Involvement of a novel NAP member LbNAP in flower senescence and dehydration stress in cut lily

LbHSP17.9 Participated in the Regulation of Cold Stress in Cut Lily Flowers

“…The qPCR analysis was performed with Lbction as the internal reference gene. The relative expression was calculated using the 2 −∆∆CT method [16].…”

“…The PTRV2 vector was digested by SacI and BamHI double enzymes, recovered and recombined with the above PCR product LbHSP17.9. The recombinant product was transformed into E. coli, with specific steps according to Liu et al [16].…”

“…For VIGS, the transformation solution containing TRV1, TRV2 and the TRV2::LbHSP17.9 plasmid was adjusted to OD600 of 1.1, respectively, and then was mixed in a volume ratio of 1:1 to obtain the TRV1/TRV2 and TRV1/TRV2::LbHSP17.9 infection solution. The stem of a cut lily was immersed into the infection solution, using the vacuum infection method of Liu et al [16]. Samples were collected every 72 h to measure the relative expression of antioxidant enzyme genes, MDA content and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD).…”

“…The coding sequence of LbHSP17.9 was cloned (primers shown in Supplementary Table S1) into the overexpression vector PC2300S, and the overexpression vector OE-LbHSP17.9 was constructed based on the procedure of Liu et al [16].…”

“…The A. thaliana Col-0 was transformed using the floral dip method (Liu et al, 2023) [16]. Positive plants were selected through kanamycin (50 mg•L −1 ) screening (Supplementary Figure S1) and PCR confirmation (Supplementary Figure S2).…”