Involvement of aldehyde oxidase in the metabolism of aromatic and aliphatic aldehyde-odorants in the mouse olfactory epithelium

Takaoka, Naoki; Sanoh, Seigo; Ohta, Shigeru; Esmaeeli, Mariam; Leimkühler, Silke; Kurosaki, Mami; Terao, Mineko; Garattini, Enrico; Kotake, Yaichiro

doi:10.1016/j.abb.2021.109099

Cited by 3 publications

(4 citation statements)

References 37 publications

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“…For instance, their activity has been demonstrated in the buccal cavity at the salivary [25][26][27] and epithelial levels 27,28 . At the olfactory level, they were also shown to be expressed in mouse sensory cilia 18 , rodent olfactory mucus and epithelium 16,19 and human nasal mucus 10,18,29 .…”

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confidence: 98%

Characterization of human oxidoreductases involved in aldehyde odorant metabolism

Boichot

Ménétrier

Saliou

et al. 2023

Sci Rep

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Oxidoreductases are major enzymes of xenobiotic metabolism. Consequently, they are essential in the chemoprotection of the human body. Many xenobiotic metabolism enzymes have been shown to be involved in chemosensory tissue protection. Among them, some were additionally shown to be involved in chemosensory perception, acting in signal termination as well as in the generation of metabolites that change the activation pattern of chemosensory receptors. Oxidoreductases, especially aldehyde dehydrogenases and aldo–keto reductases, are the first barrier against aldehyde compounds, which include numerous odorants. Using a mass spectrometry approach, we characterized the most highly expressed members of these families in the human nasal mucus sampled in the olfactory vicinity. Their expression was also demonstrated using immunohistochemistry in human epitheliums sampled in the olfactory vicinity. Recombinant enzymes corresponding to three highly expressed human oxidoreductases (ALDH1A1, ALDH3A1, AKR1B10) were used to demonstrate the high enzymatic activity of these enzymes toward aldehyde odorants. The structure‒function relationship set based on the enzymatic parameters characterization of a series of aldehyde odorant compounds was supported by the X-ray structure resolution of human ALDH3A1 in complex with octanal.

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confidence: 98%

Characterization of human oxidoreductases involved in aldehyde odorant metabolism

Boichot

Ménétrier

Saliou

et al. 2023

Sci Rep

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“…showed the enzymatically driven oxidation of aldehydes to their corresponding carboxylic acid, and the involvement of aldehyde oxidases in mouse. Since AOX are also found in in the human OE this might give a starting point for further investigations [67] . Previous studies additionally showed that the speed of the enzymatic generation of metabolites is in a similar time scale to odor perception (hundreds of milliseconds range) [38] .…”

Section: Odorant Metabolism In the Nasal And Oral Cavities The Lrt An...mentioning

confidence: 93%

“…Since AOX are also found in in the human OE this might give a starting point for further investigations. [67] Previous studies additionally showed that the speed of the enzymatic generation of metabolites is in a similar time scale to odor perception (hundreds of milliseconds range). [38] Consequently, inhibition of the relevant enzymes present in the nose-referred to as odorant metabolizing enzymes (OME)-and thus inhibition of metabolite formation can be suggested to have an impact on odor perception.…”

Section: Odorant Metabolism In the Nasal And Oral Cavities The Lrt An...mentioning

confidence: 97%

Odorant Metabolism in Humans

et al. 2022

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Odorants are relatively small molecules which are easily taken up and distributed in the human body. Despite their relevance in everyday life, however, only a limited amount of evidence about their metabolism, pathways, and bioactivities in the human body exists. With this Review, we aim to encourage future interdisciplinary research on the function and mechanisms of the biotransformation of odorants, involving different disciplines such as nutrition, medicine, biochemistry, chemistry, and sensory sciences. Starting with a general overview of the different ways of odorant uptake and enzymes involved in the metabolism of odorants, a more precise description of biotransformation processes and their function in the oral cavity, the nose, the lower respiratory tract (LRT), and the gastrointestinal tract (GIT) is given together with an overview of the different routes of odorant excretion. Finally, perspectives for future research are discussed.

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“…Speziell bei Personen mit einer idiopathischen Dysfunktion war der Metabolismus von Hexanal deutlich reduziert. [66] In Mäusen beschreiben Takaoka et al (2022) [67] auch eine Oxidation von Aldehyden zu ihren entsprechenden Carbonsäuren. Aldehydoxidasen wurden des Weiteren im menschlichen Riechepithel nachgewiesen, weshalb dies einen vielversprechenden Ansatzpunkt für weitere Forschung darstellt.…”

Section: Geruchsstoffmetabolismus In Nase Mund Atemtrakt Und Git 41 G...unclassified

Geruchsstoffmetabolismus im Menschen

et al. 2022

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Geruchsstoffe sind verhältnismäßig kleine Moleküle, welche vom menschlichen Körper leicht aufgenommen werden können. Trotz ihrer großen Relevanz im Alltag ist vergleichsweise wenig über ihren Metabolismus, ihre Verteilungswege und ihre Bioaktivität im menschlichen Körper bekannt. Mit diesem Aufsatz möchten wir zu interdisziplinärer Forschung an den Schnittstellen zwischen Ernährungswissenschaften, Medizin, Biochemie, Chemie und Wahrnehmungswissenschaften beitragen, um das Wissen über die Mechanismen und Funktion der Biotransformation von Geruchsstoffen zu erweitern. Innerhalb des Artikels wird zunächst ein allgemeiner Überblick über die Aufnahme von Geruchsstoffen und die bei der Verstoffwechselung beteiligten Enzyme gegeben. Anschließend wird speziell auf die Biotransformation im Mund-und Nasenraum sowie in der Lunge und im Gastrointestinaltrakt eingegangen, und die verschiedenen Ausscheidungswege werden aufgezeigt. Schließlich wird ein Ausblick auf die zukünftige Forschung gegeben und diskutiert.

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Involvement of aldehyde oxidase in the metabolism of aromatic and aliphatic aldehyde-odorants in the mouse olfactory epithelium

Cited by 3 publications

References 37 publications

Characterization of human oxidoreductases involved in aldehyde odorant metabolism

Characterization of human oxidoreductases involved in aldehyde odorant metabolism

Odorant Metabolism in Humans

Geruchsstoffmetabolismus im Menschen

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