Involvement of an SRF-MADS protein McmA in regulation of extracellular enzyme production and asexual/sexual development in <i>Aspergillus nidulans</i>

Li, Nuo; Kunitake, Emi; Endo, Y; Aoyama, Miki; Kanamaru, Kyoko; Kimura, Makoto; Kato, Masashi; Kobayashi, Tetsuo

doi:10.1080/09168451.2016.1146074

Cited by 10 publications

(5 citation statements)

References 43 publications

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“…In S. macrospora, as shown here in P. anserina, MCM1 controls different development processes and is essential for the formation of perithecia [47,48]. A similar role has been evidenced in A. nidulans [49], Fusarium graminearum [50], and Colletotrichun fructicola [51]. In the phytopathogen M. oryzae, the gene MoMCM1 was shown to be required for appressorial penetration and male fertility, but it does seem to be involved in fruiting body formation [52].…”

Section: Discussionsupporting

confidence: 63%

Mutations in Podospora anserina MCM1 and VelC Trigger Spontaneous Development of Barren Fruiting Bodies

Essadik,

Boucher,

Bobée

et al. 2024

JoF

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The ascomycete Podospora anserina is a heterothallic filamentous fungus found mainly on herbivore dung. It is commonly used in laboratories as a model system, and its complete life cycle lasting eight days is well mastered in vitro. The main objective of our team is to understand better the global process of fruiting body development, named perithecia, induced normally in this species by fertilization. Three allelic mutants, named pfd3, pfd9, and pfd23 (for “promoting fruiting body development”) obtained by UV mutagenesis, were selected in view of their abilities to promote barren perithecium development without fertilization. By complete genome sequencing of pfd3 and pfd9, and mutant complementation, we identified point mutations in the mcm1 gene as responsible for spontaneous perithecium development. MCM1 proteins are MADS box transcription factors that control diverse developmental processes in plants, metazoans, and fungi. We also identified using the same methods a mutation in the VelC gene as responsible for spontaneous perithecium development in the vacua mutant. The VelC protein belongs to the velvet family of regulators involved in the control of development and secondary metabolite production. A key role of MCM1 and VelC in coordinating the development of P. anserina perithecia with gamete formation and fertilization is highlighted.

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Section: Discussionsupporting

confidence: 63%

Mutations in Podospora anserina MCM1 and VelC Trigger Spontaneous Development of Barren Fruiting Bodies

Essadik,

Boucher,

Bobée

et al. 2024

JoF

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“…). Additional hemicellulase genes show low level ClrB‐ and McmA‐dependent expression based on previously reported genome‐wide transcriptomic analyses (Coradetti et al ., ; Li et al ., ). Nevertheless, mutation of mcmA does not affect the production of mannanolytic and xylanolytic enzymes when cells are grown on glucomannan and xylan (Yamakawa et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…We previously reported that McmA regulates extracellular enzyme production, including cellulases and asexual/sexual development in A. nidulans , and that it binds to the CeRE of the eglA promoter (Yamakawa et al ., ; Li et al ., ). McmA is a member of the SRF‐type MADS box protein family conserved among eukaryotic organisms (Shore and Sharrocks, ).…”

Section: Introductionmentioning

confidence: 97%

“…These factors are not essential for cellulose-induced expression of the cellulase genes, but rather modulate the expression level of the genes in response to intracellular or environmental conditions. We previously reported that McmA regulates extracellular enzyme production, including cellulases and asexual/ sexual development in A. nidulans, and that it binds to the CeRE of the eglA promoter (Yamakawa et al, 2013;Li et al, 2016). McmA is a member of the SRF-type MADS box protein family conserved among eukaryotic organisms (Shore and Sharrocks, 1995).…”

Section: Introductionmentioning

confidence: 99%

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McmA‐dependent and ‐independent regulatory systems governing expression of ClrB‐regulated cellulase and hemicellulase genes in Aspergillus nidulans

Kunitake

Aoyama

et al. 2016

Molecular Microbiology

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Fungal cellulolytic and hemicellulolytic enzymes are promising tools for industrial hydrolysis of cellulosic biomass; however, the regulatory network underlying their production is not well understood. The recent discovery of the transcriptional activators ClrB and McmA in Aspergillus nidulans implied a novel regulatory mechanism driven by their interaction, experimental evidence for which was obtained from transcriptional and DNA-binding analyses in this study. It was found that ClrB was essential for induced expression of all the genes examined in this study, while McmA dependency of their expression was gene-dependent. DNA-binding studies revealed McmA assisted in the recruitment of ClrB to the cellulose-responsive element (CeRE) in the promoters of eglA and eglB, expression of which was significantly reduced in the mcmA mutant. The CCG triplet within the CeRE served as the recognition sequence for the ClrB monomer. In contrast, ClrB did not require McmA for binding as a homodimer to the CGGN CCG sequences in the promoter of mndB, expression of which was affected less in the mcmA mutant than in all other examined genes. Thus, there are two types of ClrB-mediated regulation: McmA-assisted and McmA-independent. This novel McmA-ClrB synergistic system provides new insights into the complex regulatory network involved in cellulase and hemicellulase production.

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“…This could be explained by the fact that N. crassa CLR-2 is fully competent to drive transcription of its targets under noninducing conditions, while Aspergillus ClrB requires other factors, such as XlnR [51, 122], ClbR [17], or McmA [123, 124]. It suggests diversity in the signaling pathways that activate cellulases through XlnR and ClrB in filamentous fungi [98].…”

Section: Transcription Factors Directly Involved In Plant Biomass Degmentioning

confidence: 99%

Regulators of plant biomass degradation in ascomycetous fungi

Benocci

Pontes

Zhou

et al. 2017

Biotechnol Biofuels

179

158

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Fungi play a major role in the global carbon cycle because of their ability to utilize plant biomass (polysaccharides, proteins, and lignin) as carbon source. Due to the complexity and heterogenic composition of plant biomass, fungi need to produce a broad range of degrading enzymes, matching the composition of (part of) the prevalent substrate. This process is dependent on a network of regulators that not only control the extracellular enzymes that degrade the biomass, but also the metabolic pathways needed to metabolize the resulting monomers. This review will summarize the current knowledge on regulation of plant biomass utilization in fungi and compare the differences between fungal species, focusing in particular on the presence or absence of the regulators involved in this process.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0841-x) contains supplementary material, which is available to authorized users.

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Involvement of an SRF-MADS protein McmA in regulation of extracellular enzyme production and asexual/sexual development in Aspergillus nidulans

Cited by 10 publications

References 43 publications

Mutations in Podospora anserina MCM1 and VelC Trigger Spontaneous Development of Barren Fruiting Bodies

Mutations in Podospora anserina MCM1 and VelC Trigger Spontaneous Development of Barren Fruiting Bodies

McmA‐dependent and ‐independent regulatory systems governing expression of ClrB‐regulated cellulase and hemicellulase genes in Aspergillus nidulans

Regulators of plant biomass degradation in ascomycetous fungi

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