Investigations on Hydrolytic Activities from Stachybotrys microspora and Their Use as an Alternative in Yeast DNA Extraction

Abdeljalil, Salma; Hmad, Ines Ben; Saibi, Walid; Amouri, Bahia; Maalej, Wiem; Kaaniche, Marwa; Koubaa, Aida; Gargouri, Ali

doi:10.1007/s12010-013-0608-1

Cited by 14 publications

(6 citation statements)

References 32 publications

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“…The purified enzyme is a monomeric protein of 78 kDa molecular weight and exhibits optimal activity at pH 6.0 and at 50°C. Recently, the thermostable family 3 β-glucosidase from S. microspora was analysed for its molecular characterization, and mRNA expression (Abdeljalil et al 2014). …”

Section: Stachybotrys In Biocontrol During Mycoparasitism Of Hyphae Amentioning

confidence: 99%

Overview of Stachybotrys (Memnoniella) and current species status

et al. 2015

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Stachybotrys (asexual Hypocreales) has a worldwide distribution. This genus inhabits substrates rich in cellulose and is closely related to Memnoniella. Classification of species has previously been based on morphology, with conidial characters being considered as important. This study reevaluates Stachybotrys and Memnoniella, which is shown to include at least seven species-groups; while Memnoniella is a synonym of Stachybotrys. The sexual genera Ornatispora and Melanopsamma are also synonyms of Stachybotrys. With the exception of Stachybotrys subsimplex, species formed a wellsupported monophyletic group in LSU data analysis belonging to Stachybotriaceae. Seventy-four accepted Stachybotrys species are discussed, while eight species are considered to belong to other genera or are doubtful in this paper and a key to these species provided. Twelve new combinations and 1 nomina nova is proposed. The status of Stachybotrys species on health, as human or animal pathogens, in indoor environments, and use as biocontrol agents and compound discovery are also discussed.

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Section: Stachybotrys In Biocontrol During Mycoparasitism Of Hyphae Amentioning

confidence: 99%

Overview of Stachybotrys (Memnoniella) and current species status

et al. 2015

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“…The major enzyme was much highly expressed in the three raw carbon sources while the minor one was slightly more produced on gruel. The wide majority of fungal species produce one or two β-glucosidases, such as T. reesei or Penicillium occitanis while few others secreted several β-glucosidases, such as S. microspora that produced at least three large and two small β-glucosidases [28].…”

Section: Effect Of Carbon Sources On β-Glucosidase Productionmentioning

confidence: 99%

Fast activated charcoal prepurification of Fusarium solani β-glucosidase for an efficient oleuropein bioconversion

Boudabbous

Saibi

Bouallagui

et al. 2016

Preparative Biochemistry & Biotechnology

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Fungal β-glucosidases were extensively studied regarding their various potential biotechnology applications. Here, we report the selection of Fusarium solani strain producing high yield of β-glucosidase activity. The effect of some factors on β-glucosidase production was studied including: Initial pH, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. The optimal enzyme production was obtained with 4 units of pH. The highest β-glucosidase activity was produced on 4% wheat bran (WB) as raw carbon sources, reaching 5 U/mL. A positive correlation between WB particle size and the β-glucosidase production level was settled. The last one was enhanced to 13.60 U/mL in the presence of 0.5% (w/v) of ammonium sulfate. Interestingly, the activated charcoal was used as an inexpensive reagent enabling a rapid and efficient purification prior step that improved the enzyme-specific activity. Eventually, F. solani β-glucosidase acts efficiently during the bioconversion process of oleuropein. Indeed, 82.5% of oleuropein was deglycosylated after 1 hr at 40°C. Altogether, our data showed that the β-glucosidase of F. solani has a potential application to convert oleuropein to ameliorate food quality.

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“…These properties are exceptional in fungal cellulases. In addition to cellulolytic enzymes and in particular several β-glucosidases [ 22 , 27 ], S. microspora secretes other hydrolytic activities such as proteases and chitinases [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Stable and effective eco-enzyme cocktails in powder and liquid form of Stachybotrys microspora used as detergent additives

Ben Hmad,

Gargouri

2024

Heliyon

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Investigations on Hydrolytic Activities from Stachybotrys microspora and Their Use as an Alternative in Yeast DNA Extraction

Cited by 14 publications

References 32 publications

Overview of Stachybotrys (Memnoniella) and current species status

Overview of Stachybotrys (Memnoniella) and current species status

Fast activated charcoal prepurification of Fusarium solani β-glucosidase for an efficient oleuropein bioconversion

Stable and effective eco-enzyme cocktails in powder and liquid form of Stachybotrys microspora used as detergent additives

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