Involvement of dual‐strand of the <i>miR‐144</i> duplex and their targets in the pathogenesis of lung squamous cell carcinoma

Uchida, Akifumi; Seki, Naohiko; Mizuno, Keiko; Misono, Shunsuke; Yamada, Yasutaka; Kikkawa, Naoko; Sanada, Hiroki; Kumamoto, Takuma; Shioiri, Takayuki; Inoue, Hiromasa

doi:10.1111/cas.13853

Cited by 30 publications

(35 citation statements)

References 51 publications

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“…The procedures for transfecting miRNAs, siRNAs and plasmid vectors were described in our previous studies [ 20 , 21 , 22 , 23 , 24 ]. Functional assays (cell proliferation and cell cycle) were performed in LUAD cells, as described in our previous studies [ 20 , 21 , 22 , 23 , 24 ]. The reagents used are listed in Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…The vectors used for this analysis were constructed as described in our previous studies [ 20 , 21 , 22 , 23 , 24 ]. Supplementary Figure S1 shows the sequences incorporated into the vectors.…”

Section: Methodsmentioning

confidence: 99%

“…Our recent studies have demonstrated that some passenger strands derived from pre-miRNAs contribute to the malignant transformation of cancer cells [ 15 , 16 , 17 , 18 , 19 ]. We have shown that both strands of miRNAs (e.g., pre- miR-144 , pre- miR-145 and pre- miR-150 ) were significantly downregulated in lung cancer tissues, and their ectopic expression attenuated the malignant phenotypes of lung cancer cells (e.g., cancer cell proliferation, migration and invasion) [ 20 , 21 , 22 , 23 , 24 ]. The involvement of the passenger strand of miRNAs in the pathogenesis of cancer is a new theme in cancer research.…”

Section: Introductionmentioning

confidence: 99%

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FAM64A: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of miR-99a (miR-99a-5p and miR-99a-3p)

Mizuno

Tanigawa

Nohata

et al. 2020

Cells

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Lung adenocarcinoma (LUAD) is the most aggressive cancer and the prognosis of these patients is unfavorable. We revealed that the expression levels of both strands of miR-99a (miR-99a-5p and miR-99a-3p) were significantly suppressed in several cancer tissues. Analyses of large The Cancer Genome Atlas (TCGA) datasets showed that reduced miR-99a-5p or miR-99a-3p expression is associated with worse prognoses in LUAD patients (disease-free survival (DFS): p = 0.1264 and 0.0316; overall survival (OS): p = 0.0176 and 0.0756, respectively). Ectopic expression of these miRNAs attenuated LUAD cell proliferation, suggesting their tumor-suppressive roles. Our in silico analysis revealed 23 putative target genes of pre-miR-99a in LUAD cells. Among these targets, high expressions of 19 genes were associated with worse prognoses in LUAD patients (OS: p < 0.05). Notably, FAM64A was regulated by both miR-99a-5p and miR-99a-3p in LUAD cells, and its aberrant expression was significantly associated with poor prognosis in LUAD patients (OS: p = 0.0175; DFS: p = 0.0276). FAM64A knockdown using siRNAs suggested that elevated FAM64A expression contributes to cancer progression. Aberrant FAM64A expression was detected in LUAD tissues by immunostaining. Taken together, our miRNA-based analysis might be effective for identifying prognostic and therapeutic molecules in LUAD.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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FAM64A: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of miR-99a (miR-99a-5p and miR-99a-3p)

Mizuno

Tanigawa

Nohata

et al. 2020

Cells

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“…The concentrations of miR-144 duplex (miR-144-5p and miR-144-3p) were significantly reduced in squamous NSCLC tissues as compared to healthy adjacent tissues. It was observed that both miR-144-5p and miR-144-3p had tumor inhibitory effects by targeting several oncogenes in squamous NSCLC including neuronal calcium sensor 1 (NCS1), solute carrier family 44 member 5 (SLC44A5), and myristoylated alanine rich protein kinase C substrate (MARCKS) genes [136]. The upregulation of miR-144 enhances LINC00483 and Homeobox A10 (HOXA10) genes in lung adenocarcinoma, resulting in a tumor suppressor activity through the regulation of EMT [137].…”

Section: Mir-144 In Lung Cancermentioning

confidence: 99%

MiR-144: A New Possible Therapeutic Target and Diagnostic/Prognostic Tool in Cancers

Kooshkaki

Rezaei

Rahmati

et al. 2020

IJMS

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MicroRNAs (miRNAs) are small and non-coding RNAs that display aberrant expression in the tissue and plasma of cancer patients when tested in comparison to healthy individuals. In past decades, research data proposed that miRNAs could be diagnostic and prognostic biomarkers in cancer patients. It has been confirmed that miRNAs can act either as oncogenes by silencing tumor inhibitors or as tumor suppressors by targeting oncoproteins. MiR-144s are located in the chromosomal region 17q11.2, which is subject to significant damage in many types of cancers. In this review, we assess the involvement of miR-144s in several cancer types by illustrating the possible target genes that are related to each cancer, and we also briefly describe the clinical applications of miR-144s as a diagnostic and prognostic tool in cancers.Int. J. Mol. Sci. 2020, 21, 2578 2 of 23 of a mRNA molecule [3]. MiRNAs indicate a new perspective in the study of cancers. More than 50% of miRNA genes were discovered to be located within the genomic regions that are involved in cancers, suggesting their role in human cancer pathogenesis. In pathological conditions, MiRNAs can negatively regulate gene expression and they are associated with tumor growth, apoptosis, invasion, and metastasis. In the past, several studies have indicated the ability of miRNAs in the inhibition of tumors as a critical option for the improvement of cancer therapy [4,5]. Tumor activator miRNAs, called oncomiRs, are overexpressed in various cancers. On the contrary, suppressive tumor miRNAs are underexpressed [6][7][8]. Among several miRNAs assessed, miR-144s seem to have a role in several cancers. These miRNAs are located in the chromosomal region 17q11.2, being significantly destroyed in many types of cancers. The predicted stem-loop structure of miR-144s recognized by the miRBase (http://mirbase.org/) is shown in Figure 1A. The miR-144 hairpin gives rise to the "guide strand" miR-144-5p and the sister "passenger" strand miR-144-3p ( Figure 1B).

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“…The concentrations of miR-144 duplex (miR-144-5p and miR-144-3p) were significantly reduced in squamous NSCLC tissues compared to healthy adjacent tissues. It was observed that both miR-144-5p and miR-144-3p had tumor inhibitory effects by targeting several oncogenes in squamous NSCLC including neuronal calcium sensor 1 (NCS1), solute carrier family 44 member 5 (SLC44A5), and myristoylated alanine rich protein kinase C substrate (MARCKS) genes [77]. Upregulation of miR-144 enhance LINC00483 and Homeobox A10 (HOXA10) genes in lung adenocarcinoma resulting in a tumor suppressor activity through regulation of EMT [78].…”

Section: Mir-144 In Lung Cancermentioning

confidence: 99%

MiR-144: A New Possible Therapeutic Target in Cancers

Kooshkaki¹,

Rezaei²,

Rahmati³

et al. 2020

Preprint

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MicroRNAs (miRNAs) are small and non-coding RNAs displaying aberrant expression in the tissue and plasma of cancer patients in comparison to healthy individuals. In past decades, accumulating data proposed that miRNAs could be diagnostic and prognostic biomarkers in cancer patients. It has been identified that miRNAs can act either as oncogenes through silencing of tumor inhibitors or tumor suppressor via targeting oncoproteins. MiR-144 is located in chromosomal region 17q11.2 that was widely destroyed in many types of cancers. Several studies revealed that miR-144 has different target genes including rapamycin, zonula occludens1, SFRP1, and ANO1. MiR-144 acts as a tumor suppressor or oncogene by targeting specific genes. In this review, we define the role of miR‐144 and its targets in different cancers and provide understanding in tumor proliferation, migration, and apoptosis.

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Involvement of dual‐strand of the miR‐144 duplex and their targets in the pathogenesis of lung squamous cell carcinoma

Cited by 30 publications

References 51 publications

FAM64A: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of miR-99a (miR-99a-5p and miR-99a-3p)

FAM64A: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of miR-99a (miR-99a-5p and miR-99a-3p)

MiR-144: A New Possible Therapeutic Target and Diagnostic/Prognostic Tool in Cancers

MiR-144: A New Possible Therapeutic Target in Cancers

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