Involvement of BDNF and NGF in the Mechanism of Neuroprotective Effect of Human Recombinant Erythropoietin Nanoforms

Abstract

Human recombinant erythropoietin adsorbed on poly(butyl)cyanoacrylate nanoparticles and administered intraperitoneally in a dose of 0.05 mg/kg exhibited a neuroprotective effect in experimental intracerebral posttraumatic hematomas (hemorrhagic stroke) and reduced animal mortality. Human recombinant erythropoietin, native and adsorbed on lactic and glycolic acid copolymer-based nanoparticles, exhibited no antistroke effect on this model. Analysis of reverse transcription PCR products showed that human recombinant erythropoietin adsorbed on poly(butyl)cyanoacrylate nanoparticles more than 2-fold increased the expression of BDNF and NGF neurotrophins in the rat brain frontal cortex and hippocampus.

Growth Factors for the Treatment of Ischemic Brain Injury (Growth Factor Treatment)

“…Because many of these growth factors have common intracellular pathway targets, exogenous administration can lead to altered expression of endogenously produced factors. For example, one hemorrhagic stroke study showed that infusion of nano-particle bound rEPO led to more than a two-fold increase in BDNF and NGF expression [ 88 ]. Further pre-clinical testing is necessary to optimize combination therapy.…”

Transplantation of neurotrophin-3-transfected bone marrow mesenchymal stem cells for the repair of spinal cord injury

“…Additionally, it promotes the formation of neuromuscular junctions and axonal outgrowth (Fan et al, 2010;Yang et al, 2010). Many studies have shown NT-3 can maintain the survival of sensory, motor and sympathetic neurons (Boyce et al, 2012;Thomson et al, 2012;Solev et al, 2013). Treatment with NT-3 has been shown to enhance the sprouting of supraspinal tract axons when injected into the lesion immediately after spinal cord injury Zhang et al, 2013).…”

Stimulating brain recovery after stroke using theranostic albumin nanocarriers loaded with nerve growth factor in combination therapy

“…Today, stroke is a leading cause of death globally and is responsible for 9% of all deaths around the world [2]. A family of neurotrophins including NGF is involved in cerebral recovery [38] and was studied as a therapeutic option for brain injury [7].…”