Involvement of microRNA‐93, a new regulator of PTEN/Akt signaling pathway, in regulation of chemotherapeutic drug cisplatin chemosensitivity in ovarian cancer cells

Fu, Xin; Tian, Jing; Zhang, Lei; Chen, Ying; Qin, Hao

doi:10.1016/j.febslet.2012.03.006

Cited by 173 publications

(133 citation statements)

References 40 publications

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…Recently, several studies have shown that miRNA played an important role in chemo resistance of ovarian cancer. Mir-199b-5p is associated with acquired chemo resistance in ovarian cancer [6] and miR-93 contributes to cisplatin chemo sensitivity in ovarian cancer cells by regulating PTEN/Akt pathway [7]. Additionally, miR-29b and its signaling mechanism involved in patients with ovarian cancer [8].…”

Section: Introductionmentioning

confidence: 99%

Down‐regulated expression of miR‐134 contributes to paclitaxel resistance in human ovarian cancer cells

et al. 2015

View full text Add to dashboard Cite

a b s t r a c tMiR-134 has been reported to have a role in the development and progression of various cancers. In this study, we found that miR-134 expression was significantly decreased in chemo-resistant serous epithelial ovarian cancer (EOC) patients. Over-expression of miR-134 enhanced the sensitivity of SKOV3-TR30 cells to paclitaxel, and increased paclitaxel-induced apoptosis. Further, Pak2 was identified as a direct target of miR-134, and Pak2-specific siRNA increased cell inhibition rate and promoted paclitaxal-induced apoptosis. By regulating Pak2 expression, miR-134 could mediate Bad phosphorylation at Ser112 and Ser136, which affected cell survival and apoptosis. In conclusion, our findings indicate that repression of miR-134 and consequent up-regulation of Pak2 might contribute to paclitaxel resistance.

show abstract

Section: Introductionmentioning

confidence: 99%

Down‐regulated expression of miR‐134 contributes to paclitaxel resistance in human ovarian cancer cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Xu et al (27) revealed that miR-25 promoted cell migration and invasion in ESCC. In prostate cancer and ovarian cancer, miR-93 can bind to and decrease PTEN expression, thereby promoting cancer cell proliferation and invasion (28,29). These results indicate that certain genes in the PPI network serve important roles in tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

Wang

Yang

Jin³

et al. 2017

Exp Ther Med

View full text Add to dashboard Cite

Abstract. MicroRNAs (miRNAs/miRs) are a class of small, non-coding RNA molecules that serve a key function in carcinogenesis and tumor progression. Recent evidence indicates that miRNAs may act as powerful regulators of migration and invasion. The present study aimed to investigate the effect of miR-25 on the invasion and metastasis of KYSE-150 and EC109 esophageal squamous cell carcinoma (ESCC) cells, and predict the mechanism of this effect by bioinformatically analyzing the miR-106b-25 cluster. In order to alter the expression of miR-25 in the two cell lines, a miR-25 inhibitor or mimic were transfected into the cells, which were then studied via Transwell migration and invasion assays. Subsequently, the target genes of the miR-106b-25 cluster were predicted using miRanda, PicTar, TargetScan and miRTarbase, and the functions of the target genes were predicted via Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Then, a protein-protein interaction (PPI) network was produced using the Search Tool for the Retrieval of Interacting Genes. The results revealed that overexpressing miR-25 led to significantly increased cell migration and invasion in KYSE150 and EC109 cells. Suppressing miR-25 resulted in significantly decreased cell migration and invasion in KYSE150 cells, while the result was not significant in EC109 cells. Target genes of the miR-106b-25 cluster were significantly enriched in the biological process regulation of cellular metabolic process and several cancer-associated pathways, such as those for glioma and melanoma. The PPI network revealed that PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may serve core roles within the network and associate with one another during the pathogenesis of ESCC. These results indicate that a high expression of miR-25 promotes the invasion and metastasis of ESCC cells, while the influence of low expression of miR-25 differs with cells with different degrees of differentiation. Invasion and metastasis are not effected in cells with poor differentiation, while they were decreased in well differentiated cells. Furthermore, PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may be targeted by the miR-106b-25 cluster, and act together to regulate the development of ESCC. IntroductionEsophageal cancer is an important public health problem worldwide, as the eighth most common cancer and sixth most common cause of cancer-associated mortality (1). Esophageal squamous cell carcinoma (ESCC) accounts for 90% of all histological types of esophageal carcinoma in the highest risk area, North-central China, which ranks first in ESCC morbidity and mortality rates (2). Genetic heterogeneity and complex regulatory networks make it difficult to treat ESCC (3). Thus, identifying the regulatory pathways for the stratification of ESCC is of great clinical significance.MicroRNAs (miRNAs/miRs) are single-stranded RNAs of 18-24 nucleotides in length that post-transcriptionally regulate gene expression by directly bindin...

show abstract

“…While no previous findings have characterized miR-183-5p in NPC, our data revealed a significant the correlation between miR-183-5p and the NPC N stages (p<0.05). miR-93-5p has been found to be significantly up-regulated in laryngeal squamous cell carcinoma and in cisplatinresistant ovarian cancer cells, and may promote tumor growth and angiogenesis by targeting integrin-β8 (Fu et al, 2012;Cao et al, 2013). Additionally, a decreased miR-93 expression has been implicated as a prognostic factor for colon cancer (Xiao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Tang¹,

Zhong²,

Li³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.

show abstract

Involvement of microRNA‐93, a new regulator of PTEN/Akt signaling pathway, in regulation of chemotherapeutic drug cisplatin chemosensitivity in ovarian cancer cells

Cited by 173 publications

References 40 publications

Down‐regulated expression of miR‐134 contributes to paclitaxel resistance in human ovarian cancer cells

Down‐regulated expression of miR‐134 contributes to paclitaxel resistance in human ovarian cancer cells

Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Contact Info

Product

Resources

About