Involvement of integrins, MAPK, and NF-κB in regulation of the shear stress-induced MMP-9 expression in endothelial cells

Sun, Huihui; Changjiang, Li; Chen, Huaiqing; Lin, Hui‐Li; Lv, Huixia; Zhang, Yun; Zhang, Mei

doi:10.1016/j.bbrc.2006.12.002

Cited by 49 publications

(44 citation statements)

References 28 publications

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“…In our previous study, we reported that cordycepin inhibited MMP-1 and -3 expression by suppressing AP-1 activation through MAPK signaling pathway in rheumatoid arthritis synovial fibroblasts (32). Also, we have confirmed that TPA-induced MMP-9 expression was significantly inhibited by the selective inhibitor of ERK1/2 (PD98059) or JNK (SP600125) and partly inhibited by selective inhibitors of the p38 MAPK (SB203580) (data not shown), and previous studies support our data (30,33,34). These findings suggest that cordycepin inhibits TPA-stimulated MMP-9 expression by suppressing MAPK activation.…”

Section: Discussionsupporting

confidence: 88%

Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells

Noh

Youn²,

Jung³

et al. 2009

Int J Mol Med

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Abstract. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in breast cancer cell invasion. NF-κB and AP-1 are known to induce MMP-9 expression. We investigated whether cordycepin, an NF-κB or AP-1 inhibitor, can modulate MMP-9 expression and cell invasion in MCF-7 cells. Toxicity of cordycepin was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MMP-9 expression was determined by real-time PCR, Zymography, and Western blot analysis. AP-1 activation was assayed by electrophoretic mobility shift assay (EMSA). MAPK signaling was evaluated by Western blotting with specific p-ERK, and ERK, p-p38, p38, p-JNK, JNK antibodies. Cordycepin suppressed AP-1 activation, but not NF-κB activation in 12-O-tetradecanoylpho-bol-13-acetate (TPA)-treated MCF-7 cells. Cordycepin inhibits TPA-induced MMP-9 expression and cell invasion by suppressing AP-1 activation. Also, cordycepin suppressed the MAPK signaling pathway. Cordycepin is a potent inhibitor of TPA-induced MMP-9 expression and blocks strongly the ability of AP-1 activation via MAPK signaling pathway in MCF-7 cells.

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Section: Discussionsupporting

confidence: 88%

Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells

Noh

Youn²,

Jung³

et al. 2009

Int J Mol Med

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“…Compound (11) was obtained in 47% yield from intermediate (7) by refluxing with chloro-propylamine in cyclopentyl-methyl ether as the solvent. Finally, compounds (9) and (10) were reacted with an excess of aqueous LiOH, while compound (11) was reacted with aqueous NaOH to produce the corresponding salts.…”

Section: Resultsmentioning

confidence: 99%

“…The action of mitogen-activated protein kinase (MAPK) signalling cascade is one of the earliest events following ligand-receptor binding [5][6][7][8] . Through the activation of diverse signalling pathways, including MAPK, the integrins also regulate a variety of biological processes, including cell differentiation, wound-healing, and apoptosis [9][10][11][12] . The integrin receptors play an important role in cell-cell signalling by mediating interactions between cells and, attachment of cells to the extracellular matrix, thus, helping to define the cellular shape, cell motility regulation, and modulation of cell cycle progressions [13][14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Design, synthesis, and biological evaluation of bifunctional thyrointegrin inhibitors: new anti-angiogenesis analogs

Bridoux

Khan

Chen

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…While the focus in earlier reports was on the interaction of white blood cells to a vessel wall (Schmid-Schoenbein et al 1975), recent studies apply fluid shear to unravel signal transduction pathways in endothelial cells (Sun et al 2007). It has been postulated that fluid shear might also regulate bone cell mechanics (Piekarski and Munro 1977;Cowin et al 1991).…”

Section: Instruments and Methods To Study (Sub-) Cellular And Moleculmentioning

confidence: 99%

Mechanomics and Physicomics in Gravisensing

Loon

2008

Microgravity Sci. Technol

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Sensing gravity by 'non-specialized' cells is still puzzling. We don't know where or by which mechanism such cells sense gravity. These questions in 'gravisensing' are not much different from questions in general mechanobiology. Numerous studies have been reported in this field in the last couple of decades. What are the mechanical properties of a cell? Are there differences in mechanical properties between cell types and if so why? How are forces perceived and transduced to a meaningful biological event. Novel techniques such as optical and magnetic tweezers, atomic force microscopy, magnetophoresis and computer modeling make the field of mechanosensing or perhaps physicomics accessible. A similar approach should also be applied for gravity-related research. This paper addresses the current techniques used in mechanosensing and exemplifies how a cell could sense the relatively weak force of gravity.

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Involvement of integrins, MAPK, and NF-κB in regulation of the shear stress-induced MMP-9 expression in endothelial cells

Cited by 49 publications

References 28 publications

Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells

Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells

Design, synthesis, and biological evaluation of bifunctional thyrointegrin inhibitors: new anti-angiogenesis analogs

Mechanomics and Physicomics in Gravisensing

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