Involvement of Gluconeogenic Pathway in Virulence of <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>

Yang, Wenjian; Liu, Y.; Chen, L.; Qian, Guoliang; Liu, H.; Hu, Baishi; Liu, F.

doi:10.1111/j.1439-0434.2007.01328.x

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…Moreover, several other bacterial and fungal pathogens appear to require either gluconeogenesis or isocitrate lyase, or both, for virulence (9,24,33,46,53,56). It therefore appears that different intracellular pathogens have evolved different strategies for success within phagocytes.…”

Section: Discussionmentioning

confidence: 99%

Salmonella enterica Serovar Typhimurium Mutants Unable To Convert Malate to Pyruvate and Oxaloacetate Are Avirulent and Immunogenic in BALB/c Mice

et al. 2009

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Previously, we showed that the Salmonella enterica serovar Typhimurium SR-11 tricarboxylic acid (TCA) cycle must operate as a complete cycle for full virulence after oral infection of BALB/c mice (M. Tchawa Yimga, M. P. Leatham, J. H. Allen, D. C. Laux, T. Conway, and P. S. Cohen, Infect. Immun. 74:1130-1140, 2006). In the same study, we showed that for full virulence, malate must be converted to both oxaloacetate and pyruvate. Moreover, it was recently demonstrated that blocking conversion of succinyl-coenzyme A to succinate attenuates serovar Typhimurium SR-11 but does not make it avirulent; however, blocking conversion of succinate to fumarate renders it completely avirulent and protective against subsequent oral infection with the virulent serovar Typhimurium SR-11 wild-type strain (R. Mercado-Lubo, E. J. Gauger, M. P. Leatham, T. Conway, and P. S. Cohen, Infect. Immun. 76:1128-1134, 2008). Furthermore, the ability to convert succinate to fumarate appeared to be required only after serovar Typhimurium SR-11 became systemic. In the present study, evidence is presented that serovar Typhimurium SR-11 mutants that cannot convert fumarate to malate or that cannot convert malate to both oxaloacetate and pyruvate are also avirulent and protective in BALB/c mice. These results suggest that in BALB/c mice, the malate that is removed from the TCA cycle in serovar Typhimurium SR-11 for conversion to pyruvate must be replenished by succinate or one of its precursors, e.g., arginine or ornithine, which might be available in mouse phagocytes.

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Section: Discussionmentioning

confidence: 99%

Salmonella enterica Serovar Typhimurium Mutants Unable To Convert Malate to Pyruvate and Oxaloacetate Are Avirulent and Immunogenic in BALB/c Mice

et al. 2009

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Biology and Infection Process of Bacterial and Phytoplasmal Pathogens

2017

Microbial Plant Pathogens

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Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis

Xue

Guo

et al. 2015

J Microbiol.

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To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.

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Involvement of Gluconeogenic Pathway in Virulence of Xanthomonas oryzae pv. oryzae

Cited by 3 publications

References 33 publications

Salmonella enterica Serovar Typhimurium Mutants Unable To Convert Malate to Pyruvate and Oxaloacetate Are Avirulent and Immunogenic in BALB/c Mice

Salmonella enterica Serovar Typhimurium Mutants Unable To Convert Malate to Pyruvate and Oxaloacetate Are Avirulent and Immunogenic in BALB/c Mice

Biology and Infection Process of Bacterial and Phytoplasmal Pathogens

Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis

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