Investigations on the compatibility of chemically oxidized silicon (SiO )-surfaces for applications towards chip-based polymerase chain reaction

Towards a portable microchip system with integrated thermal control and polymer waveguides for real‐time PCR

“…Possible surface-induced inhibition is always a critical issue in microfabricated PCR devices [42,[48][49][50][51]. To avoid any inhibition from the SU-8 surface, 1 mg/mL nonacetylated BSA was added to the PCR mix.…”

Surface Modification of Microchip Input Reservoirs for Pressure-induced Sample Injection into Microreactors

“…10 The contact angles of water at the Si surfaces were found to be 45 -50˚, 75 -80˚ and 85 -90˚ for untreated chips, after CDMOS and DCDMS modifications, respectively. Since the DCDMS surface resulted in larger contact angles, and also showed enhanced stability (boiling in water for 2 h without any contact angle deterioration), DCDMS was chosen as an optimal surface modifier.…”

“…10 The outer chip surface and internal microfluidic structures were pretreated by ∼75% perchloric acid for 2 -3 h at 130 -140˚C. Chips were successively washed with water, acetone, dried at 80˚C for 5 min and then rinsed with dry toluene.…”

Instrumentation of a PLC-Regulated Temperature Cycler with a PID Control Unit and Its Use for Miniaturized PCR Systems with Reduced Volumes of Aqueous Sample Droplets Isolated in Oil Phase in a Microwell

“…Among the DNA assay chips, a number of miniaturized instruments have been developed for polymerase chain reactions (PCR) because the PCR amplification is widely used as a molecular biological tool to replicate millions of copies of target DNA fragments by cycling through two or three temperature steps (denaturation, annealing and extension). [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] The miniaturization of PCR devices can take advantage of reduced consumption of biological sample necessary for PCR and of increased portability. In addition, the decreased cost of fabrication with a choice of polymer materials as a substrate for PCR reaction vessel allows one-time use of the chips, leading to elimination of false positive data resulting from carryover crosscontamination.…”

“…In the batch format, a sample is injected into a confined space in the chip and then repeatedly heated and cooled according to the reaction procedure. [1][2][3][4][5][6][7][8][9][10][11][12][13] In the continuous-flow format, DNA amplification can be achieved by shuttling a PCR cocktail in a microchannel repetitively through different isothermal zones. [14][15][16][17][18][19] The batch format chip is more suitable for miniaturization and high throughput operation; however, special care must be taken in the thermal management of a stationary PCR mixture to acquire quick transitions having low overshoots, together with good rejection of the disturbance.…”