Involvement of an l-amino acid oxidase in the activity of the marine bacterium Pseudoalteromonas flavipulchra against methicillin-resistant Staphylococcus aureus

Chen, Wen Ming; Lin, Chang Yi; Chen, Chaolun Allen; Wang, Jih Terng; Sheu, Shih Yi

doi:10.1016/j.enzmictec.2010.03.008

Cited by 27 publications

(15 citation statements)

References 48 publications

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“…The antibacterial activities of PfaP were detected by nondenaturing SDS-PAGE without heat treatment, similar to those of marinocine (Lucas-Elio et al, 2005) and the antibacterial protein of P. flavipulchra C2 (Chen et al, 2010). However, when the ECP sample from JG1 was subjected to PAGE, no inhibition zone formed around the protein bands (results not shown).…”

Section: Structure and Antibacterial Activities Of The Smallmolecule mentioning

confidence: 78%

“…It has been reported that several marine bacterial strains belonging to the Gammaproteobacteria can synthesize antibacterial proteins, including the L-lysine oxidase AlpP of P. tunicata D2 (James et al, 1996), marinocine of Marinomonas mediterranea (Lucas-Elio et al, 2005) and an antibacterial protein of P. flavipulchra C2 (Chen et al, 2010). The molecular masses and antibacterial activities of these proteins were different from each other.…”

Section: Structure and Antibacterial Activities Of The Smallmolecule mentioning

confidence: 99%

“…One lane slice was stained with Coomassie brilliant blue. The other lane slice was washed three times in distilled water and then placed on a MA plate, after which an overnight broth culture of V. anguillarum inoculated into semi-solid MA medium was spread over the gel surfaces in order to examine the antibacterial activity of proteins (Chen et al, 2010). The plate was incubated at 28 uC for 24 h to observe the inhibition zone.…”

Section: Methodsmentioning

confidence: 99%

“…The gel band containing the antibacterial protein was sliced, washed and digested following the method described by Chen et al (2010). The pooled peptide extracts were marked with 4-sulfophenyl isothiocyanate (SPITC), desalted with a C18 Zip-Tip (Millipore) and analysed by a MALDI TOF/TOF mass spectrometer (Bruker, Ultraflex).…”

Section: Methodsmentioning

confidence: 99%

“…P. flavipulchra strain C2, which was isolated from the encrusting pore coral Montipora aequituberculata from the Kenting coast of Southern Taiwan, was found to exhibit antibacterial activity against several meticillin-resistant S. aureus strains and to synthesize an antibacterial protein (Chen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of antibacterial compounds of Pseudoalteromonas flavipulchra JG1

Wang

Tang

et al. 2012

Microbiology

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Pseudoalteromonas flavipulchra JG1 produces a protein PfaP and a range of small-molecule compounds with inhibitory activities against Vibrio anguillarum. The PfaP protein was purified from the extracellular products of JG1 by electroelution, and antibacterial activity was observed by an in-gel antibacterial assay. The complete amino acid sequence (694 aa) of PfaP was determined by de novo peptide sequencing and subsequent alignment with the proteome sequence of strain JG1. The calculated molecular mass of PfaP was 77.0 kDa. PfaP was 58 % identical to L-lysine oxidase AlpP of Pseudoalteromonas tunicata D2, and 54 % identical to the marinocine antimicrobial protein of Marinomonas mediterranea MMB-1. Five small molecules (compounds 1-5) with antibacterial activity, which were identified as p-hydroxybenzoic acid (1), trans-cinnamic acid (2), 6-bromoindolyl-3-acetic acid (3), N-hydroxybenzoisoxazolone (4) and 29-deoxyadenosine (5), were purified by sequential column chromatography over silica gel, Sephadex LH-20 and RP-18 from ethyl acetate extract of strain JG1, and their structures were determined by NMR and MS. Brown compound 3, the only brominated compound, showed antibacterial activity against both Gram-positive and Gram-negative bacteria.

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Section: Structure and Antibacterial Activities Of The Smallmolecule mentioning

confidence: 78%

Section: Structure and Antibacterial Activities Of The Smallmolecule mentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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