Investigation on the interaction of pyrene with bovine serum albumin using spectroscopic methods

Xu, Chao; Gu, Jie; Ma, Xiaoli; Dong, Tian; Meng, Xianmin

doi:10.1016/j.saa.2014.01.132

Cited by 51 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Förster's non‐radiative energy‐transfer theory, the distance( r ) between the donor and the acceptor, critical energy‐transfer distance R 0 when the transfer efficiency is 50% and the energy‐transfer efficiency E can be calculated by the formulas :

E = 1 - F true/ F_{0} = R_{0}^{6} true/ ((), R_{0}^{6} + r^{6})

R_{0}^{6} = 8.79 \times 10^{prefix- 25} K^{2} normalΦ N^{prefix- 4} J

J = true \sum F ((), λ) ε ((), λ) λ^{4} normalΔ λ true/ true \sum F ((), λ) normalΔ λ

where, F 0 is the fluorescence intensity of the donor, F is the fluorescence intensity for the donor in the presence of the same concentration of acceptor; K 2 is the orientation factor, Φ is the fluorescence quantum yield of the donor in the absence of acceptor, N is the refractive index of the medium; F ( λ ) is the fluorescence intensity of the fluorescence donor at wavelength λ and ε ( λ ) is the molar absorption coefficient of the acceptor at this wavelength. Under these experimental conditions, it has been reported previously that K 2 = 2/3, N = 1.336 and Φ = 0.118 . J is the overlapping area.…”

Section: Resultsmentioning

confidence: 83%

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Liu

Zhang

et al. 2015

Luminescence

View full text Add to dashboard Cite

The reaction mechanism of cefoxitin sodium with bovine serum albumin was investigated using fluorescence spectroscopy and synchronous fluorescence spectroscopy at different temperatures. The results showed that the change of binding constant of the synchronous fluorescence method with increasing temperature could be used to estimate the types of quenching mechanisms of drugs with protein and was consistent with one of fluorescence quenching method. In addition, the number of binding sites, type of interaction force, cooperativity between drug and protein and energy-transfer parameters of cefoxitin sodium and bovine serum albumin obtained from two methods using the same equation were consistent. Electrostatic force played a major role in the conjugation reaction between bovine serum albumin and cefoxitin sodium, and the type of quenching was static quenching. The primary binding site for cefoxitin sodium was sub-hydrophobic domain IIA, and the number of binding sites was 1. The value of Hill's coefficients (nH ) was approximately equal to 1, which suggested no cooperativity in the bovine serum albumin-cefoxitin sodium system. The donor-to-acceptor distance r < 7 nm indicated that static fluorescence quenching of bovine serum albumin by cefoxitin sodium was also a non-radiation energy-transfer process. The results indicated that synchronous fluorescence spectrometry could be used to study the reaction mechanism between drug and protein, and was a useful supplement to the conventional method. Copyright © 2015 John Wiley & Sons, Ltd.

show abstract

E = 1 - F true/ F_{0} = R_{0}^{6} true/ ((), R_{0}^{6} + r^{6})

R_{0}^{6} = 8.79 \times 10^{prefix- 25} K^{2} normalΦ N^{prefix- 4} J

J = true \sum F ((), λ) ε ((), λ) λ^{4} normalΔ λ true/ true \sum F ((), λ) normalΔ λ

Section: Resultsmentioning

confidence: 83%

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Liu

Zhang

et al. 2015

Luminescence

View full text Add to dashboard Cite

show abstract

“…In not presented investigations we could observe a phase transition already in PBS and also in PBS/DMEM without PS and FBS, however the sharp reversible phase transition was only monitored in the presence of FBS. Consequently the main component affecting the thermoresponsive behaviour of the pyrene functionalized polymers is BSA, which was shown in a study of Xu et al . to bind to pyrene.…”

Section: Methodsmentioning

confidence: 99%

Fluorescent Polyvinylphosphonate Bioconjugates for Selective Cellular Delivery

Schwarzenböck

Schäffer

Nößner

et al. 2018

Chemistry A European J

View full text Add to dashboard Cite

To date, many poly(ethylene glycol) (PEG) and poly(N-isopropylacrylamide) (PNIPAAm) biomolecule conjugates have been described, but they often show long response times, are not bio-inert, or lose function in biological fluids. Herein, we present a modular synthetic approach to generate polyvinylphosphonate biomolecule conjugates. These conjugates exhibit a sharp phase transition temperature even under physiological conditions where few other examples with this property have been described to date. Furthermore, it was feasible to add biological functions to the polymers via the conjugation step. The polyvinylphosphonate cholesterol constructs are attached to the cellular membrane and the folic acid anchored polymers are shuttled into the cells. This is an exceptional finding through a straightforward synthetic approach.

show abstract

“…The CD spectra result was showed in Fig.16. It can be seen that the BHb in buffer solution (pH 6.04, 0.2M) displayed two negative peaks at 208nm and 220nm, which are the characteristic of α-helical structure of protein [49]. Compared with the BHb in buffer solution, the BHb eluted from the MIP by 10% SDS (w/v)-10% HAc (v/v) showed a slightly different CD spectrum, indicating that the conformation of BHb was changed during the process of desorption.…”

Section: Regeneration and Stabilitymentioning

confidence: 99%

Magnetic-graphene based molecularly imprinted polymer nanocomposite for the recognition of bovine hemoglobin

Jun

Wang

Liu

et al. 2015

Talanta

View full text Add to dashboard Cite

Investigation on the interaction of pyrene with bovine serum albumin using spectroscopic methods

Cited by 51 publications

References 32 publications

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Fluorescent Polyvinylphosphonate Bioconjugates for Selective Cellular Delivery

Magnetic-graphene based molecularly imprinted polymer nanocomposite for the recognition of bovine hemoglobin

Contact Info

Product

Resources

About