Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection

Auray, Gaël; Lacroix‐Lamandé, Sonia; Mancassola, Roselyne; Dimier‐Poisson, Isabelle; Laurent, Fabrice

doi:10.1016/j.micinf.2007.01.026

Cited by 44 publications

(39 citation statements)

References 29 publications

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“…In newborn calves, a larger number of immune cells are recruited in the dome epithelium of PPs inoculated with carbon black compared with that of the controls, indicating that carbon black induces similar immune cell responses as infectious particles. A recent study has shown that DCs are progressively recruited at the distal epithelium after Cryptosporidium parvam infection in a murine model (Auray et al 2007). In addition, immature DCs are recruited at the intestinal epithelium with PrP (synthetic peptide) in a concentration-dependent manner (Kaneider et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

et al. 2009

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We have previously reported the early uptake and transport of foreign particles into Peyer's patches (PPs) of newborn and 2-month-old calves and shown that the peak uptake of particles occurs 6 h after inoculation, in addition to site- and size-related effects on particle uptake. We now report the distribution of immune cells within PPs of the distal ileum in newborn and 2-month-old calves inoculated with carbon black. The types of immune cells involved in the early uptake and transport of recombinant mouse prion protein (rMPrP) within PPs of newborn calf were investigated by using monoclonal antibodies CD11c, CD14, CD68, CD172a, and CD21. CD11c(+), CD14(+), CD68(+), CD172a(+), and CD21(+) immune cells were widely distributed in four tissue compartments (villi, dome, interfollicular region, and follicles) of PPs in the distal ileum of newborn and 2-month-old calves, whereas CD11c(+), CD14(+), CD172a(+), and CD21(+) immune cells were more prominently distributed in the dome areas of newborn calves than in 2-month-old calves. Moreover, CD11c(+) and CD14(+) dendritic cells, CD172a(+) and CD68(+) macrophages, and CD21(+) follicular dendritic cells containing rMPrP were primarily observed in the dome and inner follicular regions. The deposition of rMPrP within CD11c(+), CD14(+), CD172a(+), and CD68(+) cells, but not CD21(+) cells, was detected in villous regions. rMPrP-positive immune cells within the interfollicular regions included only CD11c(+) and CD172(+) cells. Although the particles used in this investigation do not include the infectious prion protein, PrP(Sc), our experimental setup provides a useful model for studying immune cells involved in the early uptake and transport of PrP(Sc).

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Section: Discussionmentioning

confidence: 99%

Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

et al. 2009

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“…Oocysts of the Nouzilly isolate (NoI) maintained and kindly gifted by R. Mancassola and M. Naciri (Laboratoire de Pathologie Aviaire, Institut National de Recherche Agronomique, Nouzilly, France) were purified as previously described from feces obtained from experimentally infected calves and stored in a 2.5% K 2 Cr 2 O 7 solution for less than 3 months (6). Oocysts from both isolates were bleached and rinsed in phosphatebuffered saline (PBS) before infection (4,29).…”

Section: Parvum Isolatesmentioning

confidence: 99%

Cryptosporidium parvumIsolate-Dependent Postinfectious Jejunal Hypersensitivity and Mast Cell Accumulation in an Immunocompetent Rat Model

et al. 2009

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Cryptosporidium spp. are a cause of self-limited diarrhea in immunocompetent hosts. In immunocompetent rats, Cryptosporidium parvum infection induced digestive hypersensitivity, a key pathophysiological factor in functional digestive disorders such as irritable bowel syndrome (IBS). In such a rat model, we sought to document whether jejunal hypersensitivity depends on C. parvum isolate and is associated with a mast cell accumulation. Five-day-old rats were orally administered 10 5 oocysts of either Nouzilly (NoI) or Iowa (IoI) C. parvum isolate. NoI-infected rats exhibited the lowest food intake on days 7 and 14 postinfection (p.i.). On day 7 p.i., small intestine villus atrophy, crypt hyperplasia, and inflammatory cell infiltration were prominent in NoI-infected rats, with higher numbers of Cryptosporidium forms than in IoI-infected rats. Compared to uninfected control rats, jejunal intraepithelial lymphocytes (IELs) were increased only in NoI-infected rats on day 14 p.i. On day 50 p.i., jejunal hypersensitivity to distension was found only in NoI-infected rats; this hypersensitivity is associated with activated mast cell accumulation. The number of mast cells in the jejunal lamina propria was increased from day 36 p.i. in NoI-infected rats and only at day 120 p.i. in IoI-infected rats. Our data suggest that both the severity of infection (weight loss, reduced food intake, villus atrophy, and IEL accumulation) and the onset of a jejunal hypersensitivity after infection in association with an activated mast cell accumulation are isolate dependent and related to NoI infection. This cryptosporidiosis rat model is a relevant model for the study of underlying mechanisms of postinfectious IBS-like symptoms.

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“…Functional and quantitative deficiencies, involving both non-specific innate defenses and specific cellular and humoral defenses, have already been described in newborns [2]. The intestinal compartment in neonates is almost devoid of immune cells and this could contribute to the high sensitivity observed in response to intestinal pathogens [36][37][38]. It is therefore of a particular interest to develop strategies for the recruitment of immune cells in neonates and for stimulating their intestinal immune systems to improve their resistance to infection.…”

Section: Discussionmentioning

confidence: 99%

Innate defense regulator peptide synergizes with CpG ODN for enhanced innate intestinal immune responses in neonate piglets

Yang¹,

Mao²,

Zhang³

et al. 2012

International Immunopharmacology

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Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection

Cited by 44 publications

References 29 publications

Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

Cryptosporidium parvumIsolate-Dependent Postinfectious Jejunal Hypersensitivity and Mast Cell Accumulation in an Immunocompetent Rat Model

Innate defense regulator peptide synergizes with CpG ODN for enhanced innate intestinal immune responses in neonate piglets

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