Investigation on the biofilm eradication potential of selected medicinal plants against methicillin-resistant Staphylococcus aureus

Prabu, S.; Umamaheswari, A; Felciya, Sekar Jeyakumar Grace

doi:10.1016/j.btre.2020.e00523

Cited by 8 publications

(3 citation statements)

References 49 publications

(48 reference statements)

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“…The ethanolic extracts were employed for preliminary screening of phytochemicals such as alkaloids, anthocyanins, anthraquinones, cardiac glycosides, coumarins, flavonoids, phenols, saponins, steroids, tannins, and terpenoids following the standardized protocols [5,13,[20][21][22]. The results were expressed as (+) for the presence and (-) for the absence of phytochemical compounds.…”

Section: Phytochemical Screeningmentioning

confidence: 99%

“…The multiple drug resistance mechanisms include drug uptake limitation, drug target modification, drug inactivation, and active drug efflux, and the resistance processes differ based on microbial types [ 4 ]. Besides, certain pathogenic bacteria form biofilms through quorum sensing and develop drug resistance [ 5 ]. Hence, there is a need for the discovery of new drugs against multidrug-resistant pathogenic microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…Plant extracts and their bioactive compounds have been utilized for the treatment of several diseases since ancient times [ 5 ]. Medicinal plants were the major sources of bioactive compounds that could be used as potential alternatives to conventional antimicrobials [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia

Anbessa,

Lulekal,

Hymete

et al. 2024

BMC Complement Med Ther

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Background Medicinal plants play a major role in the delivery of healthcare, particularly among the rural population of Ethiopia. Plant extracts and their bioactive compounds have been utilized for the treatment of several diseases. This study was aimed at evaluating the antibacterial activity, antioxidant capacity, and phytochemical content of selected medicinal plants used in Dibatie district, western Ethiopia. Methods Study plants were collected, shade dried, pulverized, extracted by maceration in 80% ethanol, and subjected to antibacterial, antioxidant, and phytochemical tests. Minimum inhibitory concentration (MIC) was determined using 96-well microplates and nutrient broth microdilution. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Phytochemical screening was conducted using standard test methods. Results The ethanolic extract of Polystachya steudneri Rchb.f. pseudobulbs was the most active against gram-negative Proteus mirabilis, Salmonella typhimurium, Klebsiella pneumoniae, Escherichia coli, and Shigella flexneri, with MIC values of 8 ± 0, 11 ± 5, 3 ± 1, 3 ± 1, and 2 ± 0 mg/mL, respectively. The ethanolic extract of P. steudneri was also the most effective against gram-positive Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, and Enterococcus faecalis, with MIC values of 8 ± 0, 8 ± 0, 3 ± 1, and 16 ± 0 mg/mL, respectively. Ethanolic extracts of Gnidia involucrata Steud. ex A.Rich. stems and roots were effective antioxidants, with respective 50% DPPH free radical inhibitory concentrations (IC50) of 168.68 and 181.79 µg/mL, followed by that of P. steudneri (IC50 = 203.11 µg/mL). The study plants contained alkaloids, anthocyanins, anthraquinones, cardiac glycosides, coumarins, flavonoids, phenols, saponins, steroids, tannins, and terpenoids. Conclusions This study confirmed the antibiotic, antioxidant, and phytochemical constituents of the investigated plants and suggested further investigations that may lead to bioactive lead compounds.

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Section: Phytochemical Screeningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia

Anbessa,

Lulekal,

Hymete

et al. 2024

BMC Complement Med Ther

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Antimicrobial and antibiofilm effect of cannabinoids from Cannabis sativa against methicillin-resistant Staphylococcus aureus (MRSA) causing bovine mastitis

Roshan,

Singh,

Vats

et al. 2024

Int Microbiol

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Hybrid Chitosan/CaO-Based Nanocomposites Doped with Plant Extracts from Azadirachta indica and Melia azedarach: Evaluation of Antibacterial and Antibiofilm Activities

et al. 2022

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This study examines substances from plant leaves of Azadirachta indica and Melia azedarach extracted with methanol (80%) and then successively fractionated with increasing gradient polarity solvents. The methanol extract from the two plant leaves was selected to be incorporated with nanocomposites because of their high antimicrobial activities. HPLC analysis was performed for the determination of total phenolics and avonoids. LC-ESI-MS positive ion acquisition mode revealed the presence of four main chemical classes; limonoids, triterpenes, fatty acids, and phenolics. Sol-gel processes were adapted to prepare CaO/chitosan composite loaded with the methanol extract of the two plant leaves, acting as innovative antibacterial agents. The fabricated nanocomposites were characterized by XRD, TEM, SEM, and FT-IR methods. Minimal bactericidal concentrations (MBC) were established by agar plating using bacteria treated with MIC and at least 2 concentrations double-higher than the MIC. Bio lm was cultivated in the presence of sub-MICs of the extracts and the nanocomposites, followed by quanti cation with the crystal violet assay. The examined chitosan-CaO-based hybrid nanocomposites loaded with the methanol fractions from the leaves of A. indica and M. azedarach had an excellent performance as bio lm inhibitors. This indicates their good potential for biomedical applications.

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Investigation on the biofilm eradication potential of selected medicinal plants against methicillin-resistant Staphylococcus aureus

Abstract: Highlights Investigation of A. indica, M. oleifera, M. koenigii and P. guajava extracts against MRSA biofilm. Pet. ether extract of A. indica show superior antibiofilm activity. Extracts are non-toxic with minimal hemolytic properties.

Cited by 8 publications

References 49 publications

Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia

Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia

Antimicrobial and antibiofilm effect of cannabinoids from Cannabis sativa against methicillin-resistant Staphylococcus aureus (MRSA) causing bovine mastitis

Hybrid Chitosan/CaO-Based Nanocomposites Doped with Plant Extracts from Azadirachta indica and Melia azedarach: Evaluation of Antibacterial and Antibiofilm Activities

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