Investigation of viruses in Diporeia spp. from the Laurentian Great Lakes and Owasco Lake as potential stressors of declining populations

Hewson, Ian; Eaglesham, James B.; Höök, Tomas O.; LaBarre, Brenna A.; Sepúlveda, Marı́a S.; Thompson, Philip D.; Watkins, James M.; Rudstam, Lars G.

doi:10.1016/j.jglr.2013.06.006

Cited by 37 publications

(30 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these, between 0.06 and 88.03% matched viral genomes, with the remaining sequences corresponding to bacterial, archaeal, or eukaryotic genome fragments. This is similar to the results of previous studies that utilized similar viral genome isolation protocols, and likely results from DNA released from host, bacterial, and/or archaeal cells during the tissue homogenization process (Hewson et al, 2011a(Hewson et al, , 2013Gudenkauf et al, 2014). The low number of viral annotations may be a result of lack of representative viral genomes in public databases (Weynberg et al, 2014).…”

Section: Metagenomic Annotationsupporting

confidence: 85%

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Gudenkauf

Hewson

2016

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

Viruses are the most abundant biological entities on Earth, killing 10-20% of oceanic biomass each day. However, despite their ecological importance, viruses inhabiting many echinoderms, cnidarians, urochordates, and marine arthropods have not been investigated with significant breadth. We conducted a broad survey of the viral assemblages inhabiting these hosts through viral metagenomics and phylogenetic analysis. Results indicate that different invertebrate groups harbor distinct viral assemblages. Interestingly, however, no significant difference is observed between the viral assemblages of echinoderms and arthropods. These similarities and differences may be due to cellular, immunological, geographical, and ecological differences amongst host phyla, although mechanistic determination is beyond the purview of this work. Additionally, we present evidence of the detection of several viral families that have not yet been observed in these hosts. Finally, we confirm the result of previous investigation that method of library construction significantly biases metagenomic results by altering the representation of ssDNA and dsDNA viral genomes.

show abstract

Section: Metagenomic Annotationsupporting

confidence: 85%

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Gudenkauf

Hewson

2016

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally, there is a productive deep chlorophyll layer in the lake, and this production has probably increased over time . Other possibilities include viral diseases or some other unknown pathogen (Hewson et al, 2013). As long as the exact mechanism remains unknown, the coupling of the decline in Diporeia and the increase in Quagga Mussel remains a correlation.…”

Section: Discussionmentioning

confidence: 99%

Long-term trends in Lake Ontario's benthic macroinvertebrate community from 1994–2008

Birkett

Lozano

Rudstam

2015

Aquatic Ecosystem Health &Amp; Management

View full text Add to dashboard Cite

The benthic macroinvertebrate community of Lake Ontario was assessed through a lakewide survey in 2008. Diporeia was very rare throughout the lake at all depths in 2008, and only four of 52 locations had densities >100 m ¡2 , all of them at depths >90 m. The maximum density of Diporeia found at any location was at 257 m ¡2 in 2008, which can be compared to maximum densities of 13,280 m ¡2 observed in 1994. Lakewide Diporeia abundance declined with an additional order of magnitude from an average of 342 m ¡2 in 2003 to 21 m ¡2 in 2008. The Quagga Mussel (D. rostriformis bugensis) dominated the benthic macroinvertebrate community in 2008, comprising over 70% of the density and 98% of the biomass. No Zebra Mussels were identified in the 2008 samples. Quagga Mussels, Oligochaetes and Chironomids were most abundant between 31 and 90 m. Sphaeriids were rare at all depths, but were more abundant at sites deeper than 90 m. Between 2003 and 2008, lakewide Dreissena abundance declined by 43% primarily due to significant declines in the 10-30 m depth region (from 6500 m ¡2 to 900 m ¡2 ). Dreissena did not decline significantly in the 30-90 m or over 90 m depth regions. The 2008 survey revealed a continued decline in Diporeia and Sphaeriid Clams, a replacement of Zebra Mussels by Quagga Mussels, and a decline in Quagga Mussels at depths shallower than 30 m. Oligochaetes and chironomids showed no significant changes since the 1990s.

show abstract

“…Only a few studies have reported the metagenomic identification of novel viruses in P. monodon, in Farfantepenaeus duorarum, in copepods and in bivalves [7,[14][15][16][17]. Apart from these economically important species, few studies were published concerning the viromes of ecological important marine invertebrates, such as ghost and volcano shrimps, tunicates, non-scleractinian corals, amphipods and echinoderms [18][19][20][21][22]. However, recent comprehensive surveys have been conducted to discover the genomic diversity of multiple DNA and RNA viruses in arthropods, including marine species [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Exploration of the virome of the European brown shrimp (Crangon crangon)

Eynde

Christiaens

Delbare

et al. 2020

Journal of General Virology

View full text Add to dashboard Cite

Crangon crangon is economically a very important species. Recently, promising culture attempts have been made, but a major problem is the uncontrollable mortality during the grow-out phase. As of yet, the life cycle of C. crangon is not closed in captivity so wild-caught individuals are used for further rearing. Therefore, it is important to investigate the virome of C. crangon both in wild-caught animals as in cultured animals. In recent years, next-generation-sequencing (NGS) technologies have been very important in the unravelling of the virome of a wide range of environments and matrices, such as soil, sea, potable water, but also of a wide range of animal species. This will be the first report of a virome study in C. crangon using NGS in combination with the NetoVIR protocol. The near complete genomes of 16 novel viruses were described, most of which were rather distantly related to unclassified viruses or viruses belonging to the Picornavirales, Bunyavirales Nudiviridae, Parvoviridae, Flaviviridae, Hepeviridae, Tombusviridae, Narnaviridae, Nodaviridae, Sobemovirus. A difference in virome composition was observed between muscle and hepatopancreatic tissue, suggesting a distinct tissue tropism of several of these viruses. Some differences in the viral composition were noted between the cultured and wild shrimp, which could indicate that in sub-optimal aquaculture conditions some viruses become more abundant. This research showed that a plethora of unknown viruses is present in C. crangon and that more research is needed to determine which virus is potentially dangerous for the culture of C. crangon.

show abstract

Investigation of viruses in Diporeia spp. from the Laurentian Great Lakes and Owasco Lake as potential stressors of declining populations

Cited by 37 publications

References 63 publications

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Long-term trends in Lake Ontario's benthic macroinvertebrate community from 1994–2008

Exploration of the virome of the European brown shrimp (Crangon crangon)

Contact Info

Product

Resources

About