Investigation on Chemical Composition, Antimicrobial, Antioxidant, and Cytotoxic Properties of Essential Oil From Dracocephalum Kotschyi Boiss

Ashrafi, Behnam; Ramak, Parvin; Ezatpour, Behrouz; Talei, Gholam Reza

doi:10.21010/ajtcam.v14i3.23

Cited by 24 publications

(7 citation statements)

References 28 publications

(23 reference statements)

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“…The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations were determined by the broth microdilution method in 96-well microtiter plates [25][26][27][28]. Briefly, bacterial strains were cultured overnight at 37 °C on MHB, adjusted to a final density of 8 log CFU/mL, and used to inoculate (1-10) 96-well microliter plates containing serial dilutions of the essential oils (5120-20 μg/mL) on MHB.…”

Section: Minimum Inhibition Concentration (Mic) and Minimal Bactericimentioning

confidence: 99%

Biological Activity and Chemical Composition of the Essential Oil of Nepeta cataria L.

Ashrafi¹,

Ramak²,

Ezatpour³

et al. 2019

jrp

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In this study, chemical composition, antioxidant and antimicrobial activity of the essential oil of Nepeta cataria L., a native plant that grows in Lorestan region of Iran, were studied. Chemical constituents of the essential oil were identified by means of gas chromatography/mass spectrometry (GC/MS) and the relative content of each constituent was determined by area normalization. Disc diffusion and broth microdilution assays were used for in vitro antimicrobial screening. Antioxidant activity was employed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging. The predominant bioactive ingredients with high percentage in N. Cataria essential oil was 4a-α, 7-α, 7a-β-Nepetalactone (53.87%). The essential oil's IC50 value of 80.62 μg / mL in the DPPH assay, could be regarded as its strong antioxidant potential. The antibacterial activities of the essential oil were screened and compared against dozen important human pathogens. The essential oil showed good antibacterial activity, especially on Salmonella spp.. The biggest diameters of inhibition zones (29 mm) was recorded for Salmonella typhi and Salmonella paratyphi A showed the lowest MIC (160 μg / mL).

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Section: Minimum Inhibition Concentration (Mic) and Minimal Bactericimentioning

confidence: 99%

Biological Activity and Chemical Composition of the Essential Oil of Nepeta cataria L.

Ashrafi¹,

Ramak²,

Ezatpour³

et al. 2019

jrp

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“…The major components were blumenol C (49.7%), β-cedrene (6.1%), limonene (3.8%), α-guaiene (3.3%), cryptone (3.1%), and α-cyperone (2.7%). The EO was tested for its cytotoxic activity against HepG2 and MCF-7 cells, with IC 50 values of 0.072 and 0.188 mg/mL, respectively [46].…”

Section: Origanummentioning

confidence: 99%

Cytotoxic Activity of Essential Oils of Some Species from Lamiaceae Family

Pérez-González¹,

Pérez‐Ramos²,

Méndez-Cuesta³

et al. 2019

Cytotoxicity - Definition, Identification, and Cytotoxic Compounds

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Cancer is considered one of the most lethal diseases in the world, with a prevalence of 439.2 cases and 163.5 deaths per 100,000 inhabitants, in the period from 2011 to 2015; this disease has a greater impact in underdeveloped countries. For the treatment of this disease, a combination of chemotherapy with surgery or radiation is generally used, however, it is not exempt from adverse effects or resistance of the tumor to this type of treatment, for this reason the search for new treatments is constant. The plants are a possible source to achieve this; Lamiaceae is a family of plants widely distributed on the planet and has been used traditionally for the treatment of different diseases, and various essential oils with the potential for cancer treatment have been isolated from this species. The scope of this review is to present 46 essential oils isolated from different species of Lamiaceae which have been tested against different cancer cell lines.

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“…This however leaves over 70% of the country's provinces. Ashrafi et al [8] observed that D. kotschyi is native in Iran and grows wild in regions lying between 2000 and 3200m altitudes. However, in recent times, D. kotschyi is reported to be an endangered plant in Iran following excessive harvesting, limited distribution areas, and a lack of cultivation and domestication of the D. kotschyi plant [42].…”

Section: Resultsmentioning

confidence: 99%

“…A number of researchers have presented various constituents of D. kotschyi , with the most frequently reported being limonene, luteolin, geranial, apigenin, and calycopterin. A comparison of the main components of D. kotschyi from different provinces is presented in Table 1 [8, 10, 11, 14, 17, 18, 23–25, 31, 39, 44, 45, 48–66]. In spite of some similarities in reported components, there are significant quantitative and qualitative differences between the samples obtained from different locations in Iran.…”

Section: Discussionmentioning

confidence: 99%

“…The plant has small pubescent leaves that are calyx two-lipped, with upper lip three-toothed, stamens, and flowers in verticillaster in the upper leaves [7, 8]. Dracocephalum kotschyi is enriched in various constituents of essential oils including citral, caryophyllene, terpinyl, acetate, limonene, α -terpineol, δ -3-carene, α -pinene, terpinen-4-ol, geranial, limonene-10-al, 1,1-dimethoxydecane, Gerania, α -pinene [9], and flavonoids such as calycopterin, xanthomicrol, isokaempferide, luteolin, apigenin, luteolin 7-O- β -D-glucopyranoside, luteolin 3′-O- β -D-glucuronide, apigenin 4′-O- β -D-glucopyranoside, acacetin 7-O- β -D-glucopyranoside [10–14], limonene-10-al, and limonene, among others [15].…”

Section: Introductionmentioning

confidence: 99%

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Medicinal Properties and Active Constituents of Dracocephalum kotschyi and Its Significance in Iran: A Systematic Review

Heydari

Yavari

Adibi

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Objectives. Dracocephalum has over 60 species and is found mainly in the temperate regions of Asia and Europe. One of these species, i.e., Dracocephalum kotschyi Boiss, is known to have a number of medicinal properties and active ingredients in many parts of the world. Despite being an endemic wild-flowering plant of great importance, the plant is currently considered endangered in Iran. Besides, there is paucity of information on the significance of the medicinal properties and active constituents of D. kotschyi among the Iranian people. On that account a systematic review of studies reporting on the medicinal properties and active ingredients and its significance to human and animal health was conducted and the existing knowledge gaps were identified. Methods. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used in the search for published articles on medicinal properties and active ingredients of D. kotschyi and its significance on humans and animals in Iran. The search was confined to scientific articles from repositories of popular data bases and search engines among them PubMed, Web of Science, Google Scholar, Science Direct, SpringerLink, and Scopus. The search narrowed down on scientific journals, books, and book chapters focusing on the medicinal properties of D. kotschyi in Iran for the period between 1970 and 2018. Results. A total of 1158 scientific articles were sourced from the various databases, out of which 38 met the search criteria and qualified for this review. The studies were conducted in only 9 of the 31 provinces of Iran, with a large proportion in Isfahan province, central Iran. The studies showed that all plant parts (roots, aerial parts, flowers, and leaves) had active constituents. Essential oils and aerial plant parts were the main components studied. Nevertheless, the most frequently reported constituents were xanthomicrol, limonene, luteolin, geranial, apigenin, and calycopterin. A number of medicinal properties were reported among them antioxidant, antibacterial, anticancerous, antinociceptive, antihyperlipidemic, antispasmodic, cytotoxic, and immunomodulatory effects. The plant was also reported to be a remedy for inflammatory pain, headaches, congestion, liver disorders, ulcer, fever, renal pain, dyspepsia, stomach ache, abdominal pain, joints pains, muscle spasm, congestion, bloating, and wound healing effects, among others. Conclusion. This review has shown that D. kotschyi is an important medicinal plant with a large number of active constituents and great potential to safeguard human and animal health in Iran. However, over utilization of the D. kotschyi plant is already endangering its existence. Nevertheless, more studies need be conducted across the country.

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Investigation on Chemical Composition, Antimicrobial, Antioxidant, and Cytotoxic Properties of Essential Oil From Dracocephalum Kotschyi Boiss

Cited by 24 publications

References 28 publications

Biological Activity and Chemical Composition of the Essential Oil of Nepeta cataria L.

Biological Activity and Chemical Composition of the Essential Oil of Nepeta cataria L.

Cytotoxic Activity of Essential Oils of Some Species from Lamiaceae Family

Medicinal Properties and Active Constituents of Dracocephalum kotschyi and Its Significance in Iran: A Systematic Review

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