Involvement of alkylhydroxybenzenes, microbial autoregulators, in controlling the expression of stress regulons

Golod, N. A.; Лойко, Н. Г.; Lobanov, K. V.; Mironov, A. S.; Voieikova, T. A.; Гальченко, В. Ф.; Nikolaev, Yu. A.; Эль-Регистан, Г. И.

doi:10.1134/s0026261709060034

Cited by 14 publications

(25 citation statements)

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“…Taking account of the earlier established capacity of one of the AHB homologues, C12 AHB, to activate stress gene expression at a sublethal UV irradiation [6,17], we assessed similar regulatory properties in a rep resentative array of autoregulatory factors. Research on AHB effects on the luminescence, viability, and phase variation enabled us to reveal a number of inter linked processes that are associated with the peculiar ities of the AHB chemical structure (Table 1) and their concentrations ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Initial research on alkylhydroxybenzene effects on repair activity suggested the possibility that the stress genes of the bacterial SOS system were subject to both positive and negative regulation, due to the involve ment of AHB homologues with different hydropho bicity [6]. However, cellular protective systems use also a "passive" mechanism involving AHBs as (i) traps for reactive oxygen species (ROS) [10] and (ii) biopolymer molecule stabilizers [7][8][9][10][11].…”

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confidence: 99%

“…Another aspect of the biological activity of AHBs is their functioning as adaptogens involved in developing of microbial resistance to a wide variety of deleterious factors [3]. In a number of studies, the protective effects of AHBs on the cells of bacterial and yeast cul tures under various kinds of stress [4,5], including UV irradiation [6], were demonstrated. This provided the foundation for more detailed research on the mecha nisms of action of the above group of microbial auto regulators.…”

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Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

et al. 2012

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This work is concerned with the role of alkylhydroxybenzenes (AHBs), chemical analogs of the autoregulatory microbial d 1 factors, on the development of the stress response of bacterial cells to UV irradi ation, including SOS system induction, preservation of cell viability, and S R phase transitions of the Escherichia coli test strain with the bioluminescence genes cloned under the control of the recA gene pro moter. UV irradiation, a natural stress factor, and an increase in AHB concentrations were found to elicit uni form responses in bacteria, indicating that AHBs function as alarmones, i.e., alarm signals. It was revealed that preincubating bacteria with alkylhydroxybenzenes considerably enhanced their viability upon irradiation with lethal UV doses; this was accompanied by a relative decrease in the SOS response activity and a concom itant increase in the frequency of phase transitions. The efficiency of the protective action of AHBs increased with an increase in their hydrophobicity degree. The probable mechanism of the protective effect of AHBs is discussed, based on their capacity for the interaction with biopolymers, which results in changing their struc tural organization and conferring resistance to a broad spectrum of stress factors. Such a "passive" protective mechanism reduces the susceptibility of DNA to UV irradiation, causing a decrease in the parameters related to the SOS system induction that is responsible for the "active" protective mechanism in bacterial cells. As a result, viability retention under the lethal influence of UV irradiation is possible at minimal values of repair activity and is accompanied by an increase in the phenotypic variability of the surviving part of a bacterial population.

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Section: Resultsmentioning

confidence: 99%

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Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

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“…Finally, based on these data calculation of the relative index F i allowed to reveal poorly expressed induction/inhibition of SOSsystem as a result of AR exposure (Fig.5C). So, these factors at a concentration of 10 -5 M, leading to the induction of stress response of bacterial cells [Golod et al, 2009], accompanied by the little activation of E.coli recA'::lux relative bioluminescence intensity. On the other hand, a high 10 -3 M concentration, leading to the formation of the bacterial cell anabiotic state [Suzina et al, 2006], caused intensive SOS-response depression.…”

Section: Bacterial Cells Uv Protection By Armentioning

confidence: 99%

“…Potential effects of AR in this system are defined by their ability to absorb ultraviolet radiation in the range of 245-295 nm for different AR homologues, and to act as antioxidant similar to other photoprotectors [Fraikin et al, 2000]. According to accumulated data, AR are also capable to change the activity of stress regulons, including the SOS-response genes [Golod et al, 2009], that are realize an active DNA reparation at various damages.…”

Section: Introductionmentioning

confidence: 99%