Investigations on the Underlying Principles of Test Methods for Formaldehyde-release from Plywoods and Furnitures

KOJIMA, SHIGEO; Kaniwa, Masa-aki; Nakamura, Akitada

doi:10.1248/jhs1956.28.4_205

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“…Chromatography of the metal-free or apo forms at low pH ( Figure 2) showed that the four subfractions of Figure 1 were resolved into a total of seven individual isoforms designated MTla, MT-2d, MT-2e, MT-2b, MT-2b', MT-2c and MT-2a. The nomenclature division of the mammalian MTs into the major subgroups MT-I and MT-II which was initially based on charge differences now refers to the absence or presence of an acidic amino acid residue in position 10 or 11 of mammalian sequences, respectively [19]. While fractions B (MT-2e) and C (MT-2b) were homogeneous after the initial preparative chromatography The isoforms shown in Figure 2 (A and B) were purified by repeated chromatography on a semipreparative Aquapore RP-300 column (Brownlee, 7 x 250 mm) at a flow rate of 1.5 ml/min using the conditions given in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

Primary structures of seven metallothioneins from rabbit tissue

Hunziker

Kaur

Wan

et al. 1995

Biochemical Journal

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Metallothionein from tissues of rabbits exposed to cadmium chloride was separated into seven distinct isoforms by reverse-phase liquid chromatography and their complete amino acid sequences were determined. Five of the seven isometallothioneins showed structural features so far not identified in other mammalian metallothioneins. Thus, two isoproteins contain a polypeptide with a chain length of 62 rather than 61 amino acid residues. Two isoforms are characterized by an additional positive charge and one by the presence of an isopeptide bond between aspartic acid and serine in the N-terminal half of the protein. The isoproteins characterized were identified from different sources: rabbit liver and kidney and a rabbit kidney cell-line (RK-13). In all three, the structural characteristics of the individual isoforms are retained, indicating that in the different tissues the same mechanisms control the synthesis and the stability of the different cadmium-induced isoMTs.

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Section: Resultsmentioning

confidence: 99%