“…Human elderly microglia were isolated and maintained in culture as previously described (Lue et al, 1996(Lue et al, , 2001Walker et al, 2005), using methods similar to those originally developed by Kim (1985). The cultured microglia expressed antigenic, morphologic, and other criteria of microglia, and were approximately 98% pure, confirming previous studies (Lue et al, 1996(Lue et al, , 2001Walker et al, 2005).…”