Involvement of galectin-1 in reproduction: past, present and future

Barrientos, Gabriela; Freitag, Nancy; Tirado‐González, Irene; Unverdorben, Laura; Jeschke, Udo; Thijssen, Victor L.; Blois, Sandra M.

doi:10.1093/humupd/dmt040

Cited by 76 publications

(93 citation statements)

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“…Integrins appear to play important roles during pregnancy through their involvement in cell invasion, migration and EMT [27]. Previous studies revealed that extracellular Gal-1 secreted by CAFs enhanced the invasiveness of GC cells by upregulating the expression of β1 integrin [22].…”

Section: Resultsmentioning

confidence: 99%

Galectin-1 from cancer-associated fibroblasts induces epithelial–mesenchymal transition through β1 integrin-mediated upregulation of Gli1 in gastric cancer

Yan¹,

Tang²,

Xiong³

et al. 2016

J Exp Clin Cancer Res

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BackgroundGastric cancer (GC) is characterized by the excessive deposition of extracellular matrix, which is thought to contribute to this tumor’s malignant behavior. Epithelial-mesenchymal transition (EMT) is regarded as a crucial contributing factor to cancer progression. Galectin-1 (Gal-1), a β-galactoside-binding protein abundantly expressed in activated cancer-associated fibroblasts (CAFs), has been reported to be involved in GC progression and metastasis by binding to β1 integrin, which, in turn, can bind to matrix proteins and activate intracellular cascades that mediate EMT. Increasing evidence suggests that abnormal activation of the hedgehog (Hh) signaling pathway enhances GC cell migration and invasion. The purpose of our study is to explore the role of Gal-1 in the GC progression and metastasis as well as the regulatory mechanism.MethodsWe hypothesized that Gal-1 binding to β1 integrin would lead to paracrine signaling between CAFs and GC cells, mediating EMT by upregulating Gli1. Invasion and metastasis effects of the Gal-1 and Gli1 were evaluated using wound healing and invasion assay following transfection with mimics. Additionally, to facilitate the delineation of the role of the Hh signaling in GC, we monitored the expression level of associated proteins. We also evaluated the effects of β1 integrin on these processes. Furthermore, Gal-1 and Gli1 expression in GC patient samples were examined by immunohistochemistry and western blot to determine the correlation between their expression and clinicopathologic characteristics. The Kaplan-Meier method and Cox proportional hazards model were used to analyze the relationship of expression with clinical outcomes.ResultsGal-1 was found to induce EMT, GC cell migration and invasion. Further data showed that Gal-1 up-regulated Gli1 expression. β1 integrin was responsible for Gal-1-induced Gli1 expression and EMT. In clinical GC tissue, it confirmed a positive relationship between Gal-1 and Gli1 expression. Importantly, their high expression is correlated to poor prognosis.ConclusionGal-1 from CAFs binds to a carbohydrate structure in β1 integrin and plays an important role in the development of GC by inducing GC metastasis and EMT through targeting Gli1. This study highlights the potential therapeutic value of Gal-1 for suppression of GC metastasis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13046-016-0449-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Galectin-1 from cancer-associated fibroblasts induces epithelial–mesenchymal transition through β1 integrin-mediated upregulation of Gli1 in gastric cancer

Yan¹,

Tang²,

Xiong³

et al. 2016

J Exp Clin Cancer Res

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“…In the mouse, Yakushina et al, 2015 66 shown that galectin-1 stimulates the differentiation of CD4+ cells into T-regulatory cells then being one of the key molecules involved in immune-tolerance. Probably as the result of the above, low expression in the endometrium has been associated to an increased frequency of early pregnancy failures and miscarriages 67,68 .…”

Section: Molecular Biosystems Accepted Manuscriptmentioning

confidence: 99%

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

et al. 2017

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“…These include the proposals that the death ligand FasL, the negative costimulatory molecule PD-L1, and the tryptophan catabolizing enzyme idoleamine 2,3-dioxygenase, which are all expressed at the maternal-fetal interface would respectively kill, render inactive, or starve infiltrating effector T cells (Baban et al , 2004, Chaouat and Clark, 2001, Taglauer et al , 2009). In contrast, there is mounting evidence that the multifunctional carbohydrate-binding protein Galectin-1 may locally regulate decidual T cell behavior to promote a uterine environment supportive of pregnancy (for review see (Barrientos et al , 2013)). Consistent with this possibility, female mice deficient in Galectin-1 show higher rates of fetal loss in allogeneic but not syngeneic pregnancies (Blois et al , 2007).…”

Section: Regulationmentioning

confidence: 99%

T cell behavior at the maternal-fetal interface

Nancy¹,

Erlebacher²

2014

Int. J. Dev. Biol.

113

105

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Understanding the function of T cells at the maternal-fetal interface remains one of the most difficult problems in reproductive immunology. A great deal of work over the last two decades has led to the view that the T cells that populate the decidua have important roles in both normal and pathological pregnancies, but the exact nature of these roles has remained unclear. Indeed, the old assumption that decidual T cells are uniformly threatening to fetal survival because the placenta is fundamentally an ‘allograft’ has given way to the idea that different T cells subsets contribute in different ways to pregnancy success or failure. Accordingly, some T cells are thought to protect the placenta from immune rejection and facilitate embryo implantation, while others are thought to contribute to pregnancy pathologies such as preeclampsia and spontaneous abortion. Here, we review the current state of information on the behavior of decidual T cells with a focus on both mouse and human studies, and with an emphasis on the many unresolved areas within this overall emerging framework.

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Involvement of galectin-1 in reproduction: past, present and future

Abstract: The involvement of gal-1 in the regulation of different processes during the establishment, development and maintenance of pregnancy could be described as unique. Gal-1 has emerged as an important lectin with major functions in pregnancy.

Cited by 76 publications

References 174 publications

Galectin-1 from cancer-associated fibroblasts induces epithelial–mesenchymal transition through β1 integrin-mediated upregulation of Gli1 in gastric cancer

Galectin-1 from cancer-associated fibroblasts induces epithelial–mesenchymal transition through β1 integrin-mediated upregulation of Gli1 in gastric cancer

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

T cell behavior at the maternal-fetal interface

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