Investigation on Pollination Methods for the Intergeneric Hybrid Between Hemerocallis and Lilium

Reproductive barriers in interspecific hybridizations among Chimonanthus praecox (L.) Link, C. salicifolius S. Y. Hu, and C. nitens Oliver from pollen–pistil interaction and hybrid embryo development

“…This phenomenon was the main reason for the lack of seed setting by interspecific hybrids. The artificial culture in vitro of immature embryos at appropriate stages could effectively overcome embryo abortion-or dysplasia-induced hybrid seed problems related to the lack of an embryo or to seed germination difficulties; in this case, embryo age, culture media, and culture methods have important impacts on the success of hybrid embryo culture (Van Tuyl et al, 1991;Van Creij et al, 1999Ahn et al, 2003). Cheng et al (2010) employed ovary rescue to create interspecific hybrids between Chrysanthemum morifolium 'rm20-12' and its wild relative C. nankingense, whose cold tolerance was significantly superior to that of the parents.…”