How does culture influence vaccination acceptance? This is an important question facing managers, policymakers, and global health organizations. Even with effective vaccines for highly contagious diseases, humankind remains at risk from vaccine hesitancy. We conduct a largescale multilevel analysis of more than 400,000 survey respondents, finding that COVID-19 vaccination intentions are higher among people from countries higher in cultural collectivism (Study 1). Follow-up studies indicate that vaccination acceptance is higher among people that endorse collectivistic values because they feel more empathy for those afflicted by the disease (Studies 2a, 2b, 3), especially when victims of the disease have similar characteristics (e.g., political affiliation, lifestyle, personality) as themselves (Study 3). To encourage vaccination acceptance, we suggest promoting collectivistic values and empathic concern, as well as homophily through the portrayal of victims with characteristics like those hesitant to accept vaccination.