Investigation on the inhibition mechanism and binding behavior of cryptolepine to α-glucosidase and its hypoglycemic activity by multi-spectroscopic method

Min, Xiaofeng; Guo, Suqin; Lu, Yujing; Xu, Xuetao

doi:10.1016/j.jlumin.2024.120437

Cited by 12 publications

(4 citation statements)

References 46 publications

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“…To the 3.0 mL of tyrosinase solution, 1 μL of quercetagetin was added by titration method ( Li et al, 2024a ; Min et al, 2024 ). The mixture was measured fluorescence spectra at excitation of 280 nm.…”

Section: Methodsmentioning

confidence: 99%

Inhibition mechanism investigation of quercetagetin as a potential tyrosinase inhibitor

Liang

2024

Front. Chem.

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Tyrosinase is one important rate limiting enzyme in melanin synthesis, directly affecting the melanin synthesis. Quercetagetin is one active ingredient from marigold. Thence, the inhibition effects of quercetagetin against tyrosinase were investigated. The results showed quercetagetin could inhibit tyrosinase activity with IC50 value of 0.19 ± 0.01 mM and the inhibition type was a reversible mixed-type. Results of fluorescence quenching showed quercetagetin could quench tyrosinase fluorescence in static process. CD and 3D fluorescence results showed the interaction of quercetagetin to tyrosinase could change tyrosinase conformation to inhibit activity. Moreover, docking revealed details of quercetagetin’s interactions with tyrosinase.

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Section: Methodsmentioning

confidence: 99%

Inhibition mechanism investigation of quercetagetin as a potential tyrosinase inhibitor

Liang

2024

Front. Chem.

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“…NOB has healthcare functions due to its profound bioactivities, such as anticancer, anti-inflammation, anti-atherosclerosis, anti-viral, and neuroprotective effects, and its ability to prevent neurodegenerative diseases and regulate lipid metabolism, glucose metabolism, mitochondrial function, and muscle physiology ( Gao et al, 2018 ; Nakajima and Ohizumi, 2019 ; Nohara et al, 2019 ). These bioactivities can be achieved by the blood circulation system in vitro ( Chen et al, 2020 ; Min et al, 2024 ). In vivo experiments infer that NOB and its metabolites are distributed to different tissues via the blood circulation system and ultimately excreted in urine.…”

Section: Introductionmentioning

confidence: 99%

Natural product nobiletin-loaded Pickering emulsion stabilized by bovine serum albumin/carboxymethyl inulin complexes: preparation and digestive characteristics

Huang,

Zhang,

Feng

et al. 2024

Front. Pharmacol.

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To expand the application of nobiletin (NOB) in semi-solid functional foods, bovine serum albumin (BSA)/carboxymethyl inulin (CMI) complexes-stabilized Pickering emulsion (BCPE) (φoil = 60%, v/v) was fabricated, and the swallowing index and bioavailability of the NOB-loaded Pickering emulsion was evaluated. Confocal laser scanning microscope (CLSM) and cryo-scanning electron microscopy (cryo-SEM) images revealed that BSA/CMI complexes attached to the oil–water interface. NOB-loaded BCPE exhibited a viscoelastic and shear-thinning behavior. Fork drip test results suggested that the textural value of unloaded and NOB-loaded emulsions was International Dysphagia Diet Standardisation Initiative Level 4, which could be swallowed directly without chewing. The in vitro lipolysis model suggested that NOB had a faster digestive profile and a higher bioaccessibility in the BCPE than in the oil suspension. The in vivo rat model revealed that the oral bioavailability of NOB was increased by 2.07 folds in BCPE compared to its bioavailability in unformulated oil. Moreover, BCPE led to a higher plasma concentration of the major demethylated metabolite of NOB (4′-demethylnobiletin) than the unformulated oil. Accordingly, BCPE enhanced the oral bioavailability of NOB by improving bioaccessibility, absorption, and biotransformation.

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“…More and more evidences have suggested that diabetes is a multifactorial disease accompanied by the dysregulation of lipid and glucose metabolism, and oxidative stress, which plays an important role in the occurrence and development of diabetes ( Maria and Mattias, 2017 ; Hu et al, 2020 ; Sourbh and Jeena, 2022 ). The long-term hyperglycemia in patients with diabetes may lead to chronic injury and dysfunction of various tissues ( Satman et al, 2023 ; Min et al, 2024 ). Although the commonly used hypoglycemic clinical agents, such as metformin and glibenclamide can lower blood glucose, they often induce a range of side effects including diarrhea, intestinal flatulence, hemolytic anemia, lactic acidosis and congestive heart failure ( Xing et al, 2021 ; Elkhalifa et al, 2024 ).…”

Section: Introductionmentioning

confidence: 99%

N-p-coumaroyloctopamine ameliorates hepatic glucose metabolism and oxidative stress involved in a PI3K/AKT/GSK3β pathway

Huang,

Zhang,

et al. 2024

Front. Pharmacol.

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Type 2 diabetes mellitus is regarded as a chronic metabolic disease characterized by hyperglycemia. Long-term hyperglycemia may result in oxidative stress, damage pancreatic β-cell function and induce insulin resistance. Herein we explored the anti-hypoglycemic effects and mechanisms of action of N-p-coumaroyloctopamine (N-p-CO) in vitro and in vivo. N-p-CO exhibited high antioxidant activity, as indicated by the increased activity of SOD, GSH and GSH-Px in HL-7702 cells induced by both high glucose (HG) and palmitic acid (PA). N-p-CO treatment significantly augmented glucose uptake and glycogen synthesis in HG/PA-treated HL-7702 cells. Moreover, administration of N-p-CO in diabetic mice induced by both high-fat diet (HFD) and streptozotocin (STZ) not only significantly increased the antioxidant levels of GSH-PX, SOD and GSH, but also dramatically alleviated hyperglycemia and hepatic glucose metabolism in a dose-dependent manner. More importantly, N-p-CO upregulated the expressions of PI3K, AKT and GSK3β proteins in both HG/PA-induced HL-7702 cells and HFD/STZ-induced mice. These findings clearly suggest that N-p-CO exerts anti-hypoglycemic and anti-oxidant effects, most probably via the regulation of a PI3K/AKT/GSK3β signaling pathway. Thus, N-p-CO may have high potentials as a new candidate for the prevention and treatment of diabetes.

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Investigation on the inhibition mechanism and binding behavior of cryptolepine to α-glucosidase and its hypoglycemic activity by multi-spectroscopic method

Cited by 12 publications

References 46 publications

Inhibition mechanism investigation of quercetagetin as a potential tyrosinase inhibitor

Inhibition mechanism investigation of quercetagetin as a potential tyrosinase inhibitor

Natural product nobiletin-loaded Pickering emulsion stabilized by bovine serum albumin/carboxymethyl inulin complexes: preparation and digestive characteristics

N-p-coumaroyloctopamine ameliorates hepatic glucose metabolism and oxidative stress involved in a PI3K/AKT/GSK3β pathway

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