Involvement of beta3-adrenergic receptors in relaxation mediated by nitric oxide in chicken basilar artery

Wu, Siyuan; Ootawa, Tomoki; Sekio, Ryoya; Smith, Henry; Islam, Md. Zahorul; Uno, Yasuhiro; Shiraishi, Masayuki; Miyamoto, Atsushi

doi:10.1016/j.psj.2023.102633

Cited by 5 publications

(9 citation statements)

References 46 publications

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“…Nitric oxide (NO) was quantified using aorta specimens prepared in accordance with a previously described method [19], with some modifications. Briefly, each thoracic aorta (n = 5 exterminated snakes; total: 1.20 ± 0.05 mg wet tissue per specimen) was cut into 4 mm segments and immersed in a 500 µL tube containing oxygenated physiological saline at 28 • C (pH 7.4).…”

Section: Nitric Oxide Quantification Using Fluorescencementioning

confidence: 99%

Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis)

Ootawa,

Wu,

Sekio

et al. 2023

Animals

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Vasoreactivity is relatively well documented in terrestrial snakes but has previously been investigated in only one semi-arboreal snake species. Consequently, the extent to which vasoreactivity is common across snake taxa or varies by habitat is unclear. The Tokara habu (Protobothrops tokarensis) is a semi-arboreal snake endemic to only two small adjacent Japanese islands, and hence a useful species for further investigation of vasoreactivity. We evaluated responses to known vasoactive substances in thoracic aortas isolated from Tokara habu. Under resting tension, noradrenaline and angiotensin II induced concentration-dependent contraction, but acetylcholine, serotonin (5-hydroxytriptamine; 5-HT), and isoproterenol induced relaxation followed by contraction. Histamine and rattlesnake bradykinin had no effect. Experiments with receptor-specific antagonists suggest that M1 and M3 receptors are involved in the acetylcholine-induced response; 5-HT1, 5-HT2, and 5-HT7 receptors in the serotonin-induced response; and β1 and β2 adrenoceptors in isoproterenol-induced relaxation. This is the first report on such response patterns in snakes (including serotonin- and isoproterenol-induced relaxation). Nitric oxide may be involved in acetylcholine-induced relaxation but not in the responses to serotonin or isoproterenol. In contrast to the uniform vasoreactivity observed in terrestrial snakes, the vasoreactivity of semi-arboreal snakes may be governed by diverse regulatory mechanisms.

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Section: Nitric Oxide Quantification Using Fluorescencementioning

confidence: 99%

Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis)

Ootawa,

Wu,

Sekio

et al. 2023

Animals

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“…The methods applied in the functional study were as described in our previous study in chickens [12]. Briefly, three or four rings, approximately 2 mm wide, were cut from each duck BA.…”

Section: Functional Studymentioning

confidence: 99%

“…It is a vital artery that constantly supplies blood to the hindbrain and a common cerebrovascular feature across vertebrates that contributes to the maintenance of cerebral circulatory volume [9]. Its role in birds appears to be the same as that in mammals, although chickens are the only avian species in which BA reactivity has been studied [10][11][12]. In chickens, the BA endothelium has been identified as a powerful source of spontaneous NO release, which was based on strong contractions noted after the application of N ω -nitro-L-arginine (L-NNA), a NO synthase (NOS) inhibitor [11].…”

Section: Introductionmentioning

confidence: 99%

“…In chickens, the BA endothelium has been identified as a powerful source of spontaneous NO release, which was based on strong contractions noted after the application of N ω -nitro-L-arginine (L-NNA), a NO synthase (NOS) inhibitor [11]. Furthermore, previous investigations of endothelial cell receptors have indicated that beta-3 adrenergic receptors (β 3 -ARs), histamine (His) H 1 receptors, and muscarinic acetylcholine (ACh) M 3 receptors are involved in the relaxation of the chicken BA via the NO pathway, suggesting this arterial relaxation is strongly dependent on the endothelial release of NO [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation

Wu,

Ootawa,

Sekio

et al. 2023

Animals

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The basilar arterial endothelium mediates blood vessel relaxation partly through the release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to a high mortality rate in chickens infected with the highly pathogenic avian influenza virus, but interestingly, ducks exhibit a greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated with 5-HT1 and H1 receptors, while relaxation is induced with β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated with M3 and OK receptors but not β3-adrenergic receptors in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain the differences in lethality between ducks and chickens when vascular endothelial cells are infected with the virus.

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“…We previously reported differences in vasoreactivity in animals with disease [ 16 ] and deficient nutrition [ 14 , 26 ], based on the responses to endogenous vasoactive substances such as angiotensin (Ang) II, acetylcholine (ACh), noradrenaline (NA) and 5-hydroxytryptamine (5-HT). Furthermore, differences in vasoreactivity between animal species have been reported in reptiles, birds, and mammals [ 15 , 17 , 25 , 27 , 41 , 42 ]. These studies indicate that vasoreactivity is substantially identical within the same animal species, unless it is affected by factors such as disease, nutrition, or environment.…”

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confidence: 99%

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

OOTAWA,

WU,

SEKIO

et al. 2024

The Journal of Veterinary Medical Science

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Habu snakes ( Protobothrops flavoviridis ) are pit vipers found in the geographically adjacent but ecologically divergent islands of Tokunoshima and Amami-Oshima in southwestern Japan. Abiotic factors can cause variation in animal populations between the two islands, and Habu snakes may show such intraspecific physiological variation. We therefore evaluated the vasoreactivity in aortas isolated from the Habu of both islands. Tokunoshima Habu showed significantly greater contractile responses to angiotensin (Ang) II, acetylcholine (ACh) and noradrenaline, and significantly higher affinities (pEC 50 ) for Ang II and ACh, than Amami-Oshima Habu. ACh caused contractions in aortas from both populations, a finding previously unreported in snakes. Our findings indicate that vasoreactivity may differ between Tokunoshima and Amami-Oshima Habu.

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Involvement of beta3-adrenergic receptors in relaxation mediated by nitric oxide in chicken basilar artery

Cited by 5 publications

References 46 publications

Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis)

Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis)

Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

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