Investigations into an outbreak of corvid respiratory disease associated with<i>Pasteurella multocida</i>

Strugnell, Ben; Dagleish, Mark P.; Bayne, C.W.; Brown, Marg; Ainsworth, H. L.; Nicholas, R. A. J.; Wood, Ann R.; Hodgson, John

doi:10.1080/03079457.2011.571659

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…In this study, 23 strains of P. multocida isolated from poultry in Chongqing and Sichuan, China were tested for capsular antigens and antimicrobial susceptibility. The results showed that the capsular types of isolates were predominantly type A which is similar to that it was usual reported for P. multocida in China and other countries [34,35] . But we found one isolate of P. multocida was type F which is similar to the findings that reported previously [33] .…”

Section: Discussionsupporting

confidence: 77%

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

Li¹,

Cheng²,

Lan³

et al. 2017

Kafkas Univ Vet Fak Derg

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Pasteurella multocida (P. multocida) causes fowl cholera in birds and the pathogenesis and virulence factors involved are still poorly understood. TonB protein is a periplasmic protein prevalent in a large number of Gram-negative bacteria, as in Pasteurella, which is believed to be responsible for the import of ferric iron complexes across the outer membrane and plays a role as an important virulence factor to help bacteria to obtain nutrients. In this study, a total of 23 isolates of P. multocida were obtained from 172 cases of clinical respiratory disease in duck and geese, giving an isolation rate of 13.4%. P. multocida Serogroup A was isolated from 22 cases (95.7%), whereas serogroup F was from 1 case (4.3%). All isolates were analyzed for their susceptibility to 15 antibiotics and the presence of 18 genes for virulence factors. The susceptibility profiles suggested that Tetracycline, Cephalosporin, Enrofloxacin, and Aminoglycosides were the drugs most likely to be active against P. multocida. However, 78.3% and 52.2% of poultry strains were resistant to Penicillin and Sulfisoxazole, respectively. PCR results showed that ptfA, fimA, tonB, fur, hgbA, hgbB, sodA, sodC, pmHAS, nanH, nanB, plpB and ompH genes occur in most poultry strains of P. multocida. The virulence genes such as toxA, tadD, hsf-1, pfhA and ompA were each present in <73.9% of strains. The tonB gene was detected in all 23 clinical strains of P. multocida which were recovered from different hosts and harboring different serotypes. Phylogenetic analysis indicated that these tonB genes of P. multocida from different hosts and harboring different serotypes have close genetic relationship with a high similarity. Multiple sequence alignment demonstrated that TonB protein of Gram-negative bacteria exists in multiple conserved sites, such as -xSSGx-, -YP-, -LD-and xA[A/V]Lx motif. These findings provide clinical data into the epidemiological and molecular characteristics of avian P. multocida isolates and provide a reference for the researches of drug target in P. multocida. Keywords: Antimicrobial susceptibility, Capsular antigens, Pasteurella multocida, tonB gene, Virulence gene, Cloning, Phylogenetic analysis Çin'de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Sışlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi ÖzetPasteurella multocida (P. multocida) kuşlarda kanatlı kolerasına neden olur ve hastalıkta virulans faktörleri hala tam olarak anlaşılamamıştır. TonB proteini Pasteurella da dahil pek çok Gram-negatif bakteride yaygın olarak bulunan periplazmik bir protein olup membran dışına ferrik demir komplekslerini taşımakta görevlidir ve bakterilerde besin sağlamaya yardım etmek suretiyle önemli bir virulans faktörü olarak rol oynar. Bu çalışmada, 172 klinik solunum hastalıklı ördek ve kazdan toplam 23 P. multocida izolatı elde edilerek %13.4'lük izolasyon oranı sağlandı. P. multocida Serogrup A 22 vakadan izole edilirken (%95.7), serogrup F 1 vakadan izole edildi (%4.3). Tüm izolatlar 15 antibiyotiğe kar...

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Section: Discussionsupporting

confidence: 77%

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

Li¹,

Cheng²,

Lan³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…gallicida are also sometimes isolated (223). Capsular serotype A (mainly A:1, A:3, and A:4) is highly correlated to disease predilection for strains associated with fowl cholera (105,(224)(225)(226)(227), although capsular serotypes F and D have also been reported (123,124,227,228). Serotype B:3 is often isolated from avian disease cases that manifest as sinusitis (229,230) with symptoms of nasal discharge, increased lacrimation, swelling, and inflammation.…”

Section: Pasteurellosis and Fowl Choleramentioning

confidence: 99%

“…4) and has been used as a basis for serotyping (106,118). As a consequence, the cap loci of previously untyped P. multocida strains can now be subtyped based on clustering with the corresponding loci of known serotypes (118,(123)(124)(125).…”

Section: Detection Identification and Typing Of P Multocidamentioning

confidence: 99%

“…P. multocida is a common commensal or opportunistic pathogen found in the upper respiratory tracts of most livestock, domestic, and wild animals (34), including chickens (126)(127)(128)(129)(130)(131), turkeys (132,133), and other wild birds (123,(134)(135)(136)(137)(138)(139)(140)(141)(142)(143)(144), cattle and bison (121,(145)(146)(147), swine (34, 148-151), rabbits (152)(153)(154), dogs (41, [155][156][157], cats (domestic house cats as well as large wild cats, such as tigers, leopards, cougars, and lions) (39, 42-46, 49, 157-166), goats (125,139,167,168), chimpanzees (169), marine mammals (seals, sea lions, and walruses) (170), and even komodo dragons (171,172). The manifestation and pathological symptoms associated with Pasteurella infection, or "pasteurellosis," range from asymptomatic or mild chronic upper respiratory inflammation to acute, often fatal, pneumonic and/or disseminated disease.…”

Section: Pasteurella Disease In Animals Pasteurellosis Prevalencementioning

confidence: 99%

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Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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SUMMARY In a world where most emerging and reemerging infectious diseases are zoonotic in nature and our contacts with both domestic and wild animals abound, there is growing awareness of the potential for human acquisition of animal diseases. Like other Pasteurellaceae , Pasteurella species are highly prevalent among animal populations, where they are often found as part of the normal microbiota of the oral, nasopharyngeal, and upper respiratory tracts. Many Pasteurella species are opportunistic pathogens that can cause endemic disease and are associated increasingly with epizootic outbreaks. Zoonotic transmission to humans usually occurs through animal bites or contact with nasal secretions, with P. multocida being the most prevalent isolate observed in human infections. Here we review recent comparative genomics and molecular pathogenesis studies that have advanced our understanding of the multiple virulence mechanisms employed by Pasteurella species to establish acute and chronic infections. We also summarize efforts being explored to enhance our ability to rapidly and accurately identify and distinguish among clinical isolates and to control pasteurellosis by improved development of new vaccines and treatment regimens.

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“…Debilitated birds take higher risks while foraging, which improves their chances of getting caught or injured (Wobeser, 2006). Interpretation of the results is further complicated, as mycoplasma in diseased birds are often found together with other pathogenic organisms (Bezjian & Bezjian, 2014;Pennycott et al, 2005;Strugnell et al, 2011). The mycoplasma in these cases could therefore be the primary cause, co-infection, secondary infection or an incidental finding.…”

mentioning

confidence: 99%

Description and prevalence of Mycoplasma ciconiae sp. nov. isolated from white stork nestlings (Ciconia ciconia)

Palau-Ribes

Enderlein

Hagen

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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The mycoplasma strain ST 57 T was isolated from the trachea of a clinically healthy, free-ranging white stork nestling in Nielitz, Mecklenburg-Western Pomerania, Germany. Strain ST 57 T grew in fried-egg-shaped colonies on mycoplasma (SP4) agar plates and was dependent on sterol for growth. The organism fermented glucose and did not hydrolyse arginine or urea. The optimal growth temperature was 37 C, with a temperature range from 23 to 44 C. Strain ST 57 T could not be identified as a representative of any of the currently described mycoplasma species by alignment of the 16S rRNA gene sequence or 16S-23S intergenic transcribed spacer region, or by immunobinding assays. Thus, this organism appears to be a representative of a novel species, for which the name Mycoplasma ciconiae sp. nov. is proposed. The type strain is ST 57. Four further strains of this species are included in this description (ST 24=DSM 29908, ST 56 Clone 1=DSM 29054, ST 99=DSM 29909, ST 102=DSM 29010). The prevalence of this mycoplasma species in clinically healthy, white stork nestlings in northern Germany was determined. Our species-specific PCR detected 57.8 % (48/83) of the samples positive for M. ciconiae sp. nov. As this species appears to be widespread in the healthy free-ranging white stork population, we conclude that this species is either apathogenic or an opportunistic pathogen in white storks.

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Investigations into an outbreak of corvid respiratory disease associated withPasteurella multocida

Cited by 13 publications

References 25 publications

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

Çin’de Kanatlı Hayvanlardan İzole Edilen Pasteurella multocida Suşlarının Moleküler Karakterizasyonu ve tonB Geni Açısından Genetik Analizi

Pasteurella multocida: from Zoonosis to Cellular Microbiology

Description and prevalence of Mycoplasma ciconiae sp. nov. isolated from white stork nestlings (Ciconia ciconia)

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