Involvement of estrogens in the process of sex differentiation in two fish species: The rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus)

Guiguen, Yann; Baroiller, Jean‐François; Ricordel, Marie-José; Iseki, Katia Kiyomi; McMeel, Oonagh Marie; Martín, Samuel; Fostier, Alexis

doi:10.1002/(sici)1098-2795(199910)54:2<154::aid-mrd7>3.0.co;2-5

Cited by 282 publications

(104 citation statements)

References 43 publications

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“…The 15-cycle amplification of the SMART cDNA makes the virtual Northern blotting more sensitive than Northern blotting [27] . Actually we usually get the same results in virtual and real Northern blotting (unpublished data), which is consistent with other reports [28] . To obtain full-length cDNA is another hard task, especially the 5 end.…”

Section: Discussionsupporting

confidence: 92%

Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

Wen

Xie

Liu

et al. 2001

Sci. China Ser. C.-Life Sci.

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A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesis and RACE-PCR. The full-length cDNA is 1870 bp. Its 5 untranslated region is 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of AN-NATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated that the FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen and testis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription. The studies on functional roles of genetic factors and maternal RNAs in oogenesis have been mainly focused on the human being and cultured animals. In fish, however, only a few of recent attempts have been made to identify the genetic factors [1] , but the knowledge about regulative mechanism underlying gene transcription and translation in oogenesis is very little. Recently, along with advances of developmental biology, fish oogenesis has been recognized as an important research field. For instance, Selman et al. investigated different stages of oocyte development in zebrafish (Brachydanio rerio) [2] . Tyler and Sumpter reviewed oocyte growth and development in teleosts [3] . Nagahama et al. initiated the studies on molecular biology of oocyte growth and maturation in fish [4] . However, numerous basic questions in fish oogenesis remain unanswered.

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Section: Discussionsupporting

confidence: 92%

Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

Wen

Xie

Liu

et al. 2001

Sci. China Ser. C.-Life Sci.

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“…Treatment with P450 arom inhibitors have been shown to have the same effect as high temperature inhibition. One hundred percent of all-female populations of rainbow trout, and 73.5% of female tilapia treated with the steroidal aromatase inhibitor 1,4,6-androstatriene-3-17-dione became masculinised (Guigen et al, 1999). A non-steroidal aromatase inhibitor, fadrozole, had a similar effect in masculinising genetic female tilapia (Kwon et al, 2000;Nakamura et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Maintenance of Atlantic salmon (Salmo salar) at elevated temperature inhibits cytochrome P450 aromatase activity in isolated ovarian follicles

Watts

Pankhurst

King

2004

General and Comparative Endocrinology

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Atlantic salmon (Salmo salar) broodstock were transferred from natural (12-16 o C) to controlled temperatures of 14, 18 or 22 o C for 3 months during vitellogenesis. Fertility and survival were significantly reduced in eggs from broodstock held at 22 o C relative to 14 or 18 o C. Endocrine mechanisms were disrupted after only one month at 22 o C, as evidenced by decreased plasma vitellogenin (Vtg) and increased plasma testosterone (T) levels and, at later stages, decreased levels of plasma 17β-estradiol (E 2 ) . In vitro incubations of isolated ovarian follicles were carried out at monthly intervals, with follicles exposed to human chorionic gonadotropin, N, 2-0-dibutyryladenosine 3,5-cyclic monophosphate, and the gonadal steroid precursors 17-hydroxyprogesterone, androstenedione and T. After one month of exposure to controlled temperature, T synthesis was generally enhanced in response to all treatments at all temperatures, but E 2 synthesis was inhibited at 22 o C, suggesting temperature impairment of cytochrome P450 aromatase (P450 arom ) synthesis or activity. The effect became less marked as follicles matured suggesting that temperature sensitivity is stage dependent. The results of this study suggest that the inhibitory effects of elevated temperature on E 2 and Vtg synthesis, and subsequent egg development found in the present and earlier studies, arise at least partly, from temperature modulation of P450 arom .

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“…Studies of sex steroid hormones levels and gene expression of 11b-hydroxylase (enzyme involved in the biosynthesis of the 11b-hydroxytestosterone and 11b-hydroxyandrostenedione) during sex differentiation in tilapia and rainbow trout also demonstrated the importance of these steroids in this process [13,21,22]. Similar studies, based on aromatase gene expression (enzyme involved in the biosynthesis of estradiol) and aromatase inhibition during sex differentiation have proven that endogenous oestrogen are responsible for ovarian differentiation in Nile tilapia [11,23,24], Japanese flounder (Paralichthys olivaceus, [25,26]) and rainbow trout [11, see also 16]. On the other hand, the lack of steroid-producing cells before the onset of sexual differentiation and the inefficiency of aromatase inhibitor to impair the ovarian differentiation in the medaka, strongly suggest that ovarian formation could be sex steroid independent [4,27].…”

Section: A U T H O R ' S P E R S O N a L C O P Ymentioning

confidence: 93%

“…while 11KT (11-oxygenated androgen) is often considered as the main androgen involved in testis differentiation in teleosts [3,[7][8][9][10][11][12]. As testosterone is the hormonal precursor of 11KT and E2, its role in sexual differentiation is therefore of great importance and is generally reflected in the processes of sex differentiation [6,[13][14][15].…”

Section: A U T H O R ' S P E R S O N a L C O P Ymentioning

confidence: 99%

Sex steroid dynamics during embryogenesis and sexual differentiation in Eurasian perch, Perca fluviatilis

et al. 2007

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It is widely accepted that sex steroid hormones play an important and a specific role during the process of sex differentiation in fish. In order to describe the role of the three main sex steroid hormones (testosterone-T, 17b-estradiol-E2 and 11keto-testosterone-11KT) during embryogenesis and sex differentiation in Eurasian perch, Perca fluviatilis, eggs, larvae and juveniles originating from two mixed-sex and two all-female progenies were regularly sampled from fertilization to hatching (D0) and from hatching to day 70 post-hatching (D70). Just after spawning, a significant amount of sex steroids [T (1634.2 pg g ) and significantly increased after the onset of the histological differentiation of the gonad in both progenies (D35). Levels of 11KT were significantly higher in mixed-sex (median: 431.5 pg g À1) than in all-female progenies (below the limit of assay detection) and significantly increased at D35 in all-female progenies (median value: 343.2 pg g À1 ). Mean 11KT to E2 ratio was six-fold higher in mixed-sex progenies (1.35) than in all-female progenies (0.24). The data suggest that the 11-oxygenated androgen (11KT) plays a major role in the male differentiation process, and that sex differentiation in Eurasian perch is probably determined by the 11KT to E2 ratio. #

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Involvement of estrogens in the process of sex differentiation in two fish species: The rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus)

Cited by 282 publications

References 43 publications

Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

Maintenance of Atlantic salmon (Salmo salar) at elevated temperature inhibits cytochrome P450 aromatase activity in isolated ovarian follicles

Sex steroid dynamics during embryogenesis and sexual differentiation in Eurasian perch, Perca fluviatilis

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