Involvement of Growth Hormone and Prolactin in the Induction of Vitellogenin Synthesis in Primary Hepatocyte Culture in the Eel, Anguilla japonica

PTHrP potentiating estradiol-induced vitellogenesis in sea bream (Sparus auratus, L.)

“…Isolated hepatocytes in our hands remained viable for up to at least 8 days (trypan blue exclusion) and remained E2 sensitive, which is in accordance with other studies on this and other species (Flouriot et al, 1993;Islinger et al, 1999;Kwon and Mugiya, 1994;Peyon et al, 1996;Riley et al, 2004;Tollefsen et al, 2003;Yeo and Mugiya, 1997).…”

Structural and expression analysis of hepatic vitellogenin gene during ovarian maturation in Anguilla japonica

“…Although the synergistic effect of co-treatment with SPH and MT induced a stronger hepatic vitellogenic response than SPH or MT alone, it should be noted that crude salmon pituitary homogenate was utilized in this study. In addition to sGTHs, the certain hypophysial components of salmon pituitary extract may involve the pattern of vitellogenesis and steroidogenesis as growth hormone (GH) [56,57]. An in vivo study indicated that GH treatment enhanced E 2 receptor levels in hepatocytes [58].…”

In vitro induction of vitellogenin by estradiol 17 β in isolated hepatocytes of catfish, Clarias gariepinus

“…Primary cultures of the rainbow trout hepatocytes have been successfully carried out using MEM (Maitre et al 1986;Vaillant et al 1988), L-15 (Kwon et al 1993;Mori et al 1998), M199 (Islinger et al 1999) and Dulbecco's modified Eagle's medium nutrient mixture/F12 HAM (DMEM/F12) (Flouriot et al 1997). The hepatocytes of Japanese and European eels have been maintained in L-15 (Kwon and Mugiya, 1994) and WE (Komatsu and Hayashi 1998). The primary hepatocyte cultures of carp, Cyprinus carpio, and tilapia apparently succeeded in DMEM/F12 (Smeets et al 1999) and L-15 (Kim and Takemura 2002).…”