Investigation of Ultrafast Configurational Photoisomerization of Bilirubin Using Femtosecond Stimulated Raman Spectroscopy

Pu, Ruihua; Wang, Ziyu; Zhu, Ruixue; Jiang, Jiaming; Weng, Tsu‐Chien; Liu, Weimin

doi:10.1021/acs.jpclett.2c03535

Cited by 7 publications

(3 citation statements)

References 58 publications

(88 reference statements)

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“…From the point of view of BR biological implications, particularly for neonatal jaundice phototherapy, from the 1970s, BR photoisomerization has been extensively studied by McDonagh and Lightner [ 117 , 118 ]. Characterization of photoisomers of BR bound to serum albumin is also important [ 119 , 120 ], and the dynamics of photoinduced configurational and structural isomerization are still worth investigating with modern spectroscopic techniques [ 121 ] since all details of such processes are not yet completely clear.…”

Section: Discussionmentioning

confidence: 99%

Insights into the Structures of Bilirubin and Biliverdin from Vibrational and Electronic Circular Dichroism: History and Perspectives

et al. 2023

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In this work we review research activities on a few of the most relevant structural aspects of bilirubin (BR) and biliverdin (BV). Special attention is paid to the exocyclic C=C bonds being in mostly Z rather than E configurations, and to the overall conformation being essentially different for BR and BV due to the presence or absence of the double C=C bond at C-10. In both cases, racemic mixtures of each compound of either M or P configuration are present in achiral solutions; however, imbalance between the two configurations may be easily achieved. In particular, results based on chiroptical spectroscopies, both electronic and vibrational circular dichroism (ECD and VCD) methods, are presented for chirally derivatized BR and BV molecules. Finally, we review deracemization experiments monitored with ECD data from our lab for BR in the presence of serum albumin and anesthetic compounds.

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Section: Discussionmentioning

confidence: 99%

Insights into the Structures of Bilirubin and Biliverdin from Vibrational and Electronic Circular Dichroism: History and Perspectives

et al. 2023

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“…141 While entangled FSRS has yet to be experimentally realized, the use of lower fluxes in these measurements is quite appealing, as is the possibility of interrogating quantum phenomena while using quantum light. FSRS has the unique ability to track structural changes in electronic excited states and has answered key questions for applications in renewable energy, 14,104 bioimaging, 6 phototherapy, 142 and beyond. New advances in ML techniques for data analysis, as well as the promise of quantum-enhanced FSRS on the horizon, demonstrate the future ability of FSRS to investigate samples with smaller cross sections, thereby opening the door even wider for molecular systems that can be investigated.…”

Section: Discussionmentioning

confidence: 99%

Mastering Femtosecond Stimulated Raman Spectroscopy: A Practical Guide

Lynch,

Das,

Alam

et al. 2023

ACS Phys. Chem Au

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Femtosecond stimulated Raman spectroscopy (FSRS) is a powerful nonlinear spectroscopic technique that probes changes in molecular and material structure with high temporal and spectral resolution. With proper spectral interpretation, this is equivalent to mapping out reactive pathways on highly anharmonic excited-state potential energy surfaces with femtosecond to picosecond time resolution. FSRS has been used to examine structural dynamics in a wide range of samples, including photoactive proteins, photovoltaic materials, plasmonic nanostructures, polymers, and a range of others, with experiments performed in multiple groups around the world. As the FSRS technique grows in popularity and is increasingly implemented in user facilities, there is a need for a widespread understanding of the methodology and best practices. In this review, we present a practical guide to FSRS, including discussions of instrumentation, as well as data acquisition and analysis. First, we describe common methods of generating the three pulses required for FSRS: the probe, Raman pump, and actinic pump, including a discussion of the parameters to consider when selecting a beam generation method. We then outline approaches for effective and efficient FSRS data acquisition. We discuss common data analysis techniques for FSRS, as well as more advanced analyses aimed at extracting small signals on a large background. We conclude with a discussion of some of the new directions for FSRS research, including spectromicroscopy. Overall, this review provides researchers with a practical handbook for FSRS as a technique with the aim of encouraging many scientists and engineers to use it in their research.

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“…Phototherapy has become a necessary treatment option to quickly and effectively reduce unconjugated bilirubin and prevent bilirubin encephalopathy (2). Phototherapy causes photoisomerization of unconjugated bilirubin in superficial skin tissues to form configurational and structural isomers, both of which are water-soluble and can be directly excreted through bile and urine without liver metabolism, thereby reducing the concentration of bilirubin in the body (3). However, with the increasing popularity of phototherapy in clinical practice, there have been an increasing number of reports on its related side effects such as fever, diarrhea, rash and bronzed skin, which have gradually attracted the attention of doctors (4).…”

Section: Introductionmentioning

confidence: 99%

Beneficial changes in gut microbiota after phototherapy for neonatal hyperbilirubinemia

Wu,

Jiang,

Yan

et al. 2024

Biomed Rep

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Phototherapy is the most commonly used treatment for neonatal hyperbilirubinemia (NH). Gut microbiota is involved in bilirubin metabolism; however, it is uncertain whether this is affected by phototherapy. The present study included 43 newborns with hyperbilirubinemia and collected fecal samples for high-throughput sequencing before and after phototherapy. Selection α diversity analysis was used to determine the differences in diversity and abundance between the two groups, whereas similarity was determined using β diversity analysis. Linear discriminant analysis effect size analysis was used to screen for markedly different bacteria. The structure of the gut microbiota in newborns with hyperbilirubinemia changed after phototherapy, with a significant decrease in abundance and diversity. The changes in the key bacterial species were characterized by an increase in the abundance of Streptococcus salivarius and a decrease in the abundance of Escherichia, Klebsiella pneumoniae, Rothia mucilaginosa and Streptococcus oralis. These changes mainly manifested as an increase in beneficial bacteria and a decrease in opportunistic bacteria, which may not be related to the side effects of phototherapy. These results can provide theoretical assistance for microbiological research on the later stages of NH.

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Investigation of Ultrafast Configurational Photoisomerization of Bilirubin Using Femtosecond Stimulated Raman Spectroscopy

Cited by 7 publications

References 58 publications

Insights into the Structures of Bilirubin and Biliverdin from Vibrational and Electronic Circular Dichroism: History and Perspectives

Insights into the Structures of Bilirubin and Biliverdin from Vibrational and Electronic Circular Dichroism: History and Perspectives

Mastering Femtosecond Stimulated Raman Spectroscopy: A Practical Guide

Beneficial changes in gut microbiota after phototherapy for neonatal hyperbilirubinemia

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