Investigations on the amino acid secretion and absorption in the stomach of the growing pig

Żebrowska, T.; Simon, O.; Münchmeyer, R; Bergner, H.; Zebrowska, H

doi:10.1080/17450398209425652

Cited by 13 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the pigs being fed different proteins and different ways of labelling with 15 N being used, the experimental data obtained supports the results of our previous study Zebrowska et al, 1992) and are in agreement with other experiments; Zebrowska et al (1982) and Souffrant et al (1986) indicating its validity for growing pigs. In the present experiment, N retention, related to intake, was 10% lower in comparison to the soyabean meal diet of the previous experiment (Zebrowska et al, 1992) due to lower apparent digestibility and higher urinary N excretion.…”

Section: Discussionsupporting

confidence: 81%

Intestinal nitrogen flow, total nitrogen and 15N balance in pigs labelled intravenously with 15N-leucine and fed a meat meal diet

Schadereit¹,

Krawielitzki²,

Żebrowska³

et al. 1995

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Three pigs of about 33 kg body weight, each fitted with re-entrant cannulas into the duodenum and ileum and with bladder catheters were used for the study. One of the pigs was infused intravenously with 15 N-leucine for 8 days. Total N passage, 15 N nitrogen passage through the duodenum and ileum, and N balance were determined. The mean amount of total N passing the duodenum exceeded the ingested N by 12%, and that passing the ileum accounted for 27% of N intake. Of the infused 15 N, 67.4% was found in the urine, faeces, digesta and carcass after slaughter. Taking the recovered amount of 15 N as 100%, 20.4% was found in urine, 0.6% in faeces, 8.2% in digesta and 70.8% in tissues. It was concluded that 15 N retention from intravenously administrated leucine was much higher than from 15 N in urea given with feed, as estimated previously.

show abstract

Section: Discussionsupporting

confidence: 81%

Intestinal nitrogen flow, total nitrogen and 15N balance in pigs labelled intravenously with 15N-leucine and fed a meat meal diet

Schadereit¹,

Krawielitzki²,

Żebrowska³

et al. 1995

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…This may be due both to the greater endogenous N secretion in the pigs given soya-bean protein and to the lower absorption of exogenous and endogenous N before the terminal ileum. The digestibility of endogenous N is less than the dietary N (Buraczewska et al 1975;Zebrowska et al 1976); while Zebrowska (1973) has shown that amounts of endogenous N of pigs given SBM, particularly in the proximal small intestine, were twice that obtained from pigs given other protein sources.…”

Section: Discussionmentioning

confidence: 99%

“…The values of the present experiment may be explained by the younger age of the pigs and by the frequency of feeding. The pigs in Expt 4 were fed every 2 h while those of Holmes et al (1974) and Zebrowska et al (1975) were fed twice daily. This frequent feeding may stimulate greater digestive secretions as Corring et al (1972) showed that there was an increase in protein secretion by the pig's pancreatic secretions in response to feeding.…”

Section: Discussionmentioning

confidence: 99%

Digestion in the pig between 7 and 35 d of age

Wilson

Leibholz

1981

Br J Nutr

View full text Add to dashboard Cite

1. In two separate experiments, forty-four pigs weaned at 4-5 d of age were fed on diets containing milk or soya-bean protein until slaughtered at 14, 28 or 35 d of age.2. Daily amounts of nitrogen flowing through the stomach and proximal small intestine of pigs were similar with both sources of protein fed.3. Endogenous N flows in the duodenum, jejunum and ileum of pigs given an N-free diet were 22.9,7.4 and 4.6 g N/kg dry matter (DM) intake. Endogenous N in faeces was 0.970 g N/kg DM intake. 7.Of the N in the stomach 1432% could not be precipitated by trichloroacetic acid (TCA; 30 g/l). 8. In the jejunal and ileal contents an average of 46 and 24% of the N was precipitated by TCA in pigs given soya-bean proteins and milk proteins respectively.It is well known that the performance of pigs of 14 d of age or less is lower when they are fed on diets containing soya-bean protein than diets containing milk proteins (e.g. Hays et al. 1959). The over-all apparent digestibility of soya-bean protein is less than that of milk protein, but there have been no studies on the sites of nitrogen absorption and endogenous N secretions in pigs up to 35 d of age, although similar studies have been made with older pigs (e.g. Zebrowska, 1973). Hence, a study of the absorption of N was carried out with young pigs given milk or soya-bean proteins. E X P E R I M E N T A LAnimals and diets Expt 3. Thirty-six pigs (mean weight 2-05 kg, mean age 7 d) were allocated to six diets as a 3 x 2 factorial with three replicates of two pigs per replicate. Three protein sources were compared in isonitrogenous diets: milk, isolated soya-bean protein (ISP) (Promine D;Central Soya Co., Chicago, Ill.) and soya-bean meal (SBM), each with and without methionine supplementation, and lysine supplementation of the ISP and SBM diets. The composition of the diets is given in Wilson & Leibholz (1981 a). After 17 d, one pig per pen was removed and the remaining eighteen pigs were given the experimental diets sprayed with indigestible markers for a further 5 d. The pigs were then slaughtered at 28 d of age as described in Wilson & Leibholz (1981 b).

show abstract

“…The total amount of nitrogenous material entering the gut lumen is influenced by various dietary factors. It has been known for many years, for example, that the gut enzymatic secretions are influenced by both the amount and type of protein ( 9 , 10 ) . Plant non starch polysaccharides and potentially, resistant starch can greatly influence the loss of endogenous protein at the end of the ileum ( 11 , 12 ) .…”

Section: Sites and Sources Of Endogenous Proteinmentioning

confidence: 99%

Gut luminal endogenous protein: Implications for the determination of ileal amino acid digestibility in humans

Moughan

Rutherfurd

2012

Br J Nutr

View full text Add to dashboard Cite

The true ileal digestibility assay provides the most informative measure of digestibility to assess bioavailability of amino acids in foods for humans. To determine 'true' estimates of ileal amino acid digestibility, requires that endogenous amino acids present in digesta at the terminal ileum be quantified. The amounts of endogenous amino acids in ileal digesta can be determined after feeding an animal or human a protein-free diet (traditional approach) or by various methods after giving a protein-containing diet. When the protein-free method has been applied with adult human subjects an overall mean value (three separate studies) for endogenous ileal nitrogen flow of 800 mg N/d has been reported. This value is considerably lower than a comparable value obtained after feeding protein of 1852 mg N/d (mean of four separate studies), and thus endogenous ileal N and amino acids should be measured under conditions of protein alimentation. There is some confusion concerning the terminology used to define digestibility, with the term "true" digestibility having different adopted meanings. Here, true amino acid digestibility is defined as apparent amino acid digestibility corrected for the basal amino acid losses determined after giving either a protein-free or a protein-containing diet. Basal losses should be determined at a defined dry-matter and protein intake. The protein-free diet approach to determining endogenous amino acids is considered unphysiological and basal losses refer to ileal endogenous amino acid flows associated with digesta dry-matter flow, and not including "specific" effects of dietary factors such as non starch polysaccharides and anti nutritional factors. Arguments are advanced that the enzyme hydrolysed protein/ultra filtration method may be suitable for routine application with a cannulated pig model, to obtain physiologically-valid basal estimates of ileal endogenous amino acids to allow calculation of true ileal amino acid digestibility in the pig, and then prediction (via statistical relationships) of true coefficients of amino acid digestibility in humans.

show abstract

Investigations on the amino acid secretion and absorption in the stomach of the growing pig

Cited by 13 publications

References 5 publications

Intestinal nitrogen flow, total nitrogen and <sup>15</sup>N balance in pigs labelled intravenously with <sup>15</sup>N-leucine and fed a meat meal diet

Intestinal nitrogen flow, total nitrogen and <sup>15</sup>N balance in pigs labelled intravenously with <sup>15</sup>N-leucine and fed a meat meal diet

Digestion in the pig between 7 and 35 d of age

Gut luminal endogenous protein: Implications for the determination of ileal amino acid digestibility in humans

Contact Info

Product

Resources

About