Involvement of integrin alpha V gene expression in human melanoma tumorigenicity.

Abstract

Human melanoma originates in the skin and can lead to widespread metastatic disease. Analysis of melanoma biopsy material has shown that the vitronectin receptor, integrin av#3, is a specific marker of the most malignant cells, i.e., vertically invasive primary lesions or distant metastases (Albelda, S. M.,

The αVβ3/αVβ5 integrin inhibitor cilengitide augments tumor response to melphalan isolated limb perfusion in a sarcoma model

“…aV integrins are involved in processes including attachment, migration, regulation of matrix metalloproteinase and transforming growth factor (TGF)-b activation, angiogenesis and apoptosis. 8,9,[44][45][46][47][48] It is therefore plausible that inhibiting aVintegrin-ligand interaction should reduce tumor progression. As a single agent, cilengitide inhibits growth of brain tumors 23 and has modest activity in recurrent clinical glioblastoma, 36 an activity which appears to be amplified by co-therapy with ionizing radiation.…”

Signaling antibodies complexed with adenovirus circumvent CAR and integrin interactions and improve gene delivery

“…30 Ad2 was radiolabeled with Na 125 I using Iodogen beads (Pierce, Rockford, IL, USA). The ␣v integrin-negative M21-L12 cell line 29 was kindly provided by Dr David Cheresh (TSRI). The SF-9 insect cells (Invitrogen, Carlsbad, CA, USA) were transfected with 3 g plasmid DNA using 15 l Superfect (Qiagen, Valentia, CA, USA) in 200 l DMEM (serum-free) at room temperature for 15 min and then added to fresh cultures of SF-9 cell monolayers (about 90% confluency).…”

“…This complex was then added to M21-L12 human melanoma cells which do not express ␣v integrins 29 but can support efficient virus binding. 6 …”

Coligand effects on the solution stability, biodistribution and metabolism of the 99mTc-labeled cyclic RGDfK tetramer

“…Integrin α v β 3 is a receptor for the extracellular matrix proteins with exposed arginine-glycine-aspartic (RGD) tripeptide sequence [6][7][8][9][10]. Integrin α v β 3 is normally expressed at low levels on epithelial cells and mature endothelial cells; but it is highly expressed on the activated endothelial cells in neovasculature of tumors, including osteosarcomas, glioblastomas, melanomas, lung carcinomas, and breast cancer [5,[11][12][13][14][15][16][17][18]. It has demonstrated that integrin α v β 3 is overexpressed on both endothelial and tumor cells in human breast cancer xenografts [19].…”

“…It has demonstrated that integrin α v β 3 is overexpressed on both endothelial and tumor cells in human breast cancer xenografts [19]. It was also reported that the integrin α v β 3 expression correlates well with tumor progression and invasiveness of melanoma, glioma, ovarian and breast cancers [8][9][10][11][12][13][14][15][16][17][18]. The highly restricted expression of integrin α v β 3 during tumor growth, invasion and metastasis present an interesting molecular target for early detection of rapidly growing and metastatic tumors [19][20][21][22][23].…”