Involvement of adenosine 5′-triphosphate in ultrasound-induced fracture repair

Hayton, Michael J.; Dillon, J.P.; Glynn, Danielle J.; Curran, Judith M.; Gallagher, James A.; Buckley, Katherine A.

doi:10.1016/j.ultrasmedbio.2005.04.017

Cited by 46 publications

(28 citation statements)

References 49 publications

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“…The cells were subjected to LIPUS with modifications, as reported previously [19,20]. Briefly, a sterilized transducer (Asahi Irika Co, Ltd, Saitama, Japan) generating 1.5-MHz LIPUS in a pulsed-wave mode at 30 mW/cm 2 was placed horizontally into each culture well so that it was barely touching the surface of the medium.…”

Section: Methodsmentioning

confidence: 99%

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Suzuki¹,

Takayama²,

Suzuki³

et al. 2009

ABBS

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There were few studies investigating the effects of the mechanical stimulation provided by daily low-intensity pulsed ultrasound (LIPUS) treatment. LIPUS is known to accelerate bone mineralization and regeneration; however, the precise cellular mechanism is unclear. Our purpose was to determine how daily LIPUS treatment affected cell viability, alkaline phosphatase activity, osteogenesis-related gene expression, and mineralized nodule formation in osteoblasts. The typical osteoblastic cell line ROS 17/2.8 cells were cultured in the absence or presence of LIPUS stimulation. Daily LIPUS treatments (1.5 MHz; 20 min) were administered at an intensity of 30 mW/cm 2 for 14 days.Expression of osteogenesis-related genes was examined at mRNA levels using real-time polymerase chain reaction and at protein levels using western blotting analysis. LIPUS stimulation did not affect the rate of cell viability. Alkaline phosphatase activity was increased after 10 days of culture with daily LIPUS stimulation. LIPUS significantly increased the expression of mRNAs encoding Runx2, Msx2, Dlx5, osterix, bone sialoprotein, and bone morphogenetic protein-2, whereas it significantly reduced the expression of mRNA encoding the transcription factor AJ18. Mineralized nodule formation was markedly increased on Day 14 of LIPUS stimulation. LIPUS stimulation directly affected osteogenic cells, leading to mineralized nodule formation. LIPUS is likely to have a fundamental influence on key functional activities of osteoblasts in alveolar bone.

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Section: Methodsmentioning

confidence: 99%

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Suzuki¹,

Takayama²,

Suzuki³

et al. 2009

ABBS

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“…It has been shown recently that osteoblasts respond to ultrasound stimulation by increasing ATP release, which appears to mediate stimulation of osteoblast gene expression and cell proliferation (Hayton et al, 2005).…”

Section: Musculoskeletal Diseasesmentioning

confidence: 99%

Pathophysiology and Therapeutic Potential of Purinergic Signaling

2006

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“…The amount of ATP released from osteoblasts depends on their differentiation state, with mature, bone-forming cells releasing up to seven-fold more than immature, proliferating cells (Orriss et al, 2009). Several studies have demonstrated enhanced ATP release in response to different external stimuli including hypoxia (Orriss et al, 2009), mechanical stress (Hecht et al, 2013), fluid flow (Genetos et al, 2005;Romanello et al, 2005), vitamin D (Biswas et al, 2009) and ultrasound (Alvarenga et al, 2010;Hayton et al, 2005). A recent investigation examined the effect of different forms of mechanical stimulation on ATP release from human osteoblast-like SaOS-2 cells, including turbulent fluid flow, laminar fluid flow, substrate strain and 3D compressive loading (Rumney et al, 2012).…”

Section: Atp Release From Osteoblastsmentioning

confidence: 99%

The role of purinergic signalling in the musculoskeletal system

Orriss

2015

Autonomic Neuroscience

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Involvement of adenosine 5′-triphosphate in ultrasound-induced fracture repair

Cited by 46 publications

References 49 publications

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Pathophysiology and Therapeutic Potential of Purinergic Signaling

The role of purinergic signalling in the musculoskeletal system

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