Investigations on Putative Zebra Chip Tolerant Potato Selections

Lévy, Julien; Scheuring, Douglas C.; Koym, Jeffrey W.; Henne, D. C.; Tamborindeguy, Cécilia; Pierson, Elizabeth A.; Miller, J. Creighton

doi:10.1007/s12230-015-9452-x

Cited by 21 publications

(9 citation statements)

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“…verrucosum [ 14 , 15 , 16 ] and to unique breeding clones derived from Solanum etuberosum and S . berthaultii [ 17 , 10 ], as well as various breeding clones response to Lso [ 18 , 11 ] have previously been reported.…”

Section: Introductionmentioning

confidence: 99%

Characterization of host plant resistance to zebra chip disease from species-derived potato genotypes and the identification of new sources of zebra chip resistance

et al. 2017

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‘Candidatus Liberibacter solanacearum’ (Lso), an uncultivable phloem-limited phytopathogenic bacteria, is known to be associated with Zebra Chip disease (ZC), which represents a major threat to potato production in the US and elsewhere. This pathogen is transmitted by the phloem-feeding potato psyllid, Bactericera cockerelli Sulc (Hem. Triozidae). Currently, there are no reports of resistance to ZC in cultivated potatoes. This greenhouse study was conducted to evaluate Lso transmission success and the susceptibility of 11 potato breeding clones, representing diverge genetic background, to ZC, in relation to a susceptible commercial cultivar, Russet Burbank. Individual plants were exposed to two Lso-positive potato psyllids for 48 hours. The percentage of successful Lso transmission varied across the evaluated genotypes ranging between 7 and 57%. Freshly-cut and fried tubers showed significant variation in ZC symptom severity among the breeding clones, with several genotypes expressing relative tolerance when compared to Russet Burbank. None of the evaluated clones showed statistically lower Lso titers than Russet Burbank with the exception of one genotype in the second year of the study. However, the presence of a non-significant relationship between average symptom severity and Lso titer indicated variations in phenotypic responses (i.e. tolerance) to Lso existed among evaluated breeding lines. Breeding clones A07781-3LB, A07781-4LB and A07781-10LB had relatively lower Lso titer (low susceptibility) and tuber symptom expression (high tolerance) among the tested genotypes. As these three clones represent full siblings, the observed effects could be indicative of the presence of a genetic basis for resistance/tolerance to ZC. Findings provide a better understanding of resistance/tolerance to ZC, and contribute to continued efforts in breeding for resistance to this disease.

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Section: Introductionmentioning

confidence: 99%

Characterization of host plant resistance to zebra chip disease from species-derived potato genotypes and the identification of new sources of zebra chip resistance

et al. 2017

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“…Currently, potato psyllid control relies on insecticide applications but even with conventional insecticides, this pest is difficult to manage [25]. Furthermore, commercially acceptable genetic resistance against the potato psyllid or Lso has not yet been identified in potato or other solanaceous crops [26]. Therefore, it is crucial to explore alternative strategies to control potato psyllids.…”

Section: Introductionmentioning

confidence: 99%

Concanavalin A Toxicity Towards Potato Psyllid and Apoptosis Induction in Midgut Cells

Tang

Ibáñez

Tamborindeguy

2020

Insects

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Concanavalin A (ConA), a legume lectin, has been drawing increasing attention in recent years concerning its toxicity against insects and its potential application in pest management. In an attempt to evaluate the effect of ConA on potato psyllid (Bactericera cockerelli), an economically important pest of solanaceous crops, the effect of ConA on potato psyllid survival, psyllid gut nuclear morphology, and expression of psyllid caspase genes were evaluated. Our results determined that artificial diet-feeding assays using ConA had deleterious effects on potato psyllids, resulting in significant psyllid mortality following ingestion. We also found that an apoptotic response was induced by ConA in psyllid midgut cells, which was demonstrated by the DNA fragmentation and abnormal nuclear architecture in the midgut cells. Following ConA ingestion, there was also upregulation of caspase genes in the psyllid midguts. Therefore, a key mechanism behind ConA toxicity towards potato psyllid probably involves the induction of apoptosis in midgut cells. This study could provide a better understanding of the mechanisms underlying ConA toxicity in insects and be a stepping stone towards the development of new psyllid control strategies based on plant lectins.

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“…Multiple reports indicate neonicotinoid resistance is increasing in certain B. cockerelli populations (Bass et al, 2015;Nauen & Denholm, 2005;Prager et al, 2013). Since vector-borne disease systems are faced with the rapid evolution of pesticide resistance, major efforts have been made to develop novel solutions based on selectively breeding plants for improved host-plant resistance or genetically manipulating plants and insects for the purpose of disrupting disease transmission (Avila et al, 2019;Barzman et al, 2015;Lévy et al, 2015;Levy & Tamborindeguy, 2014;San Miguel & Scott, 2016;Whalon et al, 2008). For example, disease transmission can be disrupted by manipulating the host or vector's genes associated with key molecular pathways that facilitate the movement of pathogens from host to vector and vice versa (Almeida et al, 2014;Kumar et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Lasting consequences of psyllid (Bactericera cockerelli L.) infestation on tomato plant gene expression

Harrison

Mendoza-Herrera

Lévy

et al. 2020

Preprint

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Background. The tomato psyllid, Bactericera cockerelli Šulc (Hemiptera: Triozidae), is a pest of solanaceous crops such as tomato (Solanum lycopersicum L.) in the U.S. and vectors the disease-causing pathogen ‘Candidatus Liberibacter solanacearum’. Currently, the only effective strategies for controlling the diseases associated with this pathogen involve regular pesticide applications to manage psyllid population density. However, such practices are unsustainable and will eventually lead to widespread pesticide resistance in psyllids. Therefore, new control strategies must be developed to increase host-plant resistance to insect vectors. For example, expression of constitutive and inducible plant defenses can be improved through selection. Currently, it is still unknown whether psyllid infestation has any lasting consequences on tomato plant defense or tomato plant gene expression in general. Results. In order to characterize the genes putatively involved in tomato defense against psyllid infestation, RNA was extracted from psyllid-infested and uninfested tomato leaves (Moneymaker) three weeks post-infestation. Transcriptome analysis identified 362 differentially expressed genes. These differentially expressed genes were primarily associated with defense responses to abiotic/biotic stress, transcription/translation, cellular signaling/transport, and photosynthesis. These gene expression changes suggested that tomato plants underwent a reduction in plant growth/health in exchange for improved defense against stress that was observable three weeks after psyllid infestation. Consistent with these observations, tomato plant growth experiments determined that the plants were shorter three weeks after psyllid infestation. Furthermore, psyllid nymphs had lower survival rates on tomato plants that had been previously psyllid infested. Conclusion. These results suggested that psyllid infestation has lasting consequences for tomato gene expression, defense, and growth.

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Investigations on Putative Zebra Chip Tolerant Potato Selections

Cited by 21 publications

References 20 publications

Characterization of host plant resistance to zebra chip disease from species-derived potato genotypes and the identification of new sources of zebra chip resistance

Characterization of host plant resistance to zebra chip disease from species-derived potato genotypes and the identification of new sources of zebra chip resistance

Concanavalin A Toxicity Towards Potato Psyllid and Apoptosis Induction in Midgut Cells

Lasting consequences of psyllid (Bactericera cockerelli L.) infestation on tomato plant gene expression

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