Involvement of fibroblast growth factor 18 in dedifferentiation of cultured human chondrocytes

Yamaoka, Hisayo; Nishizawa, Satoru; Asawa, Yukiyo; Fujihara, Yuko; Ogasawara, Toru; Yamaoka, Kazuko; Nagata, Satoru; Takato, Tsuyoshi; Hoshi, Kazuto

doi:10.1111/j.1365-2184.2009.00655.x

Cited by 24 publications

(20 citation statements)

References 42 publications

(52 reference statements)

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“…Moreover, gain-of-function Fgfr3 mutations expand the pool of proliferative, Sox9-positive chondrocytes and decrease chondrocyte hypertrophy (50,51). Fgf18 also promotes chondrogenesis, chondrocyte proliferation and cartilage regeneration in surgically induced models of osteoarthritis (31,32,43,(52)(53)(54)(55)(56). In clinical trials, intra-articular injections of recombinant Fgf18 resulted in dose-dependent increases in total cartilage content, even though thickness of the central medial femorotibial compartment cartilage was unchanged (57).…”

Section: Discussionmentioning

confidence: 99%

Deletion of the PH-domain and Leucine-rich Repeat Protein Phosphatase 1 (Phlpp1) Increases Fibroblast Growth Factor (Fgf) 18 Expression and Promotes Chondrocyte Proliferation

Bradley

Carpio

Newton

et al. 2015

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Deletion of the PH-domain and Leucine-rich Repeat Protein Phosphatase 1 (Phlpp1) Increases Fibroblast Growth Factor (Fgf) 18 Expression and Promotes Chondrocyte Proliferation

Bradley

Carpio

Newton

et al. 2015

Journal of Biological Chemistry

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“…Neither of them are easily collected, because the collection of the latter has to go through invasive procedures and because that of the former is not always available due to the scarceness of the resource. Even if the required number of cells was successfully collected, there are still problems to be solved, like dedifferentiation in the cultured chondrocytes with repeated passaging (28). Based on these facts, it seems clear that an alternative cell resource is waited.…”

Section: Introductionmentioning

confidence: 99%

Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model

et al. 2013

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The establishment of cartilage regenerative medicine has been an important issue in the clinical field, because cartilage has the poor ability of self-repair. Currently, tissue engineering using autologous chondrocytes has risen, but we should investigate more appropriate cell sources that can be obtained without any quantitative limitation. In this study, we focused on induced pluripotent stem (iPS) cells, in which the ethical hurdle does not seem higher than that of embryonic stem cells. Mouse iPS cells were transplanted into the mouse joint defect model of the knee. Strains of the transplants and hosts were arranged to be either closest (homology 75% in genetic background) or identical (100%). For transplantation, we embedded the iPS cells within the collagen hydrogel in order to obtain the effective administration of the cells into defects, which induced the differentiation of the iPS cells. At 8 weeks of transplantation, although the iPS cells with a 75% homology to the host in the genetic background tended to form teratoma, those of 100% showed a joint regeneration. GFP immunohistochemistry proved that the transplanted iPS cells were responsible for the bone and cartilage repair. Taking these results together, the iPS cells are regarded as a promising cell source for the cartilage tissue engineering.

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“…The cultured chondrocytes we investigated are also capable of producing MMPs [19] or growth factors, such including FGFs and TGF surperfamily [20]. However, the cultured chondrocytes conserve the function that they arrest the cell cycle in response to the stimulation for differentiation [21].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of <i>in vivo</i> migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model

Matsuyama¹,

Fujihara²,

Inaki³

et al. 2013

OJRM

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For regenerative medicine, clarification of in vivo migration of transplanted cells is an important task to secure the safety of transplanted tissue. We had prepared tissue-engineered cartilage consisting of cultured chondrocytes with collagen hydrogel and a biodegradable porous polymer, and we clinically applied it for treatment of craniofacial anomaly. To verify the safety of this tissue-engineered cartilage, we had syngenically transplanted the tissue-engineered cartilage using chondrocytes harvested from EGFP-transgenic mice into subcutaneous pocket of wild type mice, and investigated localizations of transplanted chondrocytes in various organs including cerebrum, lung, liver, spleen, kidney, auricle, gastrocnemius, and femur. After 8 to 24 weeks of the transplantation, accumulation of cartilaginous matrices was observed in tissue-engineered cartilage, while EGFP-positive transplanted chondrocytes were localized in this area. Otherwise, no EGFP was immunohistochemically detected in each organ, suggesting that subcutaneously-transplanted chondrocytes do not migrate to other organs through the circulation. In cartilage tissue engineering using cultured chondrocytes, risk for migration and circulation of transplanted cells seemed negligible, and that ectopic growth of the cells was unlikely to occur, showing that this is safe technique with regard to the in vivo migration of transplanted cells.

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Involvement of fibroblast growth factor 18 in dedifferentiation of cultured human chondrocytes

Abstract: FGF18 seems to play a role in maintenance of chondrocyte properties, although its expression was rather high in dedifferentiated chondrocytes. Upregulation of FGF18 in dedifferentiated chondrocytes implied that it may be a marker of dedifferentiation.

Cited by 24 publications

References 42 publications

Deletion of the PH-domain and Leucine-rich Repeat Protein Phosphatase 1 (Phlpp1) Increases Fibroblast Growth Factor (Fgf) 18 Expression and Promotes Chondrocyte Proliferation

Deletion of the PH-domain and Leucine-rich Repeat Protein Phosphatase 1 (Phlpp1) Increases Fibroblast Growth Factor (Fgf) 18 Expression and Promotes Chondrocyte Proliferation

Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model

Evaluation of <i>in vivo</i> migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model

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Involvement of fibroblast growth factor 18 in dedifferentiation of cultured human chondrocytes

Abstract: FGF18 seems to play a role in maintenance of chondrocyte properties, although its expression was rather high in dedifferentiated chondrocytes. Upregulation of FGF18 in dedifferentiated chondrocytes implied that it may be a marker of dedifferentiation.

Cited by 24 publications

References 42 publications

Deletion of the PH-domain and Leucine-rich Repeat Protein Phosphatase 1 (Phlpp1) Increases Fibroblast Growth Factor (Fgf) 18 Expression and Promotes Chondrocyte Proliferation

Deletion of the PH-domain and Leucine-rich Repeat Protein Phosphatase 1 (Phlpp1) Increases Fibroblast Growth Factor (Fgf) 18 Expression and Promotes Chondrocyte Proliferation

Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model

Evaluation of &lt;i&gt;in vivo&lt;/i&gt; migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model

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Evaluation of <i>in vivo</i> migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model