Involvement of miRNAs-mediated senescence and salicylic acid defense in postharvest litchi downy blight

Yin, Chang-Cheng; Xie, Lihong; Wu, Yanrong; Qu, Hongxia; Yang, Bao; Gong, Liang; Jiang, Yueming; Li, Taotao

doi:10.1016/j.foodchem.2022.134662

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…The expression of NPR1, PR1, and PR5 showed an upward trend upon exogenous SA treatment, indicating that H. brassicae activates the defense system of pakchoi and the SA-mediated disease-resistance pathway plays a major role in this defense process. Previous studies reported that the defense response of grapes, canola [21], Arabidopsis [22], cucumber [23], spinach [24], and lychee [25] against DM is predominantly mediated by SA [26]. These results are consistent with our results that SA plays a crucial role in regulating plants' resistance against DM.…”

Section: Discussionsupporting

confidence: 93%

Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew

Chen,

Miao,

et al. 2023

IJMS

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Downy mildew caused by the obligate parasite Hyaloperonospora brassicae is a devastating disease for Brassica species. Infection of Hyaloperonospora brassicae often leads to yellow spots on leaves, which significantly impacts quality and yield of pakchoi. In the present study, we conducted a comparative transcriptome between the resistant and susceptible pakchoi cultivars in response to Hyaloperonospora brassicae infection. A total of 1073 disease-resistance-related differentially expressed genes were identified using a Venn diagram. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these genes were mainly involved in plant−pathogen interaction, plant hormone signal transduction, and other photosynthesis-related metabolic processes. Analysis of the phytohormone content revealed that salicylic acid increased significantly in the resistant material after inoculation with Hyaloperonospora brassicae, whereas the contents of jasmonic acid, abscisic acid, and 1-aminocyclopropane-1-carboxylic acid decreased. Exogenous salicylic acid treatment also significantly upregulated Hyaloperonospora brassicae-induced genes, which further confirmed a crucial role of salicylic acid during pakchoi defense against Hyaloperonospora brassicae. Based on these findings, we suggest that the salicylic-acid-mediated signal transduction contributes to the resistance of pakchoi to downy mildew, and PAL1, ICS1, NPR1, PR1, PR5, WRKY70, WRKY33, CML43, CNGC9, and CDPK15 were involved in this responsive process. Our findings evidently contribute to revealing the molecular mechanism of pakchoi defense against Hyaloperonospora brassicae.

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Section: Discussionsupporting

confidence: 93%

Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew

Chen,

Miao,

et al. 2023

IJMS

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“…Accumulated evidence has highlighted the importance of miRNA in plant-pathogen interactions [42,43]. The role of miRNAs in wheat-pathogen interactions remains largely unknown, and the characterization of resistance-related miRNAs will be helpful for analyzing the regulatory mechanisms of wheat-pathogen interactions and breeding disease-resistant wheat varieties.…”

Section: Discussionmentioning

confidence: 99%

Tae-miR397 Negatively Regulates Wheat Resistance to Blumeria graminis

Guan,

Wei,

Zhou

et al. 2023

Plants

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MicroRNA (miRNA) plays a crucial role in the interactions between plants and pathogens, and identifying disease-related miRNAs could help us understand the mechanisms underlying plant disease pathogenesis and breed resistant varieties. However, the role of miRNA in wheat defense responses remains largely unexplored. The miR397 family is highly conserved in plants and involved in plant development and defense response. Therefore, the purpose of this study was to investigate the function of tae-miR397 in wheat resistance to powdery mildew. The expression pattern analysis revealed that tae-miR397 expression was higher in young leaves than in other tissues and was significantly decreased in wheat Bainong207 leaves after Blumeria graminis (Bgt) infection and chitin treatment. Additionally, the expression of tae-miR397 was significantly down-regulated by salicylic acid and induced under jasmonate treatment. The overexpression of tae-miR397 in common wheat Bainong207 enhanced the wheat’s susceptibility to powdery mildew in the seedling and adult stages. The rate of Bgt spore germination and mycelial growth in transgenic wheat plants overexpressing tae-miR397 was faster than in the untransformed wild-type plants. The target gene of tae-miR397 was predicted to be a wound-induced protein (Tae-WIP), and the function was investigated. We demonstrated that silencing of Tae-WIP via barley-stripe-mosaic-virus-induced gene silencing enhanced wheat’s susceptibility to powdery mildew. qRT-PCR indicated that tae-miR397 regulated wheat immunity by controlling pathogenesis-related gene expressions. Moreover, the transgenic plants overexpressing tae-miR397 exhibited more tillers than the wild-type plants. This work suggests that tae-miR397 is a negative regulator of resistance against powdery mildew and has great potential for breeding disease-resistant cultivars.

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“…Accumulated evidence has highlighted miRNAs' importance in plant-pathogen interactions (Tang et al 2022;Yin et al 2022). Identifying more wheat miRNAs implicated in disease resistance will be helpful to analyze the regulatory mechanism of the wheat-pathogen interaction and breed disease-resistance varieties.…”

Section: Discussionmentioning

confidence: 99%

Tae-miR397 negatively regulates wheat resistance to Blumeria graminis

Guan

Wei

Zhou

et al. 2023

Preprint

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MicroRNA (miRNA) plays a crucial role in the interaction of plant-pathogen, which figures out that exploring more disease-associated miRNAs would aid in our understanding plant disease pathogenesis. However, the role of miRNA in wheat resistance is still largely unknown. In this study, we overexpressed tae-miR397 in wheat to investigate its function in wheat resistance to powdery mildew. The expression pattern analysis revealed that tae-miR397 expression was higher in young leaves than in other tissues, which was significantly decreased in wheat plants after Blumeria graminis (Bgt) infection and chitin treatment. Additionally, the expression of tae-miR397 was significantly down-regulated by salicylic acid and induced under jasmonate treatment. Overexpression of tae-miR397 enhanced wheat sensitivity to powdery mildew. The rate of Bgt spore germination and mycelial growth on transgenic wheat plants overexpressing tae-miR397 was faster than that on untransformed wild type plants. The target gene of tae-miR397 was predicted to be a wound-induced protein (WIP), and its function was investigated. We demonstrated that silencing WIP via barley stripe mosaic virus-induced gene silencing enhanced wheat susceptibility to powdery mildew. Furthermore, we found that tae-miR397 regulated wheat immunity by controlling pathogenesis-related gene expressions. Moreover, transgenic plants overexpressing tae-miR397 exhibited more tillers than wild type plants. These results reveal that tae-miR397 is a negative regulator of resistance against powdery mildew and highlight the role of tae-miR397 in positively regulating the tiller number.

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Involvement of miRNAs-mediated senescence and salicylic acid defense in postharvest litchi downy blight

Cited by 12 publications

References 38 publications

Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew

Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew

Tae-miR397 Negatively Regulates Wheat Resistance to Blumeria graminis

Tae-miR397 negatively regulates wheat resistance to Blumeria graminis

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