Involvement of Campylobacter jejuni in septic arthritis: a case report

Colosimo, Manuela; Grancini, Anna; Daprai, Laura; Cimminiello, Aldo Giovanni; Castelli, Cristina; Restelli, Antonella; Gallelli, Luca; Torresani, Erminio

doi:10.1099/jmmcr.0.000095

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…Reactive arthritis is characterized by painful joints as a result from immune associated inflammation after C. jejuni infection (Colosimo et al 2015). Inflammatory bowel disease (IBD) is characterized by gut inflammation that is also triggered by previous infection of C. jejuni or other bacteria species and viruses (Kalischuk and Buret 2010).…”

Section: Clinical Manifestations Of Campylobacteriosis and Complicationsmentioning

confidence: 99%

Characterization of genes differentially regulated after bile acid exposure in Campylobacter jejuni

Minatelli¹,

Yuri²

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Boulin. ¡Muchas gracias por todo! Sin ustedes mi vida aquí no seria la misma. Los quiero muchísimo chicos! E também queria agradecer ao meu grande amigo David Rastelli por me apoiar desde quando eu ainda estudava para o vestibular... Obrigada por tudo bocó! Meu agradecimento especial para o Caetano Luiz Beber, "meu Pequeno". Muito obrigada por sempre estar me incentivando, me ouvindo e me dando força todos os dias desde que nos conhecemos. Você sabe que cheguei até aqui por sua causa! Eu vou sempre ser grata por toda a ajuda e suporte que você me deu até aqui. Te amo! Aproveitando que já estou escrevendo em português, gostaria de agradecer à minha linda família! Mesmo estando longe de mim, vocês sempre estiveram ao meu lado e obviamente em meu coração. Muito obrigada ao meu pai, Antônio Carlos Minateli, por ter sempre me incentivado a estudar desde pequena, por acreditar em mim e por ter me dado uma ótima educação. Vou ser eternamente grata às oportunidades que você me proporcionou. Muito obrigada à minha mami, Emico Imada, você sempre me diz que tem orgulho de mim e me dá forças sempre que necessito. Talvez você não saiba, mas em vários momentos suas palavras me animaram e me deram mais força para continuar. Você é uma mãe maravilhosa, eu não poderia ter uma mãe melhor. E também queria agradecer às minhas irmãs Micheli Mitsue Imada Minatelli, Juliana Yumy Imada Minatelli e Luciana Tiemy Imada Minatelli. Vocês sempre foram as melhores irmãs que alguém pode ter! Vocês sempre me deram forças para continuar, me alegram, me ouvem e sempre me apoiam. Eu amo todos vocês! Muito obrigada! VI VII

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Section: Clinical Manifestations Of Campylobacteriosis and Complicationsmentioning

confidence: 99%

Characterization of genes differentially regulated after bile acid exposure in Campylobacter jejuni

Minatelli¹,

Yuri²

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Quantitative Proteomics Analysis of Global Protein Expression in Campylobacter jejuni Cultured in Sublethal Concentrations of Bile Acids and Varying Temperatures

Masanta¹

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Campylobacter jejuni is the leading cause of diarrhoea among human beings worldwide.Epidemiological investigations have shown that it affects over 500 million people per year.C. jejuni is mainly transmitted to human through consumption of cross contaminated chicken.In most cases, the diarrhoea clears by itself within 3 to 5 days. But it causes a big discomfort in the affected individuals. In addition, it has a huge economic impact due to sick leaves.Because of this, efforts are being put into understanding how C. jejuni interacts with human beings and other hosts. An indepth understanding of how this pathogen interacts with its hosts will lead to development of appropriate diagnosis tools and prevention measures. Bile acids are a major component of the gut fluid in all the hosts of C. jejuni. However, the interaction of C. jejuni and different types of bile acids at human body temperature of 37 o C is poorly understood. Consequently, this study was designed to unearth the proteomic response in C. jejuni reference strain 81-176 to sublethal concentrations of cholic acid (CA), deoxycholic acid (DCA), lithocholic acid (LCA), taurocholic acid (TCA), chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA) and glycocholic acid (GCA). The specific objectives were: (i) to investigate the response in 81-176 to DCA 0.05% at 37 o C for 12h and 24h using both stable isotope labeling with amino acids in cell culture (SILAC) and label-free analysis with sequantial window acquisation of all theoretical mass spectra (SWATH); and determine a suitable quantitative method for the study. (ii) To use the method selected quantitative method to investigate global protein expression in 81-176 in response to sublethal concentrations of CA, LCA, TCA, CDCA, UDCA and GCA cultured at 37 o C for 12h under microaerophilic conditions. (iii) To identify and characterize a currently uncharacterized and widely induced protein (iv) To use label-free analysis with SWATH to investigate protein expression in 81-176 cultured in temperatures of 37 o C (human) and 42 o C (chicken) without bile acids.. Intially, the capability of C. jejuni to adhere and invade Caco-2 cells in the presence of various concentrations of bile acids was investigated using gentamicin protection assay (GPA). The results showed that DCA, CDCA and GCA promoted adherence and invasion in a dose depandant fashion. LCA and UDCA didn't neither promote nor suppress adherence and invasion. Subsequently, IC 50 of each bile acid was obtained. Half of this concentration of each bile acid corresponded to the concentrations that are present in the large intestines of human Microbiology and Genetics, GAUSS) and Prof. Dr. Holger Reichardt (Celluar and Molecular Immunology, UMG) for accepting to be members of my thesis committee and for their helpful suggestions. PD Dr. Andreas E. Zautner for having accepted me to be a Ph.D student in his group and for the support that he offered me throughout the study. Dr. Raimond Lugert, a senior scientist in the Campylobacter Research group for correcting ...

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Involvement of Campylobacter jejuni in septic arthritis: a case report

Cited by 2 publications

References 30 publications

Characterization of genes differentially regulated after bile acid exposure in Campylobacter jejuni

Characterization of genes differentially regulated after bile acid exposure in Campylobacter jejuni

Quantitative Proteomics Analysis of Global Protein Expression in Campylobacter jejuni Cultured in Sublethal Concentrations of Bile Acids and Varying Temperatures

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