Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-signaling regulating the maturation of male accessory glands in the moth Agrotis ipsilon

Gassias, Edmundo; Maria, Annick; Couzi, Philippe; Demondion, Elodie; Durand, Nicolas; Bozzolan, Françoise; Aguilar, Paleo; Debernard, Stéphane

doi:10.1016/j.ibmb.2021.103566

Cited by 15 publications

(25 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are consistent with evidence that Kr-h1 is a JH responsive gene in B. terrestris [48,92]. Our gene knock-down experiments establish Kr-h1 as a pivotal signaling gene acting downstream of JH in bumble bees, consistent with its key roles in JH signaling pathways in other insect species [78,81,[86][87][88]90,[115][116][117][118][119][120].…”

Section: Discussionsupporting

confidence: 90%

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Pandey¹,

Bloch²

2021

Preprint

View full text Add to dashboard Cite

Dominance hierarchies are ubiquitous in invertebrates and vertebrates, but little is known on how genes influence dominance rank. Our gaps in knowledge are specifically significant concerning female hierarchies and in insects. To start filling these gaps we studied the social bumble bee Bombus terrestris, in which social hierarchies among females are common and functionally significant. Dominance rank in this bee is influenced by multiple factors, including juvenile hormone (JH) that is a major gonadotropin in this species. We tested the hypothesis that the JH responsive transcription factor Krüppel homologue 1 (Kr-h1) mediates hormonal influence on dominance behavior in the bumble bee. We first developed and validated a perfluorocarbon nanoparticles-based RNA interference protocol for knocking down Kr-h1 expression. We then used this procedure to show that Kr-h1 mediates the influence of JH not only on oogenesis and wax production, but also on aggression and dominance rank. To the best of our knowledge, this is the first study causally linking a gene to dominance rank in social insects, and one of only a few such studies in insects or in female hierarchies. These findings are important for determining whether there are general molecular principles governing dominance rank across gender and taxa.

show abstract

Section: Discussionsupporting

confidence: 90%

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Pandey¹,

Bloch²

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…These findings are consistent with evidence that Kr-h1 is a JH responsive gene in B. terrestris [47,83]. Our gene knock-down experiments establish Kr-h1 as a pivotal signaling gene that is expressed in multiple tissues and acts downstream of JH in bumble bees, consistent with its key roles in JH signaling pathways in other insect species [82,86,89,[94][95][96][97][132][133][134][135][136]. These findings also set the stage for a deeper study on JH signaling pathways and the genes with which Kr-h1 interacts in bumble bees, as well as other hymenopteran insects.…”

Section: Discussionsupporting

confidence: 89%

Krüppel-homologue 1 Mediates Hormonally Regulated Dominance Rank in a Social Bee

Pandey

Bloch

2021

Biology

View full text Add to dashboard Cite

Dominance hierarchies are ubiquitous in invertebrates and vertebrates, but little is known on how genes influence dominance rank. Our gaps in knowledge are specifically significant concerning female hierarchies, particularly in insects. To start filling these gaps, we studied the social bumble bee Bombus terrestris, in which social hierarchies among females are common and functionally significant. Dominance rank in this bee is influenced by multiple factors, including juvenile hormone (JH) that is a major gonadotropin in this species. We tested the hypothesis that the JH responsive transcription factor Krüppel homologue 1 (Kr-h1) mediates hormonal influences on dominance behavior. We first developed and validated a perfluorocarbon nanoparticles-based RNA interference protocol for knocking down Kr-h1 expression. We then used this procedure to show that Kr-h1 mediates the influence of JH, not only on oogenesis and wax production, but also on aggression and dominance rank. To the best of our knowledge, this is the first study causally linking a gene to dominance rank in social insects, and one of only a few such studies on insects or on female hierarchies. These findings are important for determining whether there are general molecular principles governing dominance rank across gender and taxa.

show abstract

“…Removal of the JH-producing CA induces a co-inhibition of the sex pheromone-triggered orientation flight and the MAG protein synthesis, which can be subsequently restored if operated males are injected with JH ( Anton and Gadenne, 1999 ). The expression levels of Kr-h1 and Met in A. ipsilon males are predominant in the antennal lobe and MAGs, wheretheir amount increased concomitantly with age, in parallel with sex pheromone responses and maturation of the MAGs ( Duportets et al, 2012 ; Gassias et al, 2021 ). RNAi experiments show that males injected with either Met or Kr-h1 dsRNA exhibited a significantly reduced MAG length and protein content ( Gassias et al, 2021 ).…”

Section: Other Biological Functions Of Kr-h1 In In...mentioning

confidence: 96%

“…The expression levels of Kr-h1 and Met in A. ipsilon males are predominant in the antennal lobe and MAGs, wheretheir amount increased concomitantly with age, in parallel with sex pheromone responses and maturation of the MAGs ( Duportets et al, 2012 ; Gassias et al, 2021 ). RNAi experiments show that males injected with either Met or Kr-h1 dsRNA exhibited a significantly reduced MAG length and protein content ( Gassias et al, 2021 ). Thus, Kr-h1 and Met are critical for modulating JH-regulated male reproductive maturation.…”

Section: Other Biological Functions Of Kr-h1 In In...mentioning

confidence: 96%

“…In addition to the roles in insect metamorphosis and reproduction, Kr-h1 is also necessary for many other aspects of insect development, such as neuron fate specification and morphogenesis ( Shi et al, 2007 ; Hewes, 2008 ; Fichelson et al, 2012 ; Marchetti and Tavosanis, 2019 ), social hierarchy and behavioral plasticity ( Whitfield et al, 2003 ; Grozinger and Robinson, 2007 ; Shpigler et al, 2010 ; Gospocic et al, 2021 ), maturation of sexual behavior ( Duportets et al, 2012 ; Gassias et al, 2021 ), embryogenesis ( Fernandez-Nicolas and Belles, 2017 ) and metabolic homeostasis ( Kang et al, 2017 ). Most of these physiological processes are also regulated by JH and 20E signaling.…”

Section: Other Biological Functions Of Kr-h1 In In...mentioning

confidence: 99%

See 1 more Smart Citation

Kr-h1, a Cornerstone Gene in Insect Life History

Zhang²

2022

Front. Physiol.

View full text Add to dashboard Cite

Insect life cycle is coordinated by hormones and their downstream effectors. Krüppel homolog1 (Kr-h1) is one of the crucial effectors which mediates the actions of the two critical hormones of insects, the juvenile hormone (JH) and 20-hydroxyecdysone (20E). It is a transcription factor with a DNA-binding motif of eight C2H2 zinc fingers which is found to be conserved among insect orders. The expression of Kr-h1 is fluctuant during insect development with high abundance in juvenile instars and lower levels in the final instar and pupal stage, and reappearance in adults, which is governed by the coordination of JH, 20E, and miRNAs. The dynamic expression pattern of Kr-h1 is closely linked to its function in the entire life of insects. Over the past several years, accumulating studies have advanced our understanding of the role of Kr-h1 during insect development. It acts as a universal antimetamorphic factor in both hemimetabolous and holometabolous species by directly inhibiting the transcription of 20E signaling genes Broad-Complex (Br-C) and Ecdysone induced protein 93F (E93), and steroidogenic enzyme genes involved in ecdysone biosynthesis. Meanwhile, it promotes vitellogenesis and ovarian development in the majority of studied insects. In addition, Kr-h1 regulates insect behavioral plasticity and caste identity, neuronal morphogenesis, maturation of sexual behavior, as well as embryogenesis and metabolic homeostasis. Hence, Kr-h1 acts as a cornerstone regulator in insect life.

show abstract

Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-signaling regulating the maturation of male accessory glands in the moth Agrotis ipsilon

Cited by 15 publications

References 72 publications

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Krüppel-homologue 1 Mediates Hormonally Regulated Dominance Rank in a Social Bee

Kr-h1, a Cornerstone Gene in Insect Life History

Contact Info

Product

Resources

About