Investigation on Various Aboveground Traits to Identify Drought Tolerance in Cowpea Seedlings

Ravelombola, Waltram; Shi, Ainong; Qin, Jun; Wang, Yuejin; Bhattarai, Gehendra; Zia, Bazgha; Zhou, Wei; Mou, Beiquan

doi:10.21273/hortsci13278-18

Cited by 19 publications

(28 citation statements)

References 22 publications

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“…Change in plant growth habit, tolerance index for plant maturity, tolerance index for flowering time, tolerance index for 100-seed weight, and tolerance index for grain yield were evaluated to quantify the relative tolerance to drought stress of the MAGIC cowpea population used for this study. Tolerance index has been used for efficiently assessing plant stress tolerance in previous studies 25 , 26 . Our results indicated large variation in tolerance index trait among the cowpea genotypes evaluated in this study, suggesting that this population is genetically diverse and could be used to enhance drought tolerance in a cowpea breeding program.…”

Section: Discussionmentioning

confidence: 99%

“…They conducted GWAS for flowering time, plant maturity, plant growth habit, 100-seed weight, and grain yield under full irrigation and restricted irrigation, respectively. In this study, we improve their analysis by assessing the drought tolerance of each individual within the cowpea MAGIC population using the tolerance index formula 25 , 26 . The GWAS was reanalyzed based on tolerance indices.…”

Section: Discussionmentioning

confidence: 99%

“…GWAS provides a greater mapping resolution over QTL mapping and efficiently permits the discovery of new genes 23 , 24 . However, the drought tolerance index trait, which is the relative change of the trait values due to drought stress 25 , 26 , was not investigated in this MAGIC cowpea population. Investigating the genetic architecture of the drought tolerance indices could lead to the discovery of new significant loci associated with drought tolerance in cowpea.…”

Section: Introductionmentioning

confidence: 99%

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Loci discovery, network-guided approach, and genomic prediction for drought tolerance index in a multi-parent advanced generation intercross (MAGIC) cowpea population

2021

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Cowpea is a nutrient-dense legume that significantly contributes to the population’s diet in sub-Saharan Africa and other regions of the world. Improving cowpea cultivars to be more resilient to abiotic stress such as drought would be of great importance. The use of a multi-parent advanced generation intercross (MAGIC) population has been shown to be efficient in increasing the frequency of rare alleles that could be associated with important agricultural traits. In addition, drought tolerance index has been reported to be a reliable parameter for assessing crop tolerance to water-deficit conditions. Therefore, the objectives of this study were to evaluate the drought tolerance index for plant growth habit, plant maturity, flowering time, 100-seed weight, and grain yield in a MAGIC cowpea population, to conduct genome-wide association study (GWAS) and identify single nucleotide polymorphism (SNP) markers associated with the drought tolerance indices, to investigate the potential relationship existing between the significant loci associated with the drought tolerance indices, and to conduct genomic selection (GS). These analyses were performed using the existing phenotypic and genotypic data published for the MAGIC population which consisted of 305 F8 recombinant inbred lines (RILs) developed at University of California, Riverside. The results indicated that: (1) large variation in drought tolerance indices existed among the cowpea genotypes, (2) a total of 14, 18, 5, 5, and 35 SNPs were associated with plant growth habit change due to drought stress, and drought tolerance indices for maturity, flowering time, 100-seed weight, and grain yield, respectively, (3) the network-guided approach revealed clear interactions between the loci associated with the drought tolerance traits, and (4) the GS accuracy varied from low to moderate. These results could be applied to improve drought tolerance in cowpea through marker-assisted selection (MAS) and genomic selection (GS). To the best of our knowledge, this is the first report on marker loci associated with drought tolerance indices in cowpea.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Loci discovery, network-guided approach, and genomic prediction for drought tolerance index in a multi-parent advanced generation intercross (MAGIC) cowpea population

2021

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“…A total of 36 Arkansas cowpea lines were used in this study for drought-tolerance evaluation. Also, two previous reported drought-tolerant lines 'PI293568' and 'PI349674', and one drought-susceptible line, 'PI255774' (Ravelombola et al, 2018) were used as controls (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Drought Tolerance in Arkansas Cowpea Lines at Seedling Stage

Cui¹,

Xiong²,

Yufeng³

et al. 2020

horts

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Cowpea [Vigna unguiculate (L.) Walp.] is not only a healthy, nutritious, and versatile leguminous crop; it also has a relatively high adaptation to drought. Research has shown that cowpea lines have a high tolerance to drought, and many of them can survive more than 40 days under scorching and dry conditions. The cowpea (Southern pea) breeding program at the University of Arkansas has been active for more than 50 years and has produced more than 1000 advanced breeding lines. The purpose of this study was to evaluate the drought-tolerant ability in Arkansas cowpea lines and use the drought-tolerant lines in cowpea production or as parents in cowpea breeding. A total of 36 University of Arkansas breeding lines were used to screen drought tolerance at the seedling stage in this study. The experiment was conducted in the greenhouse using a randomized complete block design (RCBD) with two replicates, organized in a split-plot manner, where the drought treatment (drought and nondrought stress) as the main plot and the cowpea genotypes as the subplot. Drought stress was applied for 4 weeks, and three drought-tolerant–related traits were collected and analyzed. Results showed that cowpea breeding lines: ‘17-61’, ‘17-86’, ‘Early Scarlet’, and ‘ARBlackeye #1’ were found to be drought tolerant.

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“…Both groups had accessions 835-911, IT07-292-10, IT90-59, IT89KD288, Chibundi mavara, and TVU12746, which were tolerant to drought, while RV344 and Kangorongondo were susceptible to drought during the first week of drought imposition.Pungulani et al (2012) also observed significant variations for 36 accessions that were characterised for canopy maintenance in a glasshouse. Only five accessions had apical re-growth, high relative water content, stem greenness and lower scores for both leaf wilting scales and leaf wilting index after the first week of stress at a soil moisture content of 2.9% while another five cowpea accessions showed high levels of drought susceptibility Ravelombola et al (2018). found large variations with three cowpea accessions that tolerant to wilting and drought on 30 cowpea accessions that were screened for droughtrelated traits at seedling stage in a greenhouse.From this experiment, positive correlations were observed on stem greenness from week one up to week three as well as yield related traits such as seed weight (SWT), pod length (PL), pod weight (PWT), number of pods (NP) and seeds per pod (SP).…”

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Phenotyping cowpea accessions at the seedling stage for drought tolerance using the pot method

Nkomo

2020

Preprint

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One of the most important screening techniques used in cowpea selection for drought tolerance is screening at the seedling stage. The objective of this study was to phenotype 60 cowpea genotypes for seedling drought tolerance in screen houses (glasshouse and greenhouse). A triplicated 6 × 10 alpha lattice design with four blocks was used for the experiments. After planting, pots were watered to field capacity, thereafter watering was completely withheld for 4 weeks after planting (WAP), when plants were at the three-leaf stage. Principal component analysis revealed that of the 14 variables, the first four expressed more than 1 eigenvalue. Data showed that PC1, PC2, and PC3 contributed 39.3%, 15.2%, and 10% respectively, with 64.68% total variation. Bartlett’s test of sphericity was significant at p<0.05, while the Kaiser-Meyer-Olkin measure of sampling adequacy was 77. A PCA plot and biplot showed that the number of pods (NP), seeds per pod (SP), survival count (SC), pod weight (PWT), and stem wilting in week one (WWK1) had the most significant contributions to genetic variability to drought tolerance and to yield after stress imposition Based on the PCA, biplot, and cluster plot, the accessions IT 07-292-10, IT 07-274-2-9, IT90K-59, 835-911, RV 343, and IT 95K-2017-15 had the maximum variability in terms of number of pods, seeds per pod, survival count, pod weight and wilting in week one after drought imposition. Cowpea accessions 835-911, IT 07-292-10, RV 344, Kangorongondo, and IT 90K-59 were the major individuals that contributed mainly to domain information model (DIM) 1 and 2. The accessions that contributed the least were IT 89KD288, Chibundi mavara, and TVU12746. Thirty-six cowpea accessions from both screen houses were tolerant to drought, 15 were moderately tolerant, while 23 were susceptible. The findings of the study provided a useful tool for screening and determining drought-tolerant and susceptible accessions at the seedling stage. Thirty-six cowpea accessions from both screen houses were tolerant to drought as well as those that showed great variability can be used as parents in future cowpea breeding programmes.

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Investigation on Various Aboveground Traits to Identify Drought Tolerance in Cowpea Seedlings

Cited by 19 publications

References 22 publications

Loci discovery, network-guided approach, and genomic prediction for drought tolerance index in a multi-parent advanced generation intercross (MAGIC) cowpea population

Loci discovery, network-guided approach, and genomic prediction for drought tolerance index in a multi-parent advanced generation intercross (MAGIC) cowpea population

Evaluation of Drought Tolerance in Arkansas Cowpea Lines at Seedling Stage

Phenotyping cowpea accessions at the seedling stage for drought tolerance using the pot method

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