Investigations into the production and function of leukotrienes during histotropic development of Oesophagostomum dentatum

Daugschies, Arwid

doi:10.1007/s004360050138

Cited by 23 publications

(14 citation statements)

References 25 publications

(21 reference statements)

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“…This is in contrast to studies on the role of eicosanoids in the migration and growth of O. dentatum, biological functions which are both reduced by inhibition of prostaglandin production (Daugschies 1995(Daugschies , 1996Daugschies and Ruttkowski 1998).…”

Section: Biochemical Parameters Of Exsheathmentcontrasting

confidence: 65%

“…The choice of test substances that were evaluated was based on earlier observations that eicosanoids are produced by O. dentatum in vitro and that their inhibition leads to retarded growth and viability (Daugschies 1995(Daugschies , 1996. We therefore used inhibitors of prostaglandin Hsynthase (cyclooxygenase; COX) and lipoxygenase (LOX) as described in Table 1.…”

Section: Parasite Materialsmentioning

confidence: 99%

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Ecdysis of Oesophagostomum: possible involvement of eicosanoids and development of a bioassay

2005

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Bioassays were developed and applied to test the role of eicosanoids and pH changes in ecdysis of Oesophagostomum dentatum. Exsheathment (80-100%) was achieved by subjecting third-stage larvae (L3) either to chlorine (hypochlorite assay) for 5 min or by incubating them in HCl followed by addition of NaHCO3 (pH-change assay) with subsequent cultivation at 38.5 degrees C/10% CO2 for 1 week. Addition of the lipoxygenase (LOX) inhibitor diethylcarbamacine (DEC) to the larvae resulted in a reduction of the exsheathment rates which could be restored by the addition of leukotrienes (LT)B4, LTC4, LTD4 and LTE4. Addition of the cyclooxygenase (COX) inhibitor acetylsalicylic acid (ASA) also resulted in decreased exsheathment rates both in the hypochlorite and in the pH-change assays in a dose-dependent manner. However, the primary COX products (prostaglandins) were not able to reverse this effect, in contrast to LTC4. It was concluded that: (1) both tests are suitable for bioassaying the effect of substances on exsheathment, and (2) eicosanoids involved in the control of exsheathment of L3 of O. dentatum are primarily LT.

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Section: Biochemical Parameters Of Exsheathmentcontrasting

confidence: 65%

Section: Parasite Materialsmentioning

confidence: 99%

Ecdysis of Oesophagostomum: possible involvement of eicosanoids and development of a bioassay

2005

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“…Oesophagostomum dentatum is a nodular parasitic worm that infects the swine intestine. This arachidonic acid-containing parasite produces both prostaglandins (PGE 2 , PGD 2 , 6-keto-PGF 1␣ , PGF 2␣ and TxB 2 ) and leukotrienes (LTB4 and cysteinyl leukotrienes) endogenously (26,58). Inhibitors of both cyclooxygenase and lipoxygenase prevented migration and growth of the worm and, more specifically, prevented development to stage 4 larvae (26)(27)(28).…”

Section: Helminthsmentioning

confidence: 99%

Production of Eicosanoids and Other Oxylipins by Pathogenic Eukaryotic Microbes

2003

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Oxylipins are oxygenated metabolites of fatty acids. Eicosanoids are a subset of oxylipins and include the prostaglandins and leukotrienes, which are potent regulators of host immune responses. Host cells are one source of eicosanoids and oxylipins during infection; however, another potential source of eicosanoids is the pathogen itself. A broad range of pathogenic fungi, protozoa, and helminths produce eicosanoids and other oxylipins by novel synthesis pathways. Why do these organisms produce oxylipins? Accumulating data suggest that phase change and differentiation in these organisms are controlled by oxylipins, including prostaglandins and lipoxygenase products. The precise role of pathogen-derived eicosanoids in pathogenesis remains to be determined, but the potential link between pathogen eicosanoids and the development of TH2 responses in the host is intriguing. Mammalian prostaglandins and leukotrienes have been studied extensively, and these molecules can modulate Th1 versus Th2 immune responses, chemokine production, phagocytosis, lymphocyte proliferation, and leukocyte chemotaxis. Thus, eicosanoids and oxylipins (host or microbe) may be mediators of a direct host-pathogen “cross-talk” that promotes chronic infection and hypersensitivity disease, common features of infection by eukaryotic pathogens

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“…They were amongst the first developmentally regulated molecules described for O. dentatum (Daugschies, 1995; 1996; Daugschies and Ruttkowski, 1998). In mammals, they are known to exert complex control over the development of organ systems, such as the immune and nervous systems, but very little is known about them in nematodes (Daugschies and Joachim, 2000).…”

Section: Nematode-host Interactions and Immunobiologymentioning

confidence: 99%

Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock

Tyagi

Joachim

Ruttkowski

et al. 2015

Biotechnology Advances

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Many infectious diseases caused by eukaryotic pathogens have a devastating, long-term impact on animal health and welfare. Hundreds of millions of animals are affected by parasitic nematodes of the order Stronglida. Unlocking the molecular biology of representatives of this order, and understanding nematode-host interactions, drug resistance and disease using advanced technologies could lead to entirely new ways of controlling the diseases that they cause. Oesphagostomum dentatum (nodule worm; superfamily Strongyloidea) is an economically important strongylid nematode of swine worldwide. The present article reports recent advances made in biology and animal biotechnology through the draft genome and developmental transcriptome of O. dentatum, in order to support biological research of this and related parasitic nematodes as well as the search for new and improved interventions. This first genome of any member of the Strongyloidea is 443 Mb in size and predicted to encode 25,291 protein-coding genes. Here, we review the dynamics of transcription throughout the life cycle of O. dentatum, describe double-stranded RNA interference (RNAi) machinery and infer molecules involved in development and reproduction, and in inducing or modulating immune responses or disease. The secretome predicted for O. dentatum is particularly rich in peptidases linked to interactions with host tissues and/or feeding activity, and a diverse array of molecules likely involved in immune responses. This research progress provides an important resource for future comparative genomic and molecular biological investigations as well as for biotechnological research toward new anthelmintics, vaccines and diagnostic tests.

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Investigations into the production and function of leukotrienes during histotropic development of Oesophagostomum dentatum

Cited by 23 publications

References 25 publications

Ecdysis of Oesophagostomum: possible involvement of eicosanoids and development of a bioassay

Ecdysis of Oesophagostomum: possible involvement of eicosanoids and development of a bioassay

Production of Eicosanoids and Other Oxylipins by Pathogenic Eukaryotic Microbes

Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock

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