Investigations on antipyrine metabolism: II—Analysis of conjugated metabolites of antipyrine in rat and human urine by off-line combination of liquid chromatography and field desorption mass spectrometry

Lehmann, Wolf D.; Böttcher, J.; Bässmann, H.; Schüppel, R.; Schiebel, Hans‐Martin

doi:10.1002/bms.1200091105

Cited by 11 publications

(1 citation statement)

References 33 publications

(3 reference statements)

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“…by field desorption (Boettcher et al 1982a;Lehmann et al, 1982), or by electronic impact (Hignite et al, 1978). Our results gave m/z fragmentations similar to those obtained by the abovementioned authors.…”

Section: Sm (Fab Thiogycerol): 373 (Mhmentioning

confidence: 96%

Isolation and purification of three glucuronides of antipyrine. Proposal for an original analytical method for quantitation of sulpho‐ and glucuroconjugated metabolites

Palette¹,

Cordonnier²,

Tillequin³

et al. 1994

Biomedical Chromatography

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The determination of the concentrations of antipyrine metabolites in biological fluids is hampered by the difficulty in obtaining pure conjugated compounds to be used as standards. Most authors have proposed determination of total forms by high performance liquid chromatography (HPLC) after deconjugation of these metabolites using chemical or enzymatic hydrolysis. Up to now there is no satisfactory hydrolysis method for the study of all antipyrine metabolites. The situation is further complicated by the fact that the deconjugated metabolites are highly unstable whichever technique is being used. Because of the lack of stability of all these molecules it has been necessary to isolate the glucuroconjugated compounds from urine. We describe a method which allows us to obtain highly purified glucuroconjugated metabolites of antipyrine. Sulphoconjugated compounds have been synthesized previously. We are thus able to propose a chromatographic procedure which allows us to determine simultaneously all stable phase I and phase II metabolites of antipyrine in biological fluids without any step of extraction. This analytical technique allows us to study the activity of the different isoenzymes implicated in the metabolism of antipyrine.

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Section: Sm (Fab Thiogycerol): 373 (Mhmentioning

confidence: 96%

Isolation and purification of three glucuronides of antipyrine. Proposal for an original analytical method for quantitation of sulpho‐ and glucuroconjugated metabolites

Palette¹,

Cordonnier²,

Tillequin³

et al. 1994

Biomedical Chromatography

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Positive and negative liquid secondary ion mass spectrometry of chloro- and nitrophenylglucuronides

Wickramanayake

Deinzer

Burlingame

1985

Biol. Mass Spectrom.

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Mass spectra of a series of chloro- and nitrophenylglucuronides by liquid secondary ion (LSI) mass spectrometry were obtained. In the positive ion mode class characteristic fragmentations and adduct ions are observed only in the presence of alkali salt additives. No additives were necessary in the negative ion mode to see abundant class characteristic [M-H]- and aglycone fragment ions. Cluster ion formation was found to be prominent but only in the negative ion mode.

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Investigations on antipyrine metabolism V. Differentiation of two isomeric hydroxyantipyrine glucuronides by field desorption and fast atom bombardment mass spectrometry

Lehmann

Schiebel

Böttcher³

et al. 1985

Biol. Mass Spectrom.

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The glucuronide conjugates of the two isomeric antipyrine phase I metabolites of antipyrine in man, 4-hydroxyantipyrine and 3-hydroxymethylantipyrine have been analysed by field desorption and fast atom bombardment mass spectrometry. These isomers could be clearly distinguished on the basis of their fragmentation behaviour which was found to correlate with that observed under pyrolysis electron impact conditions.

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Investigations on antipyrine metabolism: II—Analysis of conjugated metabolites of antipyrine in rat and human urine by off-line combination of liquid chromatography and field desorption mass spectrometry

Cited by 11 publications

References 33 publications

Isolation and purification of three glucuronides of antipyrine. Proposal for an original analytical method for quantitation of sulpho‐ and glucuroconjugated metabolites

Isolation and purification of three glucuronides of antipyrine. Proposal for an original analytical method for quantitation of sulpho‐ and glucuroconjugated metabolites

Positive and negative liquid secondary ion mass spectrometry of chloro- and nitrophenylglucuronides

Investigations on antipyrine metabolism V. Differentiation of two isomeric hydroxyantipyrine glucuronides by field desorption and fast atom bombardment mass spectrometry

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