by our group, riparin I (rip I) isolated from the green fruit of Aniba riparia presented antianxiety effects in mice, while its analogs rip II and III showed anxiolytic and antidepressant-like actions. This time around, we investigated a possible antidepressant activity of rip I using the forced swimming test (FST) and tail suspension test (TST) as predictive tests for antidepressant activity in rodents. In addition, the involvement of the monoaminergic system in this effect was also assessed. rip I was acutely administered by intraperitoneal (i.p.) and oral (p.o) routes to male mice at doses of 25 and 50 mg/kg. Results showed that rip I at both tested doses and administration routes produced a significant decrease in immobility time in FST and TST. The pretreatment of mice with prazosin (1 mg/kg, i.p., an α₁ -adrenoceptor antagonist), yohimbine (1 mg/kg, i.p., an α₂ -adrenoceptor antagonist), SCH23390 (15 μg/kg, i.p., a dopamine D1 receptor antagonist), sulpiride (50 mg/kg, i.p., a dopamine D2 receptor antagonist), p-chlorophenylalanine (100 mg/kg, an inhibitor of serotonin synthesis) or ritanserin (4 mg/kg, a serotonin 5-HT2(A)/2(C) receptor antagonist) blocked the anti-immobility effects elicited by rip I (50 mg/kg, p.o.) in the FST. Taken together, results indicate that rip I produces significant antidepressant-like activity in the FST and TST, and this effect seems to be dependent on its interaction with noradrenergic, dopaminergic and serotonergic systems.

Thymol reverses depression-like behaviour and upregulates hippocampal BDNF levels in chronic corticosterone-induced depression model in female mice

“…However, the forced swimming and tail suspension tests can produce false‐positive results if the drug tested is a general stimulant. In this case, this hypothesis can be ruled out using the open field test, described first by Hall, that consists of evaluating a series of parameters, including hyperactivity, exploratory behaviour and locomotor activity . The absence of interference in the pattern of square crossings observed in the present study allowed us to conclude that corticosterone, as well as fluvoxamine and thymol, did not interfere in the locomotor activity of the animals.…”

Antidepressant-Like Effect of Lippia sidoides CHAM (Verbenaceae) Essential Oil and Its Major Compound Thymol in Mice

“…Rearing is the animals' ability to stand on its hind legs to explore the environment vertically and it is related to anxiety behaviors [48]. In our protocol, it was observed that neither LSEO nor TML doses were able to change this parameter.…”

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“…When riparin I was tested using the FST and TST, it also decreased the immobility time. Pretreatment with monoamine antagonists likewise demonstrated the implication of these systems in the com-poundʼs effects [71].…”

“…Riparin II (79) [70] and riparin I (78, Fig. 8S, Supplementary Information) [71] were also tested as potential antidepressant-like agents. The former was tested in rodents, with the results showing a significant decrease of immobility time in both the FST and TST.…”