Invitro Screening of Antifungal Activity of Rhizospheric Bacteria and Possible Role of Chitinase in Antifungal Activity

Devkota, Hari K.; Maharjan, Bijaya Laxmi; Baral, Bikash; Singh, Anjana; Yami, Kayo Devi

doi:10.3126/njst.v12i0.6517

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…to suppress root rot disease caused by F. oxysporum , causal agent of saffron corm rot. Chitinolytic potential of the isolates might be responsible for their antifungal activity (Devkota et al 2011 ). Results indicate that most of the isolates possess plant growth promotion traits and may have important role in biology of saffron by promoting plant growth directly, indirectly or synergistically.…”

Section: Resultsmentioning

confidence: 99%

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

2015

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Saffron (Crocus sativus) is a medicinally important plant. The Kashmir valley (J&K, India) emblematizes one of the major and quality saffron producing areas in the world. Nonetheless, the area has been experiencing a declining trend in the production of saffron during the last decade. Poor disease management is one of the major reasons for declining saffron production in the area. Endophytes are known to offer control against many diseases of host plant. During the present study, culturable bacterial endophytes were isolated from saffron plant, identified and assessed for plant growth promoting activities. Molecular and phylogenetic analysis grouped the fifty-four bacterial isolates into eleven different taxa, viz. Bacillus licheniformis, B. subtilis, B. cereus, B. humi, B. pumilus, Paenibacillus elgii, B. safensis, Brevibacillus sp., Pseudomonas putida, Staphylococcus hominis and Enterobacter cloacae. The results were also supported with the identification based on BIOLOG system. B. licheniformis was the dominant endophyte in both leaves and corms of saffron. 81 % isolates showed lipase activity, 57 % cellulase, 48 % protease, 38 % amylase, 33 % chitinase and 29 % showed pectinase activity. 24 % of the isolates were phosphate solublizers, 86 % showed siderophore production and 80 % phytohormone production potential. The present repository of well characterized bacterial endophytes of saffron, have plant growth promoting potential which can be explored further for their respective roles in the biology of the saffron plant.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1435-3) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

2015

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“…Chitinase producing bacteria were selected based on the formation of halo zone of clearance around the colony. For the visualization of the chitinolytic activity, the agar plates were flooded with 0.5% congo red solution for 30 min and destained with 1 M NaCl for 20 min .…”

Section: Methodsmentioning

confidence: 99%

Bioprospecting around Arctic islands: Marine bacteria as rich source of biocatalysts

Santi

Altermark

Pascale

et al. 2015

J. Basic Microbiol.

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We have investigated the biotechnological potential of Arctic marine bacteria for their ability to produce a broad spectrum of cold-active enzymes. Marine bacteria exhibiting these features are of great interest for both fundamental research and industrial applications. Macrobiota, water and sediment samples have been collected during 2010 and 2011 expeditions around the Lofoten and Svalbard islands. Bacteria were isolated from this material and identified through 16S rRNA gene sequence analysis for the purpose of establishing a culture collection of marine Arctic bacteria. Herein, we present the functional screening for different extracellular enzymatic activities from 100 diversely chosen microbial isolates incubated at 4 and 20 °C. The production of esterase/lipase, DNase, and protease activities were revealed in 67, 53, and 56% of the strains, respectively, while 41, 23, 9, and 7% of the strains possessed amylase, chitinase, cellulase, and xylanase activities, respectively. Our findings show that phylogenetically diverse bacteria, including many new species, could be cultured from the marine arctic environment. The Arctic polar environment is still an untapped reservoir of biodiversity for bioprospecting.

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Phytochemical And Antimicrobial Characterization Of Rhododendron Anthopogon From High Nepalese Himalaya

Baral

Vaidya

Maharjan

et al. 2015

Botanica Lithuanica

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The biological and chemical properties of the medicinally important high altitudinal plant Rhododendron anthopogon D. Don were assayed. Extracts at a concentration of 100 mg-ml-1 from leaves and flowers were collectively obtained by employing hot extraction method with different solvents. The antibacterial and antifungal properties were assayed against different clinical bacteria (two Gram+ and six Gram-) and six phytopathogenic fungi. Klebsiella pneumoniae and Salmonella typhimurium were the most inhibited bacteria, while Fusarium eridiforme, a fungus, was highly inhibited. However, all the tested bacteria were resistant to n-hexane and chloroform extracts. Of all the extracts obtained, the ethyl acetate fraction, followed by the hot methanolic extract, was effective against all the pathogenic strains tested. The MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values of ethyl acetate and aqueous extracts on bacteria ranged from 1.562 mg-ml” 1 to 25.0 mg-ml” 1, whereas MIC and MFC (minimum fungicidal concentration) values of n-hexane and ethyl acetate fractions on fungi varied from 1.562 mg-ml-1 to 12.50 mg-ml-1, which are slightly higher than expected. The extracts showed no activity against Exserohilum turticum and Stenophylum sp. There was a highly significant difference in the zone of inhibition between different extract fractions and bacterial or fungal strains. A preliminary qualitative phyto-chemical assay revealed the presence of polyphenols, reducing compounds, quinones, sterol, triterpenes and fatty acids in leaves and flowers of the test plant. The observed antimicrobial effects are believed to be due to the presence of these compounds. The broad spectrum of activity of the extracts would apparently explain the widespread use of this plant for controlling human pathogenic bacteria and phytopathogenic fungi that widely destroy crops in Nepal. This research reveals R. anthopogon to be a highly promising source of potent antimicrobial drugs that could be used to design therapeutic drugs in the pharmaceutical industry.

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Invitro Screening of Antifungal Activity of Rhizospheric Bacteria and Possible Role of Chitinase in Antifungal Activity

Cited by 3 publications

References 13 publications

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

Diversity of culturable bacterial endophytes of saffron in Kashmir, India

Bioprospecting around Arctic islands: Marine bacteria as rich source of biocatalysts

Phytochemical And Antimicrobial Characterization Of Rhododendron Anthopogon From High Nepalese Himalaya

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