Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L.

El-Ghamery, Abbas A.; Mousa, Mohamed

doi:10.1016/j.aoas.2016.11.002

Cited by 26 publications

(9 citation statements)

References 33 publications

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“…This hormones remain the most predominantly used cytokinin in in vitro plant tissue culture and in vivo agricultural applications to stimulate and synchronise plant processes such as flowering, fruiting and biomass allocations (Mansour et al, 2014). This hormone has been widely reported to significantly increase fresh and dry biomass fractions of leaves, roots and stems in many plant species including rice, spurges and soybean (Saadawy and Abdel-Moniem, 2015;Mangena et al, 2015;El-Ghamery and Mousa, 2017). Notwithstanding the larger number of reports on the effect of cytokinin application on the growth and in vitro regeneration of legumes, the effects of these hormones on the biomass, growth and yield of soybean have Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced.... not been well documented.…”

Section: Introductionmentioning

confidence: 99%

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

Mangena

2020

IJARe

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Seed priming has potential to improve seedling development and plant growth under environmental stress. In this study, seeds of soybean cultivar LS678 and TGx1835-10E were pretreated with an optimum level of benzyladenine (4.87 mgL-1) before sowing into pots containing pasteurised mixture of vermiculite and sand. Plants were grown up to V3 stage before exposure to moderate and severe drought stress. According to the results, germination was rapid in hydroprimed seeds than BA primed seeds, which took longer to emerge. However, growth, yield and biomass of BA primed plants were increased (number of branches per plant- 7.32, flowering-87.6%, 100 seed weight- 22.6 g, overall biomass fraction- >40.5%) compared to plants developed from hydroprimed seeds (number of branches- 3.61, seed weight- 19.2 g, biomass- 12%) under similar growth conditions. This study indicated that, hormonal seed priming with BA reasonably enhanced soybean growth, particularly root biomass, flowering and fruiting. These effects further suggest that BA may play a significant role in improving drought tolerance in soybean.

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Section: Introductionmentioning

confidence: 99%

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

Mangena

2020

IJARe

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“…Chromosome laggards may also cause micronucleus formation (Leme and Marin-Morales 2009). Stickiness which leads to DNA-DNA or DNA-protein crosslinking may possibly be caused by the depolymerization of DNA or partial dissolution of the nucleoproteins or the chromosomal intercalation of the chromatin fibers (Fiskesjö and Levan 1993;Türkoğlu 2015;El-Ghamery and Mousa 2017). Anaphase bridge may occur due to the clastogenic effect of the chemicals such as breakage or fusion of chromosomes, unequal chromatid exchange, dicentric chromosome formation, alteration of the activation of replication enzymes or incomplete adhesion (El-Ghamery et al 2000;Luo et al 2004;Bonciu et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and genotoxic assessment of 2-chloropyridine using Allium cepa ana-telophase and comet test

Pirdal

Liman

2019

Mediterranean Agricultural Sciences

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2-Chloropyridine (2-CPY) is an important precursor of cosmetics, pesticides and other pharmaceutical products and it is also defined as trace chemical in industrial wastewater as the products of the metabolites of agricultural chemical products and river pollutants. In this study, the effects of 2-CPY on mitotic index (MI), mitotic phase frequencies, chromosome aberrations (CAs) and DNA damage in Allium cepa root cells were investigated with Allium ana-telophase and comet assay. Concentrations of 0.5xEC50 (25 ppm), EC50 (50 ppm) and 2xEC50 (100 ppm) of 2-CPY, Methyl methanesulfonate (MMS-10 ppm, positive control) and distilled water (negative control) were applied to A. cepa roots for 24, 48, 72 and 96 h. 2-CPY showed a cytotoxic effect by reducing root growth and MI, but also showed genotoxic effect by increasing CAs (disturbed ana-telophase, chromosome laggards, stickiness, bridges and polyploidy) and DNA damage at substantial levels. The amount of 2-CPY was shown to be increased statistically in both duration and dose by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 2-CPY should be used carefully and investigated its cytogenotoxic effects with other toxicology test systems.

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“…Chromosome stickiness may result from entanglement of chromatin fibers, which fall to condense properly in preparation for cell division [43] [44] [45]. Stickiness can cause serious changes in nucleic acids physico-chemical properties or chromatin condensation of the nucleus [46]. Also, sticky chromosomes can cause loss of genetic material and subsequently, the cell-division process occurs irregularly, with some chromosomes not adhering to the assembled chromosomal complex and being lost during the cell cycle.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

Roșculete¹,

Olaru²,

Roșculete³

et al. 2020

AJPS

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With the unprecedented development of the food industry, food preservatives have gained a leading role in food processing. In this study, investigations were carried out to assess the cytological effects of potassium metabisulphite (PMB) to onion (Allium cepa), one of the most used plants for determining the cytotoxic and genotoxic effects of different chemicals. Meristematic roots of A. cepa were treated with PMB solutions in different concentrations, ranging from 10 mg/l to 35 mg/l for 6, 12 and 24 h alongside an untreated control. The quantified parameters for the different concentrations of the PMB were mitotic index (MI %), mitosis phases index (PI %) and total abnormalities index (TAI %) meaning chromosomal aberrations and nuclear abnormalities. The results indicated that PMB reduced MI in A. cepa with increasing the concentrations and time exposure as compared with the untreated control. Thus, at concentration of 10 -35 mg/l PMB, these values were reduced from 8.34% to 4.81% (6 h); 7.18% to 2.35% (12 h) and 4.12 to 1.43 (24 h). Also, the TAI value increased with increasing PMB concentrations and time: 0.51% to 9.98% (6 h), 7.11% to 19.84% (12 h) and 17.79 to 41.21 (24 h). The types of abnormalities induced by PMB in A. cepa meristematic cells were micronucleus, C-metaphase, star anaphase, stickiness, laggards, fragments, binucleated cells and pulverised nucleus. These alarming findings indicate the cytotoxic and genotoxic effect of PMB to A. cepa and suggest necessity to adopt more natural alternatives for food preservation in the future.

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Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L.

Cited by 26 publications

References 33 publications

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

Cytotoxic and genotoxic assessment of 2-chloropyridine using Allium cepa ana-telophase and comet test

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

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Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L.

Cited by 26 publications

References 33 publications

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

Cytotoxic and genotoxic assessment of 2-chloropyridine using Allium cepa ana-telophase and comet test

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to &lt;i&gt;Allium cepa&lt;/i&gt;

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Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>