Involvement of IL-1 genes in the cellular responses to carbon nanotube exposure

Arnoldussen, Yke Jildouw; Skogstad, Asbjørn; Skaug, Vidar; Kasem, Mayes; Haugen, Aage; Benker, Nathalie; Weinbruch, Stephan; Apte, Ron N.; Zienolddiny, Shanbeh

doi:10.1016/j.cyto.2015.01.032

Cited by 18 publications

(24 citation statements)

References 60 publications

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“…In number metrics, over half of the fibrous materials in CNT-1 consisted of single fibers, but some fractions are bent or curved single fibers, in addition to small fiber bundles and fibrous clusters. The fibers of CNT-2 are shorter where about 90% of the fibrous material consisted of single and straight fibers (Arnoldussen et al, 2015). Previous studies have shown that long MWCNTs, similar to CNT-1 used here, and asbestos-induced acute inflammation and progressive fibrosis in parietal pleura, is probably due to length-dependent retention at the parietal pleura and that there is initially a more efficient clearance of short MWCNTs from the pleural cavity (Murphy et al, 2011(Murphy et al, , 2013.…”

Section: Discussionmentioning

confidence: 83%

“…The novelty of the present study is that it investigated the importance of IL-1 by using IL1-KO mice to study the effects of MWCNTs compared to crocidolite asbestos. We have previously shown that IL-1 may be important in the response to MWCNTs in vitro (Arnoldussen et al, 2015). Histopathological analysis indicated that IL1-KO mice were less prone to inflammation in the pleura than WT mice as there was a significantly reduced level of proliferation in the connective tissue and leukocyte infiltration.…”

Section: Discussionmentioning

confidence: 94%

“…Our previous characterization of the CNTs showed that CNT-2 consists of 15 Â 10 6 fibrous objects/lg material, CNT-1 of 2.8 Â 10 6 fibrous objects/lg material and UICC Crocidolite of 2.9 Â 10 6 fibrous objects/lg material (Arnoldussen et al, 2015). Whereas the fiber number for CNT-1 and UICC Crocidolite is pretty similar, CNT-2 contains $5 times more.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies show the importance of IL-1 signaling in tissue and cell responses to MWCNTs. We have previously shown that cells with intact IL-1 signaling were much more prone to the toxic effects of the MWCNTs than IL1-KO cells, although differences were observed depending on the type of MWCNT (Arnoldussen et al, 2015).…”

Section: Introductionmentioning

confidence: 96%

“…The materials have been characterized previously and dispersed as described before (Arnoldussen et al, 2015). Main characteristics are shown in Supplementary Table S1 and Supplementary Figure S1.…”

Section: Preparation and Dispersion Of The Test Substances In Dispersmentioning

confidence: 99%

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Inflammation in the pleural cavity following injection of multi-walled carbon nanotubes is dependent on their characteristics and the presence of IL-1 genes

et al. 2018

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(2018) Inflammation in the pleural cavity following injection of multi-walled carbon nanotubes is dependent on their characteristics and the presence of IL-1 genes, Nanotoxicology, 12:6, 522-538, DOI: 10.1080/17435390.2018 Upon inhalation, multi-walled carbon nanotubes (MWCNTs) may reach the subpleura and pleural spaces, and induce pleural inflammation and/or mesothelioma in humans. However, the mechanisms of MWCNT-induced pathology after direct intrapleural injections are still only partly elucidated. In particular, a role of the proinflammatory interleukin-1 (IL-1) cytokines in pleural inflammation has so far not been published. We examined the MWCNT-induced pleural inflammation, gene expression abnormalities, and the modifying role of IL-1a and b cytokines following intrapleural injection of two types of MWCNTs (CNT-1 and CNT-2) compared with crocidolite asbestos in IL-1 wild-type (WT) and IL-1a/b KO (IL1-KO) mice. Histopathological examination of the pleura 28 days post-exposure revealed mesothelial cell hyperplasia, leukocyte infiltration, and fibrosis occurring in the CNT-1 (Mitsui-7)-exposed group. The pleura of these mice also showed the greatest changes in mRNA and miRNA expression levels, closely followed by CNT-2. In addition, the CNT-1-exposed group also presented the greatest infiltrations of leukocytes and proliferation of fibrous tissue. WT mice were more prone to development of sustained inflammation and fibrosis than IL1-KO mice. Prominent differences in genetic and epigenetic changes were also observed between the two genotypes. In conclusion, the fibrotic response to MWCNTs in the pleura depends on the particles' physico-chemical properties and on the presence or absence of the IL-1 genes. Furthermore, we found that CNT-1 was the most potent inducer of inflammatory responses, followed by CNT-2 and crocidolite asbestos. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Preparation and Dispersion Of The Test Substances In Dispersmentioning

confidence: 99%

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Inflammation in the pleural cavity following injection of multi-walled carbon nanotubes is dependent on their characteristics and the presence of IL-1 genes

et al. 2018

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Engineered Nanomaterials and Occupational Allergy

Petrarca

Giampaolo²,

Pedata

et al. 2016

Current Topics in Environmental Health and Preventive Medicine

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Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes

Arnoldussen

Anmarkrud

Skaug

et al. 2016

J. Cell Commun. Signal.

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An increasing amount of products containing engineered nanoparticles is emerging. Among these particles are carbon nanotubes (CNTs) which are of interest for a wide range of industrial and biomedical applications. There have been raised concerns over the effects of CNTs on human health. Some types of CNTs are classified as group 2B carcinogens by the International Agency for Research on Cancer. CNTs may also induce pulmonary inflammatory and fibrotic effects. By utilizing CNTs of different lengths, we investigated the role of the proinflammatory cytokine, interleukin-1 (IL-1) on gap junctional intercellular communication (GJIC) by using IL-1 wild-type (IL1-WT) and IL-1 knock-out (IL1-KO) cells. GJIC decreased equally in both cell types after CNT exposure. Immunofluorescence staining showed Gja1 and Gjb2 in gap junctions and hemichannels for both cell types. Gjb1 and Gjb2 expression was low in IL1-KO cells, which was confirmed by protein analysis. Gja1 was upregulated with both CNTs, whereas Gjb1 was down-regulated by CNT-2 in IL1-WT cells. Connexin mRNA expression was regulated differently by the CNTs. CNT-1 affected Gja1 and Gjb2, whereas CNT-2 had an effect on Gjb1. CNTs negatively affect GJIC through gap junctions independently of the length of CNT and IL-1 status. Furthermore, connexin gene expression was affected by IL-1 at transcriptional and translational levels. As both CNTs used in this study are cytotoxic to the cells and reduce cell survival, we suggest that CNT-induced reduction in GJIC may be important for inhibiting transfer of cell survival signals between cells.Electronic supplementary materialThe online version of this article (doi:10.1007/s12079-016-0323-0) contains supplementary material, which is available to authorized users.

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Involvement of IL-1 genes in the cellular responses to carbon nanotube exposure

Cited by 18 publications

References 60 publications

Inflammation in the pleural cavity following injection of multi-walled carbon nanotubes is dependent on their characteristics and the presence of IL-1 genes

Inflammation in the pleural cavity following injection of multi-walled carbon nanotubes is dependent on their characteristics and the presence of IL-1 genes

Engineered Nanomaterials and Occupational Allergy

Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes

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