Involvement of LIMK1/2 in actin assembly during mouse embryo development

Duan, Xing; Zhang, Haolin; Wu, Lan‐Lan; Liu, Meng-Yao; Pan, Meng-Hao; Ou, Xiang‐Hong; Sun, Shao‐Chen

doi:10.1080/15384101.2018.1482138

Cited by 8 publications

(8 citation statements)

References 37 publications

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“…Inactivation of LIMKs through silencing with dsRNA or the use of the LIMK inhibitor LIMKi3, which shows a good selectivity within the kinome (see the recent review by Chatterjjee and collaborators [30]), induced the disruption of adherent junctions, resulting in the early termination of development before the blastocyst stage [31]. A similar observation was made in an analysis of early mouse embryogenesis: the use of the LIMKi3 inhibitor caused the failure of early blastomere cleavage, compaction and blastocyst formation [32]. These results point to a crucial role of LIMKs during the early stages of development.…”

Section: Expression Of Limks During Developmentmentioning

confidence: 67%

The Role of LIM Kinases during Development: A Lens to Get a Glimpse of Their Implication in Pathologies

Ribba

Fraboulet

Sadoul

et al. 2022

Cells

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The organization of cell populations within animal tissues is essential for the morphogenesis of organs during development. Cells recognize three-dimensional positions with respect to the whole organism and regulate their cell shape, motility, migration, polarization, growth, differentiation, gene expression and cell death according to extracellular signals. Remodeling of the actin filaments is essential to achieve these cell morphological changes. Cofilin is an important binding protein for these filaments; it increases their elasticity in terms of flexion and torsion and also severs them. The activity of cofilin is spatiotemporally inhibited via phosphorylation by the LIM domain kinases 1 and 2 (LIMK1 and LIMK2). Phylogenetic analysis indicates that the phospho-regulation of cofilin has evolved as a mechanism controlling the reorganization of the actin cytoskeleton during complex multicellular processes, such as those that occur during embryogenesis. In this context, the main objective of this review is to provide an update of the respective role of each of the LIM kinases during embryonic development.

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Section: Expression Of Limks During Developmentmentioning

confidence: 67%

The Role of LIM Kinases during Development: A Lens to Get a Glimpse of Their Implication in Pathologies

Ribba

Fraboulet

Sadoul

et al. 2022

Cells

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“…The weight of the testes in LIMK2−/− mice was significantly reduced to approximately 80% compared to that of control mice (Takahashi et al, 2002). In addition, the inhibition of LIMK1/2 activity in mouse causes the failure of embryo cleavage and blastocyst formation (Duan et al, 2018). Fanconi anemia (FANCA) genes, traditionally known for their essential roles in DNA repair and cytogenetic instability, have been demonstrated to be involved in meiosis and germ cell development.…”

Section: Discussionmentioning

confidence: 99%

Identification of Signatures of Selection by Whole-Genome Resequencing of a Chinese Native Pig

et al. 2020

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Identification of genomic signatures of selection that help reveal genetic mechanisms underlying traits in domesticated pigs is of importance. Anqing six-end-white pig (ASP), a representative of the native breeds in China, has many distinguishing phenotypic characteristics. To identify the genomic signatures of selection of the ASP, wholegenome sequencing of 20 ASPs produced 469.01 Gb of sequence data and more than 26 million single-nucleotide polymorphisms. Combining these data with the available whole genomes of 13 Chinese wild boars, 157 selected regions harboring 48 protein-coding genes were identified by applying the polymorphism levels (θπ) and genetic differentiation (F ST) based cross approaches. The genes found to be positively selected in ASP are involved in crucial biological processes such as coat color (MC1R), salivary secretion (STATH), reproduction (SPIRE2, OSBP2, LIMK2, FANCA, and CABS1), olfactory transduction (OR5K4), and growth (NPY1R, NPY5R, and SELENOM). Our research increased the knowledge of ASP phenotype-related genes and help to improve our understanding of the underlying biological mechanisms and provide valuable genetic resources that enable effective use of pigs in agricultural production.

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“…LIMK acts downstream of Rho-GTPase and is mainly distributed in actin synthesis sites, involved in the regulation of cytoskeletal dynamics and cell proliferation during embryo development [ 14 ]. Previous studies showed that another Rho-GTPase effector, ROCK, which is located in the nucleus and cytoplasm [ 9 ], is required for blastocyst cavity formation and normal ICM differentiation [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…LIMK1/2 regulate spindle organization and F-actin levels by phosphorylation of cofilin during mammalian oocyte maturation [ 13 ]. Inhibition of LIMK1/2 impairs cell division and disrupts actin dynamics during mouse early embryo development [ 14 ]. Although the mouse is a more beneficial experimental animal for developmental biology, different processes during early embryo development such as the timing of zygotic genome activation, the period between 1-cell to blastocyst formation, and gene expression patterns are more suitable for study in pigs.…”

Section: Introductionmentioning

confidence: 99%

LIMK1/2 are required for actin filament and cell junction assembly in porcine embryos developing in vitro

Kwon

Seong

Piao

et al. 2020

Asian-Australas J Anim Sci

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Objective: This study was conducted to investigate the roles of LIM kinases (LIMK1 and LIMK2) during porcine early embryo development. We checked the mRNA expression patterns and localization of LIMK1/2 to evaluate their characterization. We further explored the function of LIMK1/2 in developmental competence and their relationship between actin assembly and cell junction integrity, specifically during the first cleavage and compaction.Methods: Pig ovaries were transferred from a local slaughterhouse within 1 h and cumulus oocyte complexes (COCs) were collected. COCs were matured in in vitro maturation medium in a CO2 incubator. Metaphase II oocytes were activated using an Electro Cell Manipulator 2001 and microinjected to insert LIMK1/2 dsRNA into the cytoplasm. To confirm the roles of LIMK1/2 during compaction and subsequent blastocyst formation, we employed a LIMK inhibitor (LIMKi3).Results: LIMK1/2 was localized in cytoplasm in embryos and co-localized with actin in cell-to-cell boundaries after the morula stage. LIMK1/2 knockdown using LIMK1/2 dsRNA significantly decreased the cleavage rate, compared to the control group. Protein levels of E-cadherin and β-catenin, present in adherens junctions, were reduced at the cell-to-cell boundaries in the LIMK1/2 knockdown embryos. Embryos treated with LIMKi3 at the morula stage failed to undergo compaction and could not develop into blastocysts. Actin intensity at the cortical region was considerably reduced in LIMKi3-treated embryos. LIMKi3-induced decrease in cortical actin levels was attributed to the disruption of adherens junction and tight junction assembly. Phosphorylation of cofilin was also reduced in LIMKi3-treated embryos.Conclusion: The above results suggest that LIMK1/2 is crucial for cleavage and compaction through regulation of actin organization and cell junction assembly.

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Involvement of LIMK1/2 in actin assembly during mouse embryo development

Cited by 8 publications

References 37 publications

The Role of LIM Kinases during Development: A Lens to Get a Glimpse of Their Implication in Pathologies

The Role of LIM Kinases during Development: A Lens to Get a Glimpse of Their Implication in Pathologies

Identification of Signatures of Selection by Whole-Genome Resequencing of a Chinese Native Pig

LIMK1/2 are required for actin filament and cell junction assembly in porcine embryos developing in vitro

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