Involvement of Calmodulin in 1α,25-Dihydroxyvitamin D3 Stimulation of Store-operated Ca2+ Influx in Skeletal Muscle Cells

Vázquez, Guillermo; Boland, Ana Russo de; Boland, Ricardo

doi:10.1074/jbc.c901008199

Cited by 40 publications

(25 citation statements)

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“…Representative blots from three independent experiments are shown. Quantitative data and statistical analysis are given in Table I. pling of Gq/11 with PLC, has been previously shown to exert effects similar to those of neomycin [Vazquez et al, 1997[Vazquez et al, , 1998]. In addition, preincubation of myotubes with neomycin, followed by treatment with 1,25(OH) 2 D 3 at the same concentration, suppressed the hormoneinduced increase in PKC specific activity in the particulate fraction, as well as the decrease in cytosol (Fig.…”

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confidence: 76%

“…It was previously shown that 1,25(OH) 2 D 3 -induced VDCC-mediated Ca 2ϩ entry into chick muscle cells becomes significantly stimulated after 3 min of hormone exposure [Vazquez and De Boland, 1993], rapidly (30 -60 s) reaching a plateau level. Also, it was recently demonstrated that the fast and transient (1-2 min) Ca 2ϩ increase observed in these cells upon sterol stimulation in Ca 2ϩ -free medium is entirely dependent on PLC activity [Morelli et al, 1996;Vazquez et al, 1997Vazquez et al, , 1998], with the subsequent activation of PKC a consequence of both cytosolic Ca 2ϩ elevation and DAG formation. On these grounds, myoblasts and myotubes were exposed for 5 min to 10 Ϫ9 M 1,25(OH) 2 D 3 , followed by isolation of the cytosolic and particulate fractions and determination of PKC activity using a specific peptide substrate.…”

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confidence: 99%

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Role of protein kinase C in 1,25(OH)2-vitamin D3 modulation of intracellular calcium during development of skeletal muscle cells in culture

Vázquez

Iñón

et al. 2000

J. Cell. Biochem.

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Regulation of muscle cell Ca(2+) metabolism by 1, 25-dihydroxy-vitamin D(3) [1,25(OH)(2)D(3)] is mediated by the classic nuclear mechanism and a fast, nongenomic mode of action that activates signal transduction pathways. The role of individual protein kinase C (PKC) isoforms in the regulation of intracellular Ca(2+) levels ([Ca(2+)](i)) by the hormone was investigated in cultured proliferating (myoblasts) and differentiated (myotubes) chick skeletal muscle cells. 1,25(OH)(2)D(3) (10(-9) M) induced a rapid (30- to 60-s) and sustained (>5-min) increase in [Ca(2+)](i) which was markedly higher in myotubes than in myoblasts. The effect was suppressed by the PKC inhibitor calphostin C. In differentiated cells, PKC activity increased in the particulate fraction and decreased in cytosol to a greater extent than in proliferating cells after 5-min treatment with 1,25(OH)(2)D(3). By Western blot analysis, these changes were correlated to translocation of the PKC alpha isoform from cytosol to the particulate fraction, which was more pronounced in myotubes than in myoblasts. Specific inhibition of PKC alpha activity using antibodies against this isoform decreased the 1, 25(OH)(2)D(3)-induced [Ca(2+)](i) sustained response associated with Ca(2+) influx through voltage-dependent calcium channels. Neomycin, a phospholipase C (PLC) inhibitor, blocked its effects on [Ca(2+)](i), PKC activity, and translocation of PKC alpha. Exposure of myotubes to 1,2-dioleyl-rac-glycerol (1,2-diolein), also increased [Ca(2+)](i), PKC activity, and the amount of PKC alpha associated with the particulate fraction. Changes in [Ca(2+)](i) induced by diolein were inhibited by calphostin C and nifedipine. The results indicate that PKC alpha activation via PLC-catalyzed phosphoinositide hydrolysis is part of the mechanism by which 1, 25(OH)(2)D(3) regulates muscle intracellular Ca(2+) through modulation of the Ca(2+) influx pathway of the Ca(2+) response to the sterol.

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confidence: 76%

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confidence: 99%

Role of protein kinase C in 1,25(OH)2-vitamin D3 modulation of intracellular calcium during development of skeletal muscle cells in culture

Vázquez

Iñón

et al. 2000

J. Cell. Biochem.

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“…Using pharmacological inhibitors, a role for CaMKII in skeletal muscle SOCE (29) and myosin light chain kinase (a Ca 2ϩ -CaM-dependent kinase related to CaMKII (25)) in endothelial SOCE (30) have been postulated. This finding argues that CaMKII modulation of I SOC is a widespread mechanism that is not cell type-specific.…”

Section: Ca 2ϩmentioning

confidence: 99%

Ca2+-Calmodulin-dependent Protein Kinase II Potentiates Store-operated Ca2+ Current

Machaca

2003

Journal of Biological Chemistry

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“…It has been reported that VD3 promotes keratinocyte differentiation by increasing [Ca 2+ ] i via increased expression of phospholipase C (14,20). In addition to this genomic action, VD3 was postulated to act through a membrane-associated receptor to account for the rapid stimulation of Ca 2+ release from intracellular stores and subsequent Ca 2+ entry in skeletal muscle cells (16,19 ] i seems to be associated with similar directional cellular movements. The present study employed G-GECO and an adenoviral vector, and established a method for longterm Ca 2+ imaging in cell culture conditions.…”

Section: Effect Of Vd3 On Camentioning

confidence: 99%

“…Many of the biological actions of VD3 are mediated by the nuclear vitamin D receptor (VDR), a ligand-dependent transcription factor belonging to the superfamily of steroid/thyroid hormone receptors (13). Moreover, VD3 modulates the intracellular Ca 2+ concentration ([Ca 2+ ] i ) in some cell types (14,16,19,20 …”

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Effect of 1,25-dihydroxyvitamin D3 on spontaneous calcium responses in rat dental epithelial SF2 cells revealed by long-term imaging

et al. 2016

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Genetically encoded calcium indicators (GECIs) are suitable for long-term imaging studies. In this study, we employed a highly sensitive GECI, G-GECO, and achieved efficient gene delivery with an adenoviral vector. The adenoviral vector allowed us to express G-GECO in more than 80% of cells. More than 80% of G-GECO-expressing cells showed an ATP-induced increase in fluorescence intensity due to Ca 2+ release from intracellular stores and subsequent Ca 2+ entry. The fluorescence intensity of these cells was increased more than 2-fold by stimulation with 10 μM ATP. We applied long-term imaging (for ~10 h) to monitor Ca 2+ responses in SF2, a rat dental epithelial cell line, in culture conditions. SF2 cells showed intermittent rises in the intracellular Ca 2+ concentration in the presence of 100 nM 1,25-dihydroxyvitamin D 3 . Many of these Ca 2+ responses began at a specific location in the cytoplasm and spread throughout the entire cytoplasm. The combination of efficient gene delivery with an adenoviral vector and long-term imaging with a highly sensitive GECI enabled detection of intermittent Ca 2+ responses that occur only 3-10 times/h/ 100 cells. This method could be useful to study the effects of Ca 2+ responses for regulating longterm processes, such as gene expression, cell migration, and cell division, in many cell types.The hormonally active form of vitamin D 3 , 1,25-dihydroxyvitamin D 3 (VD3), is associated with calcium and phosphorus homeostasis, and maintains bone content through effects on the intestine, bone, kidney, and parathyroid gland (13). In addition to these traditional calcemic activities, VD3 is involved in cell proliferation, differentiation, and immunomodulation (11). Many of the biological actions of VD3 are mediated by the nuclear vitamin D receptor (VDR), a ligand-dependent transcription factor belonging to the superfamily of steroid/thyroid hormone receptors (13). Moreover, VD3 modulates the intracellular Ca 2+ concentration ([Ca 2+

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Involvement of Calmodulin in 1α,25-Dihydroxyvitamin D3 Stimulation of Store-operated Ca2+ Influx in Skeletal Muscle Cells

Cited by 40 publications

References 34 publications

Role of protein kinase C in 1,25(OH)2-vitamin D3 modulation of intracellular calcium during development of skeletal muscle cells in culture

Role of protein kinase C in 1,25(OH)2-vitamin D3 modulation of intracellular calcium during development of skeletal muscle cells in culture

Ca2+-Calmodulin-dependent Protein Kinase II Potentiates Store-operated Ca2+ Current

<b>Effect of 1,25-dihydroxyvitamin D</b><b><sub>3</sub></b><b> on spontaneous calcium responses in rat dental epithelial SF2 cells revealed by long-term </b><b>imaging </b>

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